miRNA-targeted auxin nuclear signalling elements orchestrate flower fate and drought response in yellow lupine

Yellow lupine (Lupinus luteus L.) is a promising protein crop, but domestication has increased flower abscission. Flowers in higher whorls are more likely to abscise, providing a model to study factors influencing flower fate before abscission zone formation. We hypothesise that auxin localisation and miRNA-modulated signal transduction orchestrate flower development and abscission, particularly under drought. We investigated the indole-3-acetic acid (IAA) distribution in ovules and tested the effects of IAA, NPA (auxin transport inhibitor), and PCIB (auxin signalling blocker) on flower retention. Bioinformatic analyses of transcriptome data profiled miRNA-regulated auxin pathway elements (TIR1/AFBs, ARFs), with domain structures analysed for functional divergence. Selected miRNA-mRNA modules were analysed for their role in inflorescence drought response. IAA accumulation exhibited slight differences in upper pre-anthesis flowers. In the field experiment, PCIB treatment led to increased retention of generative organs, suggesting the vital role of auxin nuclear signalling in flower fate. Bioinformatic analyses identified miRNAs (MIR393, MIR160, MIR167, one novel) regulating TIR1/AFB and ARF6,8,17,18. qPCR revealed the differential expression of AFB3-, ARF6-, and ARF18-encoding transcripts and corresponding miR393, miR167, and miR160 during flower development and drought stress. However, miRNA-target expression lacked a strictly negative correlation, indicating other regulating players.

• Publication year

  2025

• Venue

  Scientific Reports

• Publication date

  2025-11-10

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1038/s41598-025-22989-xPMID 41214051PMCID 12603313
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The Role of FveAFB5 in Auxin-Mediated Responses and Growth in Strawberries

  2024cited by this paper
• NnARF17 and NnARF18 from lotus promote root formation and modulate stress tolerance in transgenic Arabidopsis thaliana

  2024cited by this paper
• Exploring the response of yellow lupine (Lupinus luteus L.) root to drought mediated by pathways related to phytohormones, lipid, and redox homeostasis

  2024cited by this paper
• Unveiling the molecular symphony: microRNA160a-Auxin Response Factor 18 module orchestrates low potassium tolerance in banana (Musa acuminata L.).

  2024cited by this paper
• The drought-responsive factor MdDREB2A affects root development by directly regulating the transcription of MdARF6

  2023cited by this paper
• Insights into microRNA regulation of flower coloration in a lily cultivar Vivian petal

  2023cited by this paper
• RhAGL24 Regulating Auxin-Related Gene RhARF18 Affects Stamen Petaloidy in Rose

  2022cited by this paper
• R-loops at microRNA encoding loci promote co-transcriptional processing of pri-miRNAs in plants

  2022cited by this paper
• Adenylate cyclase activity of TIR1/AFB auxin receptors in plants

  2022cited by this paper
• Interactive Tree Of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation

  2021cited by this paper
• Genome-wide identification of auxin response factor (ARF) gene family and the miR160-ARF18-mediated response to salt stress in peanut (Arachis hypogaea L.).

  2021cited by this paper
• Current status of microRNA-mediated regulation of drought stress responses in cereals.

  2021cited by this paper
• MEGA11: Molecular Evolutionary Genetics Analysis Version 11

  2021cited by this paper
• AUXIN RESPONSE FACTOR 18–HISTONE DEACETYLASE 6 module regulates floral organ identity in rose (Rosa hybrida)

  2021cited by this paper
• RCSB Protein Data Bank: powerful new tools for exploring 3D structures of biological macromolecules for basic and applied research and education in fundamental biology, biomedicine, biotechnology, bioengineering and energy sciences

  2020cited by this paper
• Auxin and Flower Development: A Blossoming Field.

  2020cited by this paper
• Identification of key sequence features required for microRNA biogenesis in plants

  2020cited by this paper
• Drought Disrupts Auxin Localization in Abscission Zone and Modifies Cell Wall Structure Leading to Flower Separation in Yellow Lupine

  2020cited by this paper
• Disruption of the Auxin Gradient in the Abscission Zone Area Evokes Asymmetrical Changes Leading to Flower Separation in Yellow Lupine

  2020cited by this paper
• LuluDB—The Database Created Based on Small RNA, Transcriptome, and Degradome Sequencing Shows the Wide Landscape of Non-coding and Coding RNA in Yellow Lupine (Lupinus luteus L.) Flowers and Pods

  2020influential reference
• Integrated Analysis of Small RNA, Transcriptome and Degradome Sequencing Provides New Insights into Floral Development and Abscission in Yellow Lupine (Lupinus luteus L.)

  2019influential reference
• Integration of sRNA, degradome, transcriptome analysis and functional investigation reveals gma-miR398c negatively regulates drought tolerance via GmCSDs and GmCCS in transgenic Arabidopsis and soybean

  2019cited by this paper
• Molecular and Hormonal Aspects of Drought-Triggered Flower Shedding in Yellow Lupine

  2019cited by this paper
• NGL viewer: web‐based molecular graphics for large complexes

  2018cited by this paper
• Naphthylphthalamic acid and the mechanism of polar auxin transport

  2018influential reference
• Essential Roles of Local Auxin Biosynthesis in Plant Development and in Adaptation to Environmental Changes.

  2018cited by this paper
• De novo Transcriptome Profiling of Flowers, Flower Pedicels and Pods of Lupinus luteus (Yellow Lupine) Reveals Complex Expression Changes during Organ Abscission

  2017cited by this paper
• Environmental impact of omnivorous, ovo-lacto-vegetarian, and vegan diet

  2017cited by this paper
• Origin and evolution of the nuclear auxin response system

  2017cited by this paper
• SlPIN1 regulates auxin efflux to affect flower abscission process

  2017cited by this paper
• Tomographic docking suggests the mechanism of auxin receptor TIR1 selectivity

  2016cited by this paper
• Structural Biology of Nuclear Auxin Action.

  2016cited by this paper
• Overexpression of plum auxin receptor PslTIR1 in tomato alters plant growth, fruit development and fruit shelf-life characteristics

  2016cited by this paper
• The PB1 Domain in Auxin Response Factor and Aux/IAA Proteins: A Versatile Protein Interaction Module in the Auxin Response[OPEN]

  2015influential reference
• Cleavage of INDOLE-3-ACETIC ACID INDUCIBLE28 mRNA by MicroRNA847 Upregulates Auxin Signaling to Modulate Cell Proliferation and Lateral Organ Growth in Arabidopsis

  2015cited by this paper
• Structural basis for DNA binding specificity by the auxin-dependent ARF transcription factors.

  2014cited by this paper
• Down-regulation of AUXIN RESPONSE FACTORS 6 and 8 by microRNA 167 leads to floral development defects and female sterility in tomato

  2014cited by this paper
• Mechanisms regulating auxin action during fruit development.

  2014cited by this paper
• The I-TASSER Suite: protein structure and function prediction

  2014cited by this paper
• Molecular basis for AUXIN RESPONSE FACTOR protein interaction and the control of auxin response repression

  2014cited by this paper
• nicolaus copernicus university in torun ( Poland )

  2013cited by this paper
• A comparative assessment and analysis of 20 representative sequence alignment methods for protein structure prediction

  2013cited by this paper
• The Manipulation of Auxin in the Abscission Zone Cells of Arabidopsis Flowers Reveals That Indoleacetic Acid Signaling Is a Prerequisite for Organ Shedding1[C][W][OA]

  2013cited by this paper
• Polar auxin transport: models and mechanisms

  2013cited by this paper
• A combinatorial TIR1/AFB-Aux/IAA co-receptor system for differential sensing of auxin

  2012cited by this paper
• A plant microRNA regulates the adaptation of roots to drought stress

  2012cited by this paper
• miR393 and Secondary siRNAs Regulate Expression of the TIR1/AFB2 Auxin Receptor Clade and Auxin-Related Development of Arabidopsis Leaves1[W][OA]

  2011cited by this paper
• A cellular expression map of the Arabidopsis AUXIN RESPONSE FACTOR gene family.

  2011cited by this paper
• Cellular responses to auxin: division versus expansion.

  2010cited by this paper
• The role of floral organs in carpels, an Arabidopsis loss-of-function mutation in MicroRNA160a, in organogenesis and the mechanism regulating its expression.

  2010cited by this paper
• Arabidopsis auxin response factor6 and 8 regulate jasmonic acid biosynthesis and floral organ development via repression of class 1 KNOX genes.

  2010cited by this paper
• Auxin perception--structural insights.

  2010cited by this paper
• Microarray Analysis of the Abscission-Related Transcriptome in the Tomato Flower Abscission Zone in Response to Auxin Depletion1[C][W][OA]

  2010cited by this paper
• Complex regulation of the TIR1/AFB family of auxin receptors

  2009cited by this paper
• TOPLESS Mediates Auxin-Dependent Transcriptional Repression During Arabidopsis Embryogenesis

  2008cited by this paper
• Mechanism of auxin perception by the TIR1 ubiquitin ligase

  2007cited by this paper
• Repression of AUXIN RESPONSE FACTOR10 by microRNA160 is critical for seed germination and post-germination stages.

  2007cited by this paper
• A Plant miRNA Contributes to Antibacterial Resistance by Repressing Auxin Signaling

  2006cited by this paper
• Arabidopsis microRNA167 controls patterns of ARF6 and ARF8 expression, and regulates both female and male reproduction

  2006cited by this paper
• Plant development is regulated by a family of auxin receptor F box proteins.

  2005cited by this paper
• Plant Development Is Regulated by a Family of Auxin Receptor F Box Proteins

  2005cited by this paper
• Role of auxin in regulating Arabidopsis flower development

  2005cited by this paper
• AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

  2005cited by this paper
• Auxin response factors ARF6 and ARF8 promote jasmonic acid production and flower maturation

  2005cited by this paper
• WebLogo: a sequence logo generator.

  2004cited by this paper
• The Roles of Auxin Response Factor Domains in Auxin-Responsive Transcription Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.008417.

  2003cited by this paper
• p-Chlorophenoxyisobutyric Acid Impairs Auxin Response in Arabidopsis Root1

  2003cited by this paper
• Dimerization and DNA binding of auxin response factors.

  1999cited by this paper
• Activation and repression of transcription by auxin-response factors.

  1999cited by this paper
• Abscission-Accelerators in Lupins (Lupinus Luteus L.)

  1959cited by this paper
• Abscission-Accelerators in Lupins (Lupinus Luteus L.)

  1959cited by this paper

• No citing papers are available for this paper.