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Evaluating yield of DNA extraction methods across different tissues of moon jellyfish Aurelia aurita (Linnaeus, 1758)

Sümeyra Zeynep Çalıcı,Buse Bayrak,Hatice Buse Yavuz,Nida Kelel,Zeynep Aslıhan Avşaroğlu,M. Aydemir

Published 2025 in Biological Diversity and Conservation

ABSTRACT

Purpose: This study aimed to optimize DNA extraction from the moon jellyfish Aurelia aurita, a gelatinous marine invertebrate, by comparing different methodologies and evaluating the influence of tissue type and Proteinase K treatment duration on DNA yield. Method: A total of 48 different combinations were tested using three extraction methods (salting-out, kit-based, and Trizol-based) across four tissue types (gonads, tentacles, epidermis, and gastrodermis). Each method was applied with varying Proteinase K incubation times (0, 1, 3, and 24 hours) to assess their impact on DNA yield and integrity. Findings: Gonadal tissues consistently provided the highest DNA concentrations, likely due to their relatively high cellular density and structural robustness. Among the methods, the salting-out protocol yielded superior results, particularly with 1–3 hours of Proteinase K incubation. In contrast, highly hydrated tissues such as epidermis and gastrodermis led to underestimated yields due to distorted wet weight measurements. Conclusion: Tissue selection, enzymatic treatment time, and extraction methodology critically affect DNA recovery from jellyfish. The salting-out method, when applied to gonadal tissue with moderate Proteinase K incubation, is recommended for efficient and reliable DNA isolation from Aurelia aurita.

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