SCIENCE@home

Omnidirectional 3D Printing of Anisotropic Nanofibrous Peptide Hydrogels

Adam C. Farsheed,Jonathan T. Makhoul,Danielle Chew-Martinez,Eros Maldonado,Justin Liu,L. T. Yu,E. Gorostieta-Salas,Jeffrey R. Jones,F. H. Gage,J. Hartgerink

Published 2025 in bioRxiv

ABSTRACT

Anisotropic biological tissues contain hierarchical complexity from the nano to macro length scales. While novel fabrication strategies have advanced the creation of biomimetic architectures, most rely on biologically derived polymers that possess inherent batch-to-batch variability. Here, we fabricate omnidirectional anisotropic nanofibrous hydrogels using synthetic, self-assembling MultiDomain Peptides (MDPs). Using support bath-assisted extrusion 3D printing, MDP hydrogels are created with control over nanometer-scale fibrous alignment, ~150 µm-scale print resolution, and centimeter-scale 3D architecture. Further, scaffold anisotropy is tuned by adjusting the ionic strength of the support bath, allowing fiber alignment to be decoupled from extrusion shear force and the ink used. Applying these hydrogels to in vitro tissue engineering, fabricated anisotropic hydrogels are shown to guide the alignment of multiple cell types within complex 3D prints. Furthermore, the gels are demonstrated to support the growth of human embryonic stem cell-derived cardiomyocytes into functional tissue. Collectively, this work introduces a platform for engineering anisotropic peptide hydrogels with hierarchical complexity, offering broad potential for bottom-up fabrication of functional human tissues in vitro.

PUBLICATION RECORD

CITATION MAP

EXTRACTION MAP

CLAIMS

  • No claims are published for this paper.

CONCEPTS

  • No concepts are published for this paper.

REFERENCES

Showing 1-68 of 68 references · Page 1 of 1

CITED BY

  • No citing papers are available for this paper.

Showing 0-0 of 0 citing papers · Page 1 of 1