G-quadruplex enhances the peroxidase-like activity of 2D Cu-hemin for sensitive and accurate colorimetric detection of methyl parathion.

Precise and reliable monitoring of organophosphate pesticide residues plays a critical role in safeguarding public health and environmental equilibrium. We investigated different types of DNAs with Cu-hemin and developed a G-quadruplex (G4)-regulated MOF nanozyme method. The catalytic performance of Cu-hemin@G4 nanostructures demonstrated a 11-fold enhancement in peroxidase (POD) activity over isolated Cu-hemin MOF, achieving efficient conversion of 3,3',5,5'-tetramethylbenzidine (TMB) chromogenic substrate into blue-colored oxidized derivatives. Acetylcholinesterase (AChE) and acetylcholine (ATCh) inhibited this activity, but organophosphate pesticides (OPs) restored it through enzymatic interaction. On the basis of G-quadruplex-regulated mimic enzyme activity, the proposed colorimetric method realized quantitative methyl parathion determination ranging from 0.005 μg L-1 to 0.4 μg L-1 with an ultra-low detection limit of 0.975 ng L-1. The research develops rational strategies for constructing high-performance nanozymes, advances nanozyme-based biosensors for OPs detection, and offers efficient solutions for pesticide detection in agro-ecological applications.

• Publication year

  2025

• Venue

  Food Chemistry

• Publication date

  2025-10-01

• Fields of study

  Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1016/j.foodchem.2025.146732PMID 41332021
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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