Aspergillus oryzae strains heterogeneously produce plant polysaccharide degradation-related enzymes.

Shimma Fujiwa,Ryousuke Kataoka,Kazuhiro Iwashita,Tomohiko Matsuzawa

Published 2025 in Journal of Bioscience and Bioengineering

ABSTRACT

Aspergillus oryzae is used for brewing, and many strains with different brewing characteristics have been isolated. We compared enzymatic activities of eight A. oryzae strains, RIB40, 128, 143, 163, 301, 915, 1108, and 1178 strains, toward plant polysaccharides. The plant polysaccharide degradation-related enzymes produced by A. oryzae change depending on the monosaccharides and polysaccharides added to the culture medium. RIB915 produced more cellulolytic and hemicellulolytic enzymes, whereas RIB40, 128, and 301 produced less of these enzymes than the other strains. In addition, A. oryzae RIB128 produced different glycoside hydrolases in response to monosaccharides and polysaccharides compared with other strains. These results indicated that there is diversity in the production of plant polysaccharide degradation-related enzymes within A. oryzae species.

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