Analysis of specific RNA in cultured cells through quantitative integration of q-PCR and N-SIM single cell FISH images: Application to hormonal stimulation of StAR transcription.

No abstract is available for this paper.

• Publication year

  2016

• Venue

  Molecular and Cellular Endocrinology

• Publication date

  2016-07-05

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/j.mce.2016.04.001PMID 27091298PMCID PMC6181224
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Novel Targets for the Transcription Factors MEF2 in MA-10 Leydig Cells1

  2015influential reference
• Stimulation of StAR expression by cAMP is controlled by inhibition of highly inducible SIK1 via CRTC2, a co-activator of CREB.

  2015cited by this paper
• Mammalian NET-Seq Reveals Genome-wide Nascent Transcription Coupled to RNA Processing

  2015cited by this paper
• Mitochondria-associated Endoplasmic Reticulum Membrane (MAM) Regulates Steroidogenic Activity via Steroidogenic Acute Regulatory Protein (StAR)-Voltage-dependent Anion Channel 2 (VDAC2) Interaction*

  2014influential reference
• Mitochondrial Fusion and ERK Activity Regulate Steroidogenic Acute Regulatory Protein Localization in Mitochondria

  2014cited by this paper
• Three-dimensional super-resolution microscopy of the inactive X chromosome territory reveals a collapse of its active nuclear compartment harboring distinct Xist RNA foci

  2014cited by this paper
• RNAstructure: web servers for RNA secondary structure prediction and analysis

  2013cited by this paper
• Single chromosome transcriptional profiling reveals chromosome-level regulation of gene expression

  2013cited by this paper
• FISH-quant: automatic counting of transcripts in 3D FISH images

  2013cited by this paper
• Erratum: Critical assessment of automated flow cytometry data analysis techniques

  2013cited by this paper
• Neuronal mRNAs travel singly into dendrites

  2012cited by this paper
• Mitochondrial Fusion Is Essential for Steroid Biosynthesis

  2012cited by this paper
• Single cell systems biology by super-resolution imaging and combinatorial labeling

  2012cited by this paper
• cAMP stimulation of StAR expression and cholesterol metabolism is modulated by co-expression of labile suppressors of transcription and mRNA turnover.

  2011cited by this paper
• Cell-to-cell variability of alternative RNA splicing

  2011cited by this paper
• The involvement of specific PKC isoenzymes in phorbol ester-mediated regulation of steroidogenic acute regulatory protein expression and steroid synthesis in mouse Leydig cells.

  2011cited by this paper
• Phosphorylation of the CREB-specific coactivator TORC2 at Ser(307) regulates its intracellular localization in COS-7 cells and in the mouse liver.

  2010cited by this paper
• Detection of individual endogenous RNA transcripts in situ using multiple singly labeled probes.

  2010cited by this paper
• RNAstructure: software for RNA secondary structure prediction and analysis

  2010cited by this paper
• Inactivation of HDAC5 by SIK1 in AICAR-treated C2C12 myoblasts.

  2009cited by this paper
• Pre-mRNA processing reaches back to transcription and ahead to translation.

  2009cited by this paper
• cAMP-dependent posttranscriptional regulation of steroidogenic acute regulatory (STAR) protein by the zinc finger protein ZFP36L1/TIS11b.

  2009cited by this paper
• Imaging individual mRNA molecules using multiple singly labeled probes

  2008cited by this paper
• Mitochondrial A-Kinase Anchoring Protein 121 Binds Type II Protein Kinase A and Enhances Steroidogenic Acute Regulatory Protein-Mediated Steroidogenesis in MA-10 Mouse Leydig Tumor Cells1

  2008cited by this paper
• Towards many colors in FISH on 3D-preserved interphase nuclei

  2006cited by this paper
• Absolute and relative QPCR quantification of plasmid copy number in Escherichia coli.

  2006cited by this paper
• Rules of engagement: co-transcriptional recruitment of pre-mRNA processing factors.

  2005cited by this paper
• Cyclic adenosine 3',5'-monophosphate (cAMP) enhances cAMP-responsive element binding (CREB) protein phosphorylation and phospho-CREB interaction with the mouse steroidogenic acute regulatory protein gene promoter.

  2005cited by this paper
• Temporal and spatial changes in transcription factor binding and histone modifications at the steroidogenic acute regulatory protein (stAR) locus associated with stAR transcription.

  2004cited by this paper
• The CREB coactivator TORC2 functions as a calcium- and cAMP-sensitive coincidence detector.

  2004cited by this paper
• An extensive network of coupling among gene expression machines

  2002cited by this paper
• Interaction between arachidonic acid and cAMP signaling pathways enhances steroidogenesis and StAR gene expression in MA-10 Leydig tumor cells.

  2002cited by this paper
• PBR, StAR, AND PKA: PARTNERS IN CHOLESTEROL TRANSPORT IN STEROIDOGENIC CELLS

  2002cited by this paper
• Mitochondrial Processing of Newly Synthesized Steroidogenic Acute Regulatory Protein (StAR), but Not Total StAR, Mediates Cholesterol Transfer to Cytochrome P450 Side Chain Cleavage Enzyme in Adrenal Cells*

  2001cited by this paper
• RNA polymerase II and the integration of nuclear events.

  2000cited by this paper
• GATA-1 and GATA-4 transactivate inhibin/activin beta-B-subunit gene transcription in testicular cells.

  2000cited by this paper
• SF-1 (Steroidogenic Factor-1) and C/EBPβ (CCAAT/Enhancer Binding Protein-β) Cooperate to Regulate the Murine StAR (Steroidogenic Acute Regulatory) Promoter

  1999cited by this paper
• SF-1 (steroidogenic factor-1) and C/EBP beta (CCAAT/enhancer binding protein-beta) cooperate to regulate the murine StAR (steroidogenic acute regulatory) promoter.

  1999cited by this paper
• Targeted disruption of the mouse gene encoding steroidogenic acute regulatory protein provides insights into congenital lipoid adrenal hyperplasia.

  1997cited by this paper
• Expression of steroidogenic acute regulatory protein (StAR) in the human ovary.

  1996cited by this paper
• Differential effects of dimethylsulfoxide on steroidogenesis in mouse MA-10 and rat R2C Leydig tumor cells.

  1995cited by this paper
• Role of steroidogenic acute regulatory protein in adrenal and gonadal steroidogenesis.

  1995cited by this paper
• The purification, cloning, and expression of a novel luteinizing hormone-induced mitochondrial protein in MA-10 mouse Leydig tumor cells. Characterization of the steroidogenic acute regulatory protein (StAR).

  1994influential reference
• Glucocorticoid and cyclic adenosine 3'5'-monophosphate-mediated induction of cholesterol side-chain cleavage cytochrome P450 (P450scc) in MA-10 tumor Leydig cells. Increases in mRNA are cycloheximide sensitive.

  1990cited by this paper
• Adrenocortical atrophy of hypophysectomized rats can be reduced by corticotropin-releasing hormone (CRH)

  1990cited by this paper
• Characterization of several clonal lines of cultured Leydig tumor cells: gonadotropin receptors and steroidogenic responses.

  1981cited by this paper

• Single-cell resolution uncovers neighboring cell subtypes that share steroidogenic capacity during fetal testis development

  2025cites this paper
• Distinctive functioning of STARD1 in the fetal Leydig cells compared to adult Leydig and adrenal cells. Impact of Hedgehog signaling via the primary cilium.

  2021cites this paper
• Activation and expression of endogenous CREB‐regulated transcription coactivators (CRTC) 1, 2 and 3 in the rat adrenal gland

  2020cites this paper
• A global lipid map defines a network essential for Zika virus replication

  2020cites this paper
• GLI3 resides at the intersection of hedgehog and androgen action to promote male sex differentiation

  2020influential citation
• Quantification of Cadophora luteo-olivacea From Grapevine Nursery Stock and Vineyard Soil Using Droplet Digital PCR.

  2020cites this paper
• STARD1 Functions in Mitochondrial Cholesterol Metabolism and Nascent HDL Formation. Gene Expression and Molecular mRNA Imaging Show Novel Splicing and a 1:1 Mitochondrial Association

  2020cites this paper
• Droplet Digital PCR Technology for Detection of Ilyonectria liriodendri from Grapevine Environmental Samples.

  2020cites this paper
• StAR, a bridge from ApoE, LDL, and HDL cholesterol trafficking to mitochondrial metabolism

  2019cites this paper
• Injection molded open microfluidic well plate inserts for user-friendly coculture and microscopy

  2019cites this paper
• Cholesterol signaling in single cells: lessons from STAR and sm-FISH.

  2018cites this paper
• Molecular Methods to Detect and Quantify Botryosphaeriaceae Inocula Associated With Grapevine Dieback in Australia.

  2018cites this paper
• Microfluidic devices for single-cell and organ-level studies

  2017cites this paper
• Monitoring of Dual CRISPR/Cas9-Mediated Steroidogenic Acute Regulatory Protein Gene Deletion and Cholesterol Accumulation Using High-Resolution Fluorescence In Situ Hybridization in a Single Cell

  2017cites this paper
• A single cell level measurement of StAR expression and activity in adrenal cells.

  2017influential citation
• Regulation of StAR by the N-terminal Domain and Coinduction of SIK1 and TIS11b/Znf36l1 in Single Cells

  2016cites this paper
• ACTH Action on StAR Biology

  2016cites this paper