Alleviating stocking density stress in juvenile rainbow trout (Oncorhynchus mykiss) through dietary nano-selenium: Effects on growth, antioxidant activity, and immune response.

Trout is a prized fish mostly cultured under high stocking densities (SD), which relates to many physiological challenges. The present study determines the significance of nano selenium (Se-N), while rearing Rainbow trout (Oncorhynchus mykiss) under various SD. Healthy O. mykiss juvenile were randomly distributed in concrete raceways in a 4 × 5 factorial experimental design. Five experimental feeds (NS0, NS1, NS2, NS3, NS4) containing 0, 1, 2, 3, and 4 mg Se-N per Kg were tested in four SDs (SD-L=168, SD-M=300, SD-H=433, SD-V=567 g/m3). Inference revealed significant effects of both SD and Se-N on growth (NWG), antioxidant activity and molecular regulation of myogenesis, and immunity. Our results show no significant effect of SD on growth at SD-L and SD-M, however at higher SD (SD-H and SD-V) Se-N role became prominent as significantly high NWG was observed in NS3 followed by NS2, NS4, NS1 and NS0, respectively. Lower cortisol in NS3 at higher SD might be the result of higher antioxidant activity (SOD, CAT, GPx), which seems to counter the chronic stress caused by SD. High SD also induced expression of immunity related genes (Lysozyme and IL-1β) especially in absence/lower inclusion of Se-N, while upregulated hsp70 expression. Finally, the dose dependent (Se-N) high expression of MyoD and gh, and a very slight effect on igf-1 justify the higher growth in NS3 by hyperplasia and not by hypertrophy. Our study suggests supplementation of 3 mg/kg Se-N in the diet of O. mykiss, especially in culture condition of high SD.

• Publication year

  2025

• Venue

  Journal of Trace Elements in Medicine and Biology

• Publication date

  2025-11-01

• Fields of study

  Agricultural and Food Sciences, Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.jtemb.2025.127798PMID 41223821
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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