Unraveling the molecular interaction mechanisms of antifreeze peptides with ice crystals and myofibrillar proteins: Insights from multispectral and molecular simulation analyses.

This study combined multispectral techniques and computational simulations to investigate the interaction mechanisms between antifreeze peptides (AFPs), ice crystals, and myofibrillar proteins (MPs). The results showed that AFP-GDQ (THA = 2.05 °C; residual peroxidase activity 84 %) and AFP-GFA (THA = 1.98 °C; residual peroxidase activity of 70 %) were identified through screening as potentially highly active AFPs. Both peptides interacted with MP, altered the secondary structure of MP, and induced fluorescence quenching. The quenching mechanism for AFP-GDQ was static, whereas AFP-GFA exhibited dynamic quenching. In addition, molecular simulations indicated that AFP-GDQ (Asp2, Gln3, Arg5, Ala8) and AFP-GFA (Phe2, Ala3, Pro5, Ser8) could bind to both ice-crystal surfaces and MP. New insights were provided to reveal the interaction mechanism of AFP as an antifreeze agent occurring after incorporation into surimi.

• Publication year

  2025

• Venue

  International Journal of Biological Macromolecules

• Publication date

  2025-11-01

• Fields of study

  Medicine, Materials Science, Chemistry

• Identifiers
  DOI 10.1016/j.ijbiomac.2025.148881PMID 41207595
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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