Genome synthesis, assembly, and rebooting of therapeutically useful high G+C% mycobacteriophages

Significance Synthetic construction of mycobacteriophages will enhance their utility as diagnostic and therapeutic agents. De novo synthesis of high G+C% DNA fragments that can be readily assembled using Golden Gate Assembly and then rebooted in Mycobacterium smegmatis provide facile methods for construction of synthetic phages, as well as opportunities for readily constructing mutant and recombinant phages with enhanced therapeutic utility.

• Publication year

  2025

• Venue

  Proceedings of the National Academy of Sciences of the United States of America

• Publication date

  2025-11-10

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1073/pnas.2523871122PMID 41213020PMCID 12646305
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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