Fine Mapping of the All‐Stage Stripe Rust Resistance Gene Yr4EL and Its Utilization in Wheat Resistance Breeding

Tetraploid Thinopyrum elongatum represents a valuable tertiary genetic pool for wheat improvement, harbouring numerous valuable agronomic traits. Our previous study identified Yr4EL, which confers all‐stage resistance to stripe rust, was initially characterized from the tetraploid Th. elongatum chromosome (chr) 4E. To further fine‐map Yr4EL, we generated 140 chr 4E structural variants using 60Co‐γ irradiation, ph1b‐induced, and double monosomy centromere breakage‐fusion methods. All variants were cytogenetically characterized using genomic in situ hybridization and fluorescence in situ hybridization. These variants comprised 90 distinct chr 4E structural variants, including 20 fragment deletions, 14 large segment translocations, 19 whole‐arm translocations, 10 chr 4E insertion translocations, and 27 terminal small fragment translocations. A high‐resolution chr 4E physical map was constructed using molecular markers, delineated into 15 distinct bins. Subsequently, the diploid Th. elongatum reference genome (ASM1179987v1) enabled the fine‐mapping of Yr4EL to a 1.8 Mb interval within the distal chr 4EL. Transcriptomic profiling, evolutionary analysis, and qRT‐PCR validation identified five genes that might be the Yr4EL candidate. In addition, we deployed Yr4EL into susceptible common wheat varieties, conferring effective stripe rust resistance without a yield penalty, demonstrating its high potential value for wheat breeding programmes.

• Publication year

  2025

• Venue

  Plant, Cell and Environment

• Publication date

  2025-11-09

• Fields of study

  Agricultural and Food Sciences, Medicine, Biology

• Identifiers
  DOI 10.1111/pce.70278PMID 41208146
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The Spectrum of Diverse Disease-Resistance Genes Cloned and Characterized in the Triticeae Tribe.

  2025influential reference
• Host-Parasite Interaction in Flax Rust-Its Genetics and Other Implications.

  2025cited by this paper
• A head-to-head NLR gene pair from wild emmer confers stripe rust resistance in wheat

  2025cited by this paper
• AcRLK2P-1, an LRR receptor protein kinase gene from Agropyron cristatum, confers leaf rust resistance in wheat

  2024cited by this paper
• Coordinated function of paired NLRs confers Yr84-mediated stripe rust resistance in wheat

  2024cited by this paper
• Pm57 from Aegilops searsii encodes a tandem kinase protein and confers wheat powdery mildew resistance

  2024cited by this paper
• Development, identification, and utilization of wheat–tetraploid Thinopyrum elongatum 4EL translocation lines resistant to stripe rust

  2024influential reference
• The wheat stripe rust resistance gene YrNAM is Yr10

  2024cited by this paper
• A single NLR gene confers resistance to leaf and stripe rust in wheat

  2024cited by this paper
• Development of wheat-tetraploid Thinopyrum elongatum 4EL small fragment translocation lines with stripe rust resistance gene Yr4EL

  2024cited by this paper
• A pathogen-induced putative NAC transcription factor mediates leaf rust resistance in barley

  2023cited by this paper
• Origin and evolution of the bread wheat D genome

  2023cited by this paper
• Development of novel wheat-rye 6RS small fragment translocation lines with powdery mildew resistance and physical mapping of the resistance gene PmW6RS

  2023cited by this paper
• Dissection of a rapidly evolving wheat resistance gene cluster by long-read genome sequencing accelerated the cloning of Pm69

  2023cited by this paper
• Development and Characterization of a Novel Wheat–Tetraploid Thinopyrum elongatum 6E (6D) Disomic Substitution Line with Stripe Rust Resistance at the Adult Stage

  2023cited by this paper
• Fine mapping and transcriptome sequencing reveal candidate genes conferring all-stage resistance to stripe rust on chromosome arm 1AL in Chinese wheat landrace AS1676

  2023cited by this paper
• Systemic development of wheat-Thinopyrum elongatum translocation lines and their deployment in wheat breeding for Fusarium head blight resistance.

  2023cited by this paper
• Introgression of chromosome 6PL terminal segment from Agropyron cristatum to increase both grain number and grain weight in wheat

  2023cited by this paper
• Unconventional R proteins in the botanical tribe Triticeae.

  2022cited by this paper
• Long-read genome sequencing of bread wheat facilitates disease resistance gene cloning

  2022cited by this paper
• Discovery of stripe rust resistance with incomplete dominance in wild emmer wheat using bulked segregant analysis sequencing

  2022cited by this paper
• Distribution, Polymorphism and Function Characteristics of the GST-Encoding Fhb7 in Triticeae

  2022cited by this paper
• Establishment and identification of six wheat-Thinopyrum ponticum disomic addition lines derived from partial amphiploid Xiaoyan 7430

  2022cited by this paper
• Mapping of the novel powdery mildew resistance gene Pm2Mb from Aegilops biuncialis based on ph1b-induced homoeologous recombination

  2022cited by this paper
• Chromosomal mapping of a locus associated with adult-stage resistance to powdery mildew from Agropyron cristatum chromosome 6PL in wheat

  2022cited by this paper
• Development and Identification of a Novel Wheat-Thinopyrum scirpeum 4E (4D) Chromosomal Substitution Line with Stripe Rust and Powdery Mildew Resistance.

  2021cited by this paper
• A membrane-bound ankyrin repeat protein confers race-specific leaf rust disease resistance in wheat

  2021cited by this paper
• NLR immune receptors and diverse types of non-NLR proteins control race-specific resistance in Triticeae.

  2021cited by this paper
• Cytogenetic identification and molecular marker development for the novel stripe rust-resistant wheat–Thinopyrum intermedium translocation line WTT11

  2021cited by this paper
• Horizontal gene transfer of Fhb7 from fungus underlies Fusarium head blight resistance in wheat

  2020cited by this paper
• The Resurgence of Introgression Breeding, as Exemplified in Wheat Improvement

  2020cited by this paper
• Characterization of a wheat–tetraploid Thinopyrum elongatum 1E(1D) substitution line K17–841-1 by cytological and phenotypic analysis and developed molecular markers

  2019influential reference
• Physical Mapping of Stem Rust Resistance Gene Sr52 from Dasypyrum villosum Based on ph1b-Induced Homoeologous Recombination

  2019cited by this paper
• An ancestral NB-LRR with duplicated 3′UTRs confers stripe rust resistance in wheat and barley

  2019cited by this paper
• Cloning of the wheat Yr15 resistance gene sheds light on the plant tandem kinase-pseudokinase family

  2018cited by this paper
• Production and Identification of Wheat-Agropyron cristatum 2P Translocation Lines

  2016influential reference
• Molecular Mapping of the Stripe Rust Resistance Gene Yr69 on Wheat Chromosome 2AS.

  2016cited by this paper
• Cytological and molecular analysis of wheat - Agropyron cristatum translocation lines with 6P chromosome fragments conferring superior agronomic traits in common wheat.

  2016cited by this paper
• Characterization and genome changes of new amphiploids from wheat wide hybridization.

  2015cited by this paper
• Physical mapping of chromosome 4J of Thinopyrum bessarabicum using gamma radiation-induced aberrations

  2015influential reference
• Chromosomal Location and Comparative Genomics Analysis of Powdery Mildew Resistance Gene Pm51 in a Putative Wheat-Thinopyrum ponticum Introgression Line

  2014cited by this paper
• Introduction of chromosome segment carrying the seed storage protein genes from chromosome 1V of Dasypyrum villosum showed positive effect on bread-making quality of common wheat

  2014cited by this paper
• Molecular cytogenetic characterization and stem rust resistance of five wheat-Thinopyrum ponticum partial amphiploids.

  2014cited by this paper
• Radiation-induced translocations with reduced Haynaldia villosa chromatin at the Pm21 locus for powdery mildew resistance in wheat

  2013cited by this paper
• Induction of 4VS chromosome recombinants using the CS ph1b mutant and mapping of the wheat yellow mosaic virus resistance gene from Haynaldia villosa

  2013influential reference
• Recent advances in alien gene transfer in wheat

  2004cited by this paper
• Genetic diversity of winter wheat in Shaanxi province, China, and other common wheat germplasm pools

  2002cited by this paper
• Chromosomal location of yellow rust resistance gene(s) inTriticum aestivum-Lophopyrum elongatum substitution lines

  1999cited by this paper
• The barley Mlo gene: a novel control element of plant pathogen resistance.

  1997cited by this paper
• Virulence, aggressiveness, evolution and distribution of races of Puccinia striiformis (the cause of stripe rust of wheat) in North America, 1968-87

  1992cited by this paper
• Phylogenetic relationships between chromosomes of wheat and chromosome 2E of Elytrigia elongata

  1984cited by this paper
• Induction

  1968cited by this paper
• The transfer of leaf-rust resistance from Aegilops umbellulata to wheat.

  1956cited by this paper
• Host-parasite interaction in flax rust–its genetics and other implications

  1955cited by this paper

• Development and Characterization of Novel St-R Translocation Triticale from a Trigeneric Hybrid

  2026cites this paper
• A multi-layered systems biology framework reveals dual-phased regulators and hormonal crosstalk underlying soybean cold tolerance

  2025cites this paper