The mechanism of exogenous MeJA-induced CmMYB36-CmVND1 module regulation in the graft union healing process of melon grafted onto squash.

Exogenous methyl jasmonate (MeJA) plays a significant role in plant growth, development, and defense responses and is crucial for tissue reconnection in grafted plants. However, the mechanism of MeJA participation in the graft union healing of melon grafted onto squash remained unclear. Therefore, elucidating the regulatory mechanism of exogenous MeJA is crucial for enhancing graft union healing efficiency and improving the quality of grafted plants. Our results indicated that exogenous MeJA application to melon scions alone, as well as simultaneous application to both melon scions and squash rootstocks, accelerates the graft union healing process between melon and squash. CmVND1 can respond to MeJA through the CGTCA motif element, and exogenous MeJA induction can further increase the expression of CmVND1. Our transient silencing assays further indicated that CmVND1 can regulate the graft union healing process and improve the healing efficiency. Finally, through yeast one-hybrid and GUS staining assays, we found that CmMYB36 binds to the promoter of its downstream gene CmVND1 and regulates its transcriptional expression levels, thereby accelerating the graft union healing process. In summary, our findings indicated that CmMYB36 participated in the graft union healing process of melon grafted onto squash by upregulating the target gene CmVND1, thereby improving the success rate of grafting and offering practical targets to improve grafting efficiency in melon cultivation.

• Publication year

  2025

• Venue

  Plant physiology and biochemistry : PPB

• Publication date

  2025-11-05

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.plaphy.2025.110641PMID 41207214
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Transcriptomic analysis provides an insight into the function of CmGH9B3, a key gene of β‐1, 4‐glucanase, during the graft union healing of oriental melon scion grafted onto squash rootstock

  2024cited by this paper
• Aquaporin CmPIP2; 3 links H2O2 signal and antioxidation to modulate trehalose-induced cold tolerance in melon seedlings.

  2024cited by this paper
• The MdCBF1/2-MdTST1/2 module regulates sugar accumulation in response to low temperature in apple.

  2024cited by this paper
• Exogenous Melatonin Application Accelerated the Healing Process of Oriental Melon Grafted onto Squash by Promoting Lignin Accumulation

  2024cited by this paper
• Plant Grafting: Molecular Mechanisms and Applications.

  2023cited by this paper
• A genome‐wide analysis of the GRAS gene family in upland cotton and a functional study of the role of the GhGRAS55 gene in regulating early maturity in cotton

  2023cited by this paper
• Simple differential staining method of paraffin-embedded plant sections with safranin-alcian blue

  2023cited by this paper
• Transcriptomic analysis and physiological characteristics of exogenous naphthylacetic acid application to regulate the healing process of oriental melon grafted onto squash

  2022cited by this paper
• Long-distance control of the scion by the rootstock under drought stress as revealed by transcriptome sequencing and mobile mRNA identification

  2022cited by this paper
• OUP accepted manuscript

  2022cited by this paper
• Three OsMYB36 members redundantly regulate Casparian strip formation at the root endodermis.

  2022cited by this paper
• Light-responsive transcription factor PpWRKY44 induces anthocyanin accumulation by regulating PpMYB10 expression in pear

  2022cited by this paper
• Gene regulatory networks for compatible versus incompatible grafts identify a role for SlWOX4 during junction formation

  2021cited by this paper
• Cytological Studies on Graft Union Development with Perennial Chilli Rootstocks

  2021cited by this paper
• iTRAQ-based quantitative proteomics analysis of cold stress-induced mechanisms in grafted watermelon seedlings.

  2019cited by this paper
• Transcription Factor CsWIN1 Regulates Pericarp Wax Biosynthesis in Cucumber Grafted on Pumpkin

  2019cited by this paper
• Plant and Cell Physiology's 2018 Database Issue and Beyond.

  2018cited by this paper
• Plant and Cell Physiology's 2018 Database Issue and Beyond.

  2018cited by this paper
• Improving vanadium stress tolerance of watermelon by grafting onto bottle gourd and pumpkin rootstock

  2018cited by this paper
• Transcription Factors VND1-VND3 Contribute to Cotyledon Xylem Vessel Formation1[OPEN]

  2017cited by this paper
• NO accumulation alleviates H2 O2 -dependent oxidative damage induced by Ca(NO3 )2 stress in the leaves of pumpkin-grafted cucumber seedlings.

  2017cited by this paper
• Two-Step Functional Innovation of the Stem-Cell Factors WUS/WOX5 during Plant Evolution

  2016cited by this paper
• Grafting cucumber onto luffa improves drought tolerance by increasing ABA biosynthesis and sensitivity

  2016cited by this paper
• Genome-wide identification and comparative analysis of grafting-responsive mRNA in watermelon grafted onto bottle gourd and squash rootstocks by high-throughput sequencing

  2015cited by this paper
• A Developmental Framework for Graft Formation and Vascular Reconnection in Arabidopsis thaliana.

  2015cited by this paper
• Multiple classes of transcription factors regulate the expression of VASCULAR-RELATED NAC-DOMAIN7, a master switch of xylem vessel differentiation.

  2015cited by this paper
• The MYB36 transcription factor orchestrates Casparian strip formation

  2015cited by this paper
• Arabidopsis NAC Domain Proteins, VND1 to VND5, Are Transcriptional Regulators of Secondary Wall Biosynthesis in Vessels

  2014cited by this paper
• Differential activation of sporamin expression in response to abiotic mechanical wounding and biotic herbivore attack in the sweet potato

  2014cited by this paper
• Contributions of roots and rootstocks to sustainable, intensified crop production.

  2013cited by this paper
• Graft-union development: a delicate process that involves cell–cell communication between scion and stock for local auxin accumulation

  2012cited by this paper
• The AP2/ERF transcription factor WIND1 controls cell dedifferentiation in Arabidopsis.

  2011cited by this paper
• Spatially selective hormonal control of RAP2.6L and ANAC071 transcription factors involved in tissue reunion in Arabidopsis

  2011cited by this paper
• Current status of vegetable grafting: Diffusion, grafting techniques, automation

  2010cited by this paper
• Global analysis of direct targets of secondary wall NAC master switches in Arabidopsis.

  2010cited by this paper
• TDIF Peptide Signaling Regulates Vascular Stem Cell Proliferation via the WOX4 Homeobox Gene in Arabidopsis[W]

  2010cited by this paper
• Jasmonate perception by inositol phosphate-potentiated COI1-JAZ co-receptor

  2010cited by this paper
• Signals involved in tuber wound-healing

  2009cited by this paper
• Plant CLE peptides from two distinct functional classes synergistically induce division of vascular cells

  2008cited by this paper
• Tracheary element differentiation.

  2007cited by this paper
• Jasmonates: an update on biosynthesis, signal transduction and action in plant stress response, growth and development.

  2007cited by this paper
• Transcription switches for protoxylem and metaxylem vessel formation.

  2005cited by this paper
• Involvement of components of the phospholipid-signaling pathway in wound-induced phenylpropanoid metabolism in lettuce (Lactuca sativa) leaf tissue

  2005cited by this paper
• Evolution of Water Transport and Xylem Structure

  2003cited by this paper

• No citing papers are available for this paper.