Crucial Roles of Glu60 in Human Neuroglobin as a Guanine Nucleotide Dissociation Inhibitor and Neuroprotective Agent

Mammalian neuroglobin (Ngb) protects neuronal cells under conditions of oxidative stress. We previously showed that human Ngb acts as a guanine nucleotide dissociation inhibitor (GDI) for the α-subunits of heterotrimeric Gi/o proteins and inhibits reductions in cAMP concentration, leading to protection against cell death. In the present study, we created human E60Q Ngb mutant and clarified that Glu60 of human Ngb is a crucial residue for its GDI and neuroprotective activities. Moreover, we investigated structural and functional properties of several human Ngb mutants and demonstrated that the neuroprotective effect of human Ngb is due to its GDI activity and not due to its scavenging activity against reactive oxygen species.

• Publication year

  2013

• Venue

  PLoS ONE

• Publication date

  2013-12-23

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1371/journal.pone.0083698PMID 24376733PMCID 3871547
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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