Exploring reductive stress as a regulation approach for Pseudomonas Aeruginosa biofilm

Summary Pseudomonas aeruginosa (P. aeruginosa) biofilms pose substantial challenges in clinical settings due to their resistance to conventional antibiotic treatments. This study investigated the influence of reductive stress on the formation and eradication of P. aeruginosa biofilms. A series of redox-active compounds were employed to assess their impact on both biofilm development and disruption. Among them, hydrazine (HZ) showed potent activity. Mechanistic studies revealed that these compounds suppressed pyocyanin production and impaired bacterial metabolic activity. A structure-activity relationship analysis highlighted hydroxylamine (HA) as a promising candidate, owing to its favorable balance between efficacy and cytotoxicity. Notably, quantitative reverse transcription polymerase chain reaction (RT-qPCR) analysis of HA-treated P. aeruginosa suggested inhibition of matrix biosynthesis, quorum sensing, and oxidative stress defenses. This study provides insights into biofilm management by targeting the redox environment, offering potential strategies for the development of redox-based therapies to combat biofilm-associated infections.

• Publication year

  2025

• Venue

  iScience

• Publication date

  2025-11-01

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1016/j.isci.2025.113973PMID 41362616PMCID 12682017
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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