Enhanced Control of Bacterial Wilt Disease in Tomato Plant via Transport and Penetration Tracking by Aid of 99mTc-Labeled Ralstonia solanacearum-Specific Bacteriophage.

In order to reduce the development of antibiotic and chemical resistance in bacterial phytopathogens like Ralstonia solanacearum, which causes bacterial wilt disease, bacteriophages are a safe and efficient biocontrol method. This research will explore how to use radioisotopes to track the bacteriophage absorption by plants from roots to leaves as a means for improving phage persistence and thereby the control of bacterial wilt disease caused by R. solanacearum. We have investigated the uptake and delivery of R. solanacearum-specific bacteriophages in tomato plants. Transferring phage through roots to leaves showed a gradual increase over time until reaching the maximal at 120 min, where the percentage of bacteriophage reaches 21.95% of the total amount of the injected radiation dose, which is considered a good sign for the ability of bacteriophage to reach the leaves and infect the pathogen through its transport system if injected directly into soil. Demonstrating in-plant translocation of R. solanacearum-specific bacteriophages and their effect of reducing bacterial wilt symptoms may significantly contribute to a better control of R. solanacearum and promote further investigations on the penetration and translocation of phages into plants.

• Publication year

  2025

• Venue

  Journal of labelled compounds & radiopharmaceuticals

• Publication date

  2025-11-01

• Fields of study

  Biology, Agricultural and Food Sciences, Medicine, Environmental Science

• Identifiers
  DOI 10.1002/jlcr.4169PMID 41217142
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Influence of some chemicals and solvents on the lytic activity and the adsorption of bacteriophages on Pectobacterium carotovoroum Subsp. carotovorum

  2024cited by this paper
• Synthesis and Characterization of Lemon Leaf Extract-Mediated Silver Nanoparticles: An Environmentally Friendly Approach with Enhanced Antibacterial Efficacy

  2024cited by this paper
• Bacteriophages as Promising Agents for Biocontrol of Ralstonia solanacearum Causing Bacterial Wilt Disease

  2023cited by this paper
• Anti-cancer, anti-inflammatory and antioxidant effects of Vit-A/C@SeNPs in mutual diethylnitrosamine and carbon tetrachloride induced hepatocellular damage in albino rats

  2022cited by this paper
• Preparation and preliminary evaluation study of [131I]iodocolchicine-gallic-AuNPs: a potential scintigraphic agent for inflammation detection

  2022cited by this paper
• Optimization, purification, and structure elucidation of Anthraquinone pigment derivative from Talaromyces purpureogenus as a novel promising antioxidant, anticancer, and kidney radio-imaging agentAnthraquinone pigment as a promising antioxidant, anticancer, and radio-imaging agent.

  2022cited by this paper
• Bacteriophages and Food Production: Biocontrol and Bio-Preservation Options for Food Safety

  2022cited by this paper
• Enhancing the Stability of Bacteriophages Using Physical, Chemical, and Nano-Based Approaches: A Review

  2022cited by this paper
• Interactions between bacterial and phage communities in natural environments

  2021cited by this paper
• Radiolabeling, characterization, and preclinical evaluation of plazomicin: A potential tracer for bacterial infection

  2021cited by this paper
• Molecular identification and evaluation of gamma irradiation effect on modulating heavy metals tolerance in some of novel endophytic fungal strains

  2021cited by this paper
• Molecular docking and preliminary bioevaluation of 99mTc-Thiadiazuron as a novel potential agent for cervical cancer imaging

  2020cited by this paper
• A Hundred Years of Bacteriophages: Can Phages Replace Antibiotics in Agriculture and Aquaculture?

  2020cited by this paper
• Recombinant human insulin as a solid tumor potential imaging agent: Radio-synthesis and biological evaluation

  2019cited by this paper
• Isolation, characterization and analysis of bacteriophages from the haloalkaline lake Elmenteita, Kenya

  2019cited by this paper
• Molecular identification and pathogenicity of Ralstonia solanacearum isolates collected from north west of Pakistan

  2019cited by this paper
• "Tau protein targeting via radioiodinated Azure A for brain theranostics: radiolabeling, molecular docking, in vitro and in vivo biological evaluation".

  2019cited by this paper
• Characterization of Bacteriophages Infecting Clinical Isolates of Clostridium difficile

  2018cited by this paper
• Biotechnological applications of bacteriophages: State of the art.

  2018cited by this paper
• Bacteriophages and Bacterial Plant Diseases

  2017cited by this paper
• Biocontrol of Foodborne Salmonella Using Bacteriophages

  2017cited by this paper
• Phylotype analysis of Ralstonia solanacearum strains causing potato bacterial wilt in Karnataka in India

  2014cited by this paper
• Phage therapy for plant disease control.

  2010cited by this paper
• Synthesis of the Human Insulin Gene: Protein Expression, Scaling Up and Bioactivity

  2007cited by this paper
• Identification of crop and weed hosts of Ralstonia solanacearum biovar 2 in the hills of Nepal

  2000cited by this paper
• Detection of Ralstonia solanacearum, Which Causes Brown Rot of Potato, by Fluorescent In Situ Hybridization with 23S rRNA-Targeted Probes

  1998cited by this paper
• Sensitivity of different methods for the detection of Ralstonia solanacearum in potato tuber extracts

  1996cited by this paper
• A detection method for Pseudomonas solanacearum in symptomless potato tubers and some data on its sensitivity and specificity.

  1988cited by this paper
• Biological control of bacterial wilt of potatoes : Attempts to induce resistance by treating tubers with bacteria

  1983cited by this paper
• Uptake of bacteriophage f2 through plant roots

  1982cited by this paper
• An accurate measurement of the catalase crystal period and its use as an internal marker for electron microscopy.

  1967cited by this paper
• The relationship of pathogenicity of Pseudomonas solanacearum to colony appearance in a tetrazolium medium.

  1954cited by this paper

• No citing papers are available for this paper.