Oleanolic acid-taxifolin self-assembled nanoparticles for effective treatment of S. aureus infections by inhibiting its internalization and reproduction.

The intracellular parasitic traits of Staphylococcus aureus (S. aureus) facilitate its evasion of both antibiotic therapies and the host immune response, frequently resulting in therapeutic failures. Herein, using oleanolic acid (OA) and taxifolin (TAX) as raw ingredients, we developed self-assembled nanoparticles (OA-TAX NPs) with the goal to resolve an intracellular infection caused by S. aureus. OA-TAX NPs prohibited the invasion of S. aureus into cells by inhibiting sortase A (SrtA) activity and achieved intracellular effective accumulation through endocytosis. Specifically, OA-TAX NPs killed up to 84.4 % of intracellular S. aureus and alleviated inflammation. In the mice mastitis model, OA-TAX NPs successfully cured the S. aureus infection, improved the polarization of M2-type macrophages and dramatically reduced inflammatory damage. Overall, Antibiotic-free OA-TAX NPs offered an innovative strategy for the management of intracellular S. aureus.

• Publication year

  2025

• Venue

  Colloids and Surfaces B: Biointerfaces

• Publication date

  2025-10-10

• Fields of study

  Medicine, Materials Science, Chemistry

• Identifiers
  DOI 10.1016/j.colsurfb.2025.115199PMID 41076853
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Dual role of Baimao-Longdan-Congrong-Fang in inhibiting Staphylococcus aureus virulence factors and regulating TNF-α/TNFR1/NF-κB/MMP9 axis.

  2025cited by this paper
• Macrophage Membrane-Coated Nanoscale Allicin for the Targeted Treatment of Drug-Resistant Bacterial Pneumonia

  2025cited by this paper
• Triple-targeting nanosystems with synergistic effects on iron Trojan horse, fluoroquinolone antibiotics, and photodynamic therapy specifically kill intracellular MRSA

  2025cited by this paper
• Bi-functional quercetin/copper nanoparticles integrating bactericidal and anti-quorum sensing properties for preventing the formation of biofilms.

  2024cited by this paper
• Small Molecule Hydrogels Loading Small Molecule Drugs from Chinese Medicine for the Enhanced Treatment of Traumatic Brain Injury.

  2024cited by this paper
• Oleanolic acid derivatives against drug-resistant bacteria and fungi by multi-targets to avoid drug resistance.

  2024cited by this paper
• Berberine and magnolol exert cooperative effects on ulcerative colitis in mice by self-assembling into carrier-free nanostructures

  2024cited by this paper
• Design and synthesis of unique indole-benzosulfonamide oleanolic acid derivatives as potent antibacterial agents against MRSA.

  2024cited by this paper
• Zwitterion-Modified MXene Quantum Dot as a Nanocarrier for Traditional Chinese Medicine Sanguinarine Delivery and Its Application for Photothermal-Chemotherapy Synergistic Antibacterial and Wound Healing.

  2024cited by this paper
• Oleanolic acid improved intestinal immune function by activating and potentiating bile acids receptor signaling in E. coli-challenged piglets

  2024cited by this paper
• Staphylococcus aureus membrane vesicles: an evolving story.

  2024cited by this paper
• Redox-sensitive self-assembling polymer micelles based on oleanolic modified hydroxyethyl starch: Synthesis, characterisation, and oleanolic release.

  2024cited by this paper
• Polyvinyl alcohol/carboxymethyl chitosan-based hydrogels loaded with taxifolin liposomes promote diabetic wound healing by inhibiting inflammation and regulating autophagy.

  2024cited by this paper
• Cryptotanshinone‐Doped Photothermal Synergistic MXene@PDA Nanosheets with Antibacterial and Anti‐Inflammatory Properties for Wound Healing

  2023cited by this paper
• Global mortality associated with 33 bacterial pathogens in 2019: a systematic analysis for the Global Burden of Disease Study 2019

  2022cited by this paper
• Antibiotics-free nanoparticles eradicate Helicobacter pylori biofilms and intracellular bacteria.

  2022cited by this paper
• Antimicrobial mechanisms of traditional Chinese medicine and reversal of drug resistance: a narrative review.

  2022cited by this paper
• Eriodictyol as a Potential Candidate Inhibitor of Sortase A Protects Mice From Methicillin-Resistant Staphylococcus aureus-Induced Pneumonia

  2021cited by this paper
• Orientin mediates protection against MRSA-induced pneumonia by inhibiting Sortase A

  2021cited by this paper
• Pure drug nano-assemblies: A facile carrier-free nanoplatform for efficient cancer therapy

  2021cited by this paper
• Plant Natural Flavonoids Against Multidrug Resistant Pathogens

  2021cited by this paper
• Taxifolin, an Inhibitor of Sortase A, Interferes With the Adhesion of Methicillin-Resistant Staphylococcal aureus

  2021cited by this paper
• Efficacy and mechanism of actions of natural antimicrobial drugs.

  2020cited by this paper
• Intracellular bacteria destruction via traceable enzymes-responsive release and deferoxamine-mediated ingestion of antibiotics.

  2020cited by this paper
• Application of Oleanolic Acid and Its Analogues in Combating Pathogenic Bacteria In Vitro/Vivo by a Two-Pronged Strategy of β-Lactamases and Hemolysins

  2020cited by this paper
• Natural Berberine-Based Chinese Herb Medicine Assembled Nanostructures with Modified Antibacterial Application.

  2019cited by this paper
• Bacteria-Targeting Nanoparticles with Microenvironment-Responsive Antibiotic Release To Eliminate Intracellular Staphylococcus aureus and Associated Infection.

  2018cited by this paper
• A review on nanosystems as an effective approach against infections of Staphylococcus aureus

  2018cited by this paper
• Nanocurcumin ameliorates Staphylococcus aureus‐induced mastitis in mouse by suppressing NF‐&kgr;B signaling and inflammation

  2018cited by this paper
• Preparation, characterization, and evaluation of 3,6-O-N-acetylethylenediamine modified chitosan as potential antimicrobial wound dressing material.

  2018cited by this paper
• Chalcone Attenuates Staphylococcus aureus Virulence by Targeting Sortase A and Alpha-Hemolysin

  2017cited by this paper
• Particle engineering for intracellular delivery of vancomycin to methicillin‐resistant Staphylococcus aureus (MRSA)‐infected macrophages

  2017cited by this paper
• Enhanced intracellular delivery and antibacterial efficacy of enrofloxacin-loaded docosanoic acid solid lipid nanoparticles against intracellular Salmonella

  2017cited by this paper
• Cell-Penetrating Peptides: From Basic Research to Clinics.

  2017cited by this paper
• Preparation and antitumor evaluation of self-assembling oleanolic acid-loaded Pluronic P105/d-α-tocopheryl polyethylene glycol succinate mixed micelles for non-small-cell lung cancer treatment

  2016cited by this paper
• Broad‐Spectrum Antimicrobial Star Polycarbonates Functionalized with Mannose for Targeting Bacteria Residing inside Immune Cells

  2016cited by this paper
• Staphylococcus aureus Infections: Epidemiology, Pathophysiology, Clinical Manifestations, and Management

  2015cited by this paper
• Nanocarriers for antibiotics: a promising solution to treat intracellular bacterial infections.

  2014cited by this paper
• Livestock-associated Staphylococcus aureus.

  2012cited by this paper
• Modulation of antibiotic resistance in bacterial pathogens by oleanolic acid and ursolic acid.

  2012cited by this paper
• Sortase as a Target of Anti-Infective Therapy

  2008cited by this paper
• Ursolic, oleanolic and betulinic acids: antibacterial spectra and selectivity indexes.

  2008cited by this paper
• Antibacterial Activity of Triterpene Acids and Semi-Synthetic Derivatives against Oral Pathogens

  2007cited by this paper
• Cellular pharmacodynamics and pharmacokinetics of antibiotics: current views and perspectives.

  2006cited by this paper
• The role of nasal carriage in Staphylococcus aureus infections.

  2005cited by this paper
• Efficacies of liposome-encapsulated streptomycin and ciprofloxacin against Mycobacterium avium-M. intracellulare complex infections in human peripheral blood monocyte/macrophages

  1992cited by this paper

• No citing papers are available for this paper.