Production, biological characterization, and applications of natural pigments from filamentous fungi.

Natural dyes are gaining prominence as safer, bioactive alternatives to synthetic colorants, with microbial production offering efficient and cost-effective methods. This study isolated and evaluated pigment-producing fungi from Saudi Arabia's Eastern Province, identifying Sabouraud Dextrose Agar (SDA) as optimal for initial screening. While ten high-yield strains were tested in liquid fermentation and immobilization, solid-state fermentation yielded superior pigment production. Genetic sequencing identified the top five strains as Aspergillus terreus (PQ134479), Fusarium solani (PQ134082), Achaetomium cristalliferum (PQ134101), Chaetomium strumarium (PQ134105) and Aspergillus terreus (PQ133589), Water proved to be the best extraction solvent. Optimization of culture conditions revealed temperature and pH as critical factors for pigment yield. Antimicrobial assays showed promising inhibitory effects against two multidrug resistance strains which are Staphylococcus aureus MRSA (PQ097594) and Escherichia coli ESBL (PQ097597), ABTS assays indicated strong, dose-dependent antioxidant activity, particularly for C. strumarium. Acute toxicity tests on Daphnia magna confirmed the pigments' non-toxic nature. Successful textile dyeing on cotton and wool was achieved, with alum showing the best color fixation. Food applications in rice and dough demonstrated good thermal stability and color retention. These findings underscore the potential of fungal pigments as sustainable, non-toxic alternatives for industrial applications, particularly in the food and textile sectors.

• Publication year

  2025

• Venue

  Brazilian journal of biology = Revista brasleira de biologia

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1590/1519-6984.298448PMID 41221943
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Methods for screening and evaluation of antimicrobial activity: A review of protocols, advantages, and limitations

  2024cited by this paper
• Application and Stability of Fungal Pigments using Jelly‐Sweets as a Food Model System

  2023cited by this paper
• Production of Pigments under Submerged Culture through Repeated Batch Fermentation of Immobilized Talaromyces atroroseus GH2

  2023cited by this paper
• Improvement of the Pour Plate Method by Separate Sterilization of Agar and Other Medium Components and Reduction of the Agar Concentration

  2023cited by this paper
• Exploring colorant production by amazonian filamentous fungi: Stability and applications

  2023cited by this paper
• Effects of textile dyeing effluent on the environment and its treatment: A review

  2022cited by this paper
• Antioxidant, Antibacterial and Dyeing Potential of Crude Pigment Extract of Gonatophragmium triuniae and Its Chemical Characterization

  2022cited by this paper
• Orange-Brown Pigment Production from an Endophytic Fungus Aspergillus Sp. N11 and its Potential Pharmaceutical Applications

  2022cited by this paper
• Colorful Treasure From Agro-Industrial Wastes: A Sustainable Chassis for Microbial Pigment Production

  2022cited by this paper
• Isolation and Identification of Natural Colorant Producing Soil-Borne Aspergillus niger from Bangladesh and Extraction of the Pigment

  2021cited by this paper
• Interactive Tree Of Life (iTOL) v5: an online tool for phylogenetic tree display and annotation

  2021cited by this paper
• The Realm of Microbial Pigments in the Food Color Market

  2021cited by this paper
• Colors of life: a review on fungal pigments

  2021cited by this paper
• Production of Pigments by Filamentous Fungi Cultured on Agro-Industrial by-Products Using Submerged and Solid-State Fermentation Methods

  2021cited by this paper
• Safety Evaluation of Fungal Pigments for Food Applications

  2021cited by this paper
• Analysis of culturable airborne fungi in outdoor environments in Tianjin, China

  2021cited by this paper
• Highlighting the Crude Oil Bioremediation Potential of Marine Fungi Isolated from the Port of Oran (Algeria)

  2020cited by this paper
• Fungi as a Potential Source of Pigments: Harnessing Filamentous Fungi

  2020cited by this paper
• Cheese whey as a potential substrate for Monascus pigments production

  2020cited by this paper
• Production of Bio-Based Pigments from Food Processing Industry By-Products (Apple, Pomegranate, Black Carrot, Red Beet Pulps) Using Aspergillus carbonarius

  2020cited by this paper
• Natural Pigments of Microbial Origin

  2020cited by this paper
• Effective Molecular Identification of Ectomycorrhizal Fungi: Revisiting DNA Isolation Methods

  2019cited by this paper
• Evaluation of in vitro antioxidant activity of fungal pigments

  2019cited by this paper
• Multifaceted Applications of Microbial Pigments: Current Knowledge, Challenges and Future Directions for Public Health Implications

  2019cited by this paper
• Chlorinated Azaphilone Pigments with Antimicrobial and Cytotoxic Activities Isolated from the Deep Sea Derived Fungus Chaetomium sp. NA-S01-R1

  2018cited by this paper
• Applications of fungal pigments in Biotechnology

  2018cited by this paper
• Brown pigment production by Penicillium. Purpurogenum using Moringa oleifera leaves and identification of phytochemicals by analytical techniques

  2018cited by this paper
• Optimisation and characterisation of the orange pigment produced by a cold adapted strain of Penicillium sp. (GBPI_P155) isolated from mountain ecosystem

  2018cited by this paper
• Production of red pigments by Monascus ruber CCT 3802 using lactose as a substrate

  2017cited by this paper
• Filamentous ascomycetes fungi as a source of natural pigments

  2017cited by this paper
• Natural Colorants: Food Colorants from Natural Sources.

  2017cited by this paper
• Antimicrobial Activity of Pigments Extracted from Rhodotorula glutinis Against Some Bacteria and Fungi

  2016cited by this paper
• Natural colorants from filamentous fungi

  2016cited by this paper
• Food colorants: Challenges, opportunities and current desires of agro-industries to ensure consumer expectations and regulatory practices

  2016cited by this paper
• Review : Potential production of carotenoids from Neurospora

  2016cited by this paper
• Methods for in vitro evaluating antimicrobial activity: A review☆

  2015cited by this paper
• Reddish brown pigments from Alternaria alternata for textile dyeing and printing

  2015cited by this paper
• Physiological Studies on the Monascus ruber Red Pigment and GC/MS Analysis

  2015cited by this paper
• Investigating the influence of pH, temperature and agitation speed on yellow pigment production by Penicillium aculeatum ATCC 10409

  2015cited by this paper
• Biological roles of fungal carotenoids

  2015cited by this paper
• Isolation and molecular identification of deteriorating fungi from Cyrus the Great tomb stones

  2014cited by this paper
• Bioprospecting of Amazon soil fungi with the potential for pigment production

  2014cited by this paper
• Purification and characterization of a new red pigment from Monascus purpureus in submerged fermentation

  2011cited by this paper
• Dyeing of Wool Fibers with Natural Fungal Dye from Suillus luteus

  2011cited by this paper
• Is inclusion of Sabouraud dextrose agar essential for the laboratory diagnosis of fungal keratitis?

  2010cited by this paper
• Fungal polyketide azaphilone pigments as future natural food colorants?

  2010cited by this paper
• Improved Production of Pharmacologically-active Sclerotiorin by Penicillium sclerotiorum

  2010cited by this paper
• Photostability of natural orange-red and yellow fungal pigments in liquid food model systems.

  2009cited by this paper
• Dyeing of wool with natural anthraquinone dyes from Fusarium oxysporum

  2007cited by this paper
• Application of solid-state fermentation to food industry—A review

  2006cited by this paper
• Assessment of Bioactivity of Indian Medicinal Plants Using Brine Shrimp (Artemia salina) Lethality Assay

  2005cited by this paper
• Biopigments from Monascus: strains selection, citrinin production and color stability

  2005cited by this paper
• Molecular Identification of Ectomycorrhizal Mycelium in Soil Horizons

  2003cited by this paper
• Relationship between Secondary Metabolism and Fungal Development

  2002cited by this paper
• Detection and Identification of Fungal Pathogens by PCR and by ITS2 and 5.8S Ribosomal DNA Typing in Ocular Infections

  2001cited by this paper
• Acute toxicity test with Daphnia magna: an alternative to mammals in the prescreening of chemical toxicity?

  2000cited by this paper
• Kinetic analysis of red pigment and citrinin production by Monascus ruber as a function of organic acid accumulation.

  2000cited by this paper
• Antioxidant activity applying an improved ABTS radical cation decolorization assay.

  1999cited by this paper
• Analysis of Fungal Diversity in the Wheat Rhizosphere by Sequencing of Cloned PCR-Amplified Genes Encoding 18S rRNA and Temperature Gradient Gel Electrophoresis

  1999cited by this paper
• Microbial Cell Factories Identification of Potentially Safe Promising Fungal Cell Factories for the Production of Polyketide Natural Food Colorants Using Chemotaxonomic Rationale

  year unknowncited by this paper

• No citing papers are available for this paper.