Effect of biochar-immobilized Bacillus licheniformis Y on the remediation of soils contaminated with petroleum hydrocarbons.

Microbial immobilization technology refers to free microorganisms are immobilized in a limited carrier by physical or chemical means to increase microbial activity. Although it is considered to be a cost-effective and sustainable method to remediate organic contaminated soils, little attention has been paid to the interaction mechanisms between biochar and microbes which are immobilized on biochar by various methods e.g. adsorption, embedding and covalent binding, and more research is needed to understand how soil microbes interact with exogenous immobilized microbes. In this study, biochar-immobilized Bacillus licheniformis Y was used to remediate soils contaminated with petroleum hydrocarbons. The results showed that the degradation ratio of petroleum hydrocarbons was highest in the soil treated with biochar with immobilized microbes. However, the concentration of petroleum hydrocarbons adsorbed onto the biochar increased rapidly in the first five days of incubation. Additionally, to investigate the differently changed microbes that existed in the biochar, we compared the abundance of microbes between biochar-only and biochar-only treated soil in days 5 and 25, as well as between biochar-immobilized bacterial pellets and biochar-only. After 25 days incubation, the majority of enriched microbes colonizing the biochar pellets were markedly different from those after 5 days incubation. At the end of incubation period, members of the genera Rhodococcus, Mycobacterium, Bacillus, Lysobacter and Brevundimonas were predominated in the biochar pellets. Furthermore, no significant differences in microbial community composition were observed between biochar with and without immobilized Bacillus licheniformis Y. These findings suggest that biochar-immobilized microbe method is an attractive approach for the highly efficient remediation of petroleum hydrocarbons-contaminated soil.

• Publication year

  2025

• Venue

  Journal of Environmental Management

• Publication date

  2025-09-10

• Fields of study

  Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.jenvman.2025.127221PMID 40934665
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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