Characterisation of four novel bacteriophages targeting carbapenem-resistant Klebsiella pneumoniae and their lytic activity alone and in combination

Highlights • Four newly isolated lytic phages showed no antibiotic resistance or virulence genes.• Phages demonstrated rapid adsorption, short latent period and variable burst size.• Phages were active against carbapenemase-producing Klebsiella pneumoniae strains.• Phage cocktail enhanced lytic activity compared to individual phages.• The antibiofilm activity suggests potential use in biofilm-related infection.

• Publication year

  2025

• Venue

  Current Research in Microbial Sciences

• Publication date

  2025-11-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/j.crmicr.2025.100509PMID 41333671PMCID 12666361
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The LAMP-CRISPR-Cas13a technique for detecting the CBASS mechanism of phage resistance in bacteria

  2025cited by this paper
• Phage therapy for Klebsiella pneumoniae: Understanding bacteria–phage interactions for therapeutic innovations

  2025cited by this paper
• Characterization of four novel bacteriophages targeting multi-drug resistant Klebsiella pneumoniae strains of sequence type 147 and 307

  2024cited by this paper
• Personalized bacteriophage therapy outcomes for 100 consecutive cases: a multicentre, multinational, retrospective observational study

  2024cited by this paper
• A Klebsiella-phage cocktail to broaden the host range and delay bacteriophage resistance both in vitro and in vivo

  2024cited by this paper
• Current status of bacteriophage therapy for severe bacterial infections

  2024cited by this paper
• Targeted phage hunting to specific Klebsiella pneumoniae clinical isolates is an efficient antibiotic resistance and infection control strategy

  2024cited by this paper
• Therapeutic Potential of Intravenous Phage as Standalone Therapy for Recurrent Drug-Resistant Urinary Tract Infections

  2023cited by this paper
• The Virtuous Galleria mellonella Model for Scientific Experimentation

  2023cited by this paper
• Isolation, Characterization, Genome Analysis and Host Resistance Development of Two Novel Lastavirus Phages Active against Pandrug-Resistant Klebsiella pneumoniae

  2023cited by this paper
• Bacteriophage T4 Head: Structure, Assembly, and Genome Packaging

  2023cited by this paper
• Application of Phage Therapy in a Case of a Chronic Hip-Prosthetic Joint Infection due to Pseudomonas aeruginosa: An Italian Real-Life Experience and In Vitro Analysis

  2023cited by this paper
• Phage therapy: From biological mechanisms to future directions.

  2023cited by this paper
• Characterization and genome analysis of phage vB_KpnS_SXFY507 against Klebsiella pneumoniae and efficacy assessment in Galleria mellonella larvae

  2023cited by this paper
• Bacteriophage therapy against ESKAPE bacterial pathogens: Current status, strategies, challenges, and future scope.

  2023cited by this paper
• Designing phage cocktails to combat the emergence of bacteriophage-resistant mutants in multidrug-resistant Klebsiella pneumoniae

  2023cited by this paper
• Phages for treatment of Klebsiella pneumoniae infections.

  2023cited by this paper
• Bacteriophages with depolymerase activity in the control of antibiotic resistant Klebsiella pneumoniae biofilms

  2023cited by this paper
• Characterization of bacteriophage BUCT631 lytic for K1 Klebsiella pneumoniae and its therapeutic efficacy in Galleria mellonella larvae

  2023cited by this paper
• A K-17 serotype specific Klebsiella phage JKP2 with biofilm reduction potential

  2023cited by this paper
• Resistome and virulome accretion in an NDM-1-producing ST147 sublineage of Klebsiella pneumoniae associated with an outbreak in Tuscany, Italy: a genotypic and phenotypic characterisation.

  2022cited by this paper
• Phage-Based Control of Methicillin Resistant Staphylococcus aureus in a Galleria mellonella Model of Implant-Associated Infection

  2022cited by this paper
• Klebsiella pneumoniae Biofilms and Their Role in Disease Pathogenesis

  2022cited by this paper
• Combination of pre-adapted bacteriophage therapy and antibiotics for treatment of fracture-related infection due to pandrug-resistant Klebsiella pneumoniae

  2022cited by this paper
• Animal Models of Phage Therapy

  2021cited by this paper
• Galleria mellonella as a Suitable Model of Bacterial Infection: Past, Present and Future

  2021cited by this paper
• Phage Cocktail Development for Bacteriophage Therapy: Toward Improving Spectrum of Activity Breadth and Depth

  2021cited by this paper
• Phage Therapy for Antibiotic-Resistant Bacterial Infections.

  2021cited by this paper
• A Roadmap for Genome-Based Phage Taxonomy

  2021cited by this paper
• The Changing Face of the Family Enterobacteriaceae (Order: “Enterobacterales”): New Members, Taxonomic Issues, Geographic Expansion, and New Diseases and Disease Syndromes

  2021cited by this paper
• Isolation and Characterization of Klebsiella Phages for Phage Therapy

  2021cited by this paper
• GenBank

  2020cited by this paper
• Eradication of a multi-drug resistant, carbapenemase-producing Klebsiella pneumoniae isolate following oral and intra-rectal therapy with a custom-made, lytic bacteriophage preparation.

  2020cited by this paper
• Isolation and Characterization of a Novel Bacteriophage Infecting Carbapenem-Resistant Klebsiella pneumoniae

  2020cited by this paper
• Population genomics of Klebsiella pneumoniae

  2020cited by this paper
• Prolonged outbreak of New Delhi metallo-beta-lactamase-producing carbapenem-resistant Enterobacterales (NDM-CRE), Tuscany, Italy, 2018 to 2019

  2020influential reference
• Isolation and Characterization of Two Klebsiella pneumoniae Phages Encoding Divergent Depolymerases

  2020cited by this paper
• Bacteriophage Therapy for Critical Infections Related to Cardiothoracic Surgery

  2020cited by this paper
• VIRIDIC—A Novel Tool to Calculate the Intergenomic Similarities of Prokaryote-Infecting Viruses

  2020cited by this paper
• Phage Therapy for Limb-threatening Prosthetic Knee Klebsiella pneumoniae Infection: Case Report and In Vitro Characterization of Anti-biofilm Activity.

  2020cited by this paper
• The Characteristic of Virulence, Biofilm and Antibiotic Resistance of Klebsiella pneumoniae

  2020cited by this paper
• ResFinder 4.0 for predictions of phenotypes from genotypes

  2020cited by this paper
• New Bacteriophages against Emerging Lineages ST23 and ST258 of Klebsiella pneumoniae and Efficacy Assessment in Galleria mellonella Larvae

  2019cited by this paper
• Type I-F CRISPR-Cas resistance against virulent phages results in abortive infection and provides population-level immunity

  2019cited by this paper
• The Removal of Endo- and Enterotoxins From Bacteriophage Preparations

  2019cited by this paper
• Phage Lysis: Multiple Genes for Multiple Barriers.

  2019cited by this paper
• Bacteriophages of Klebsiella spp., their diversity and potential therapeutic uses

  2019cited by this paper
• VFDB 2019: a comparative pathogenomic platform with an interactive web interface

  2018cited by this paper
• Design of a Broad-Range Bacteriophage Cocktail That Reduces Pseudomonas aeruginosa Biofilms and Treats Acute Infections in Two Animal Models

  2018cited by this paper
• Practical Advice on the One-Step Growth Curve.

  2018cited by this paper
• Colonization, Infection, and the Accessory Genome of Klebsiella pneumoniae

  2018cited by this paper
• Characterization of CRISPR-Cas Systems in Clinical Klebsiella pneumoniae Isolates Uncovers Its Potential Association With Antibiotic Susceptibility

  2018cited by this paper
• ViPTree: the viral proteomic tree server

  2017influential reference
• Comparative analysis of CRISPR‐Cas systems in Klebsiella genomes

  2017cited by this paper
• A comparative study of different strategies for removal of endotoxins from bacteriophage preparations.

  2017cited by this paper
• φBO1E, a newly discovered lytic bacteriophage targeting carbapenemase-producing Klebsiella pneumoniae of the pandemic Clonal Group 258 clade II lineage

  2017influential reference
• Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads

  2016influential reference
• CARD 2017: expansion and model-centric curation of the comprehensive antibiotic resistance database

  2016cited by this paper
• Genome-based phylogeny and taxonomy of the 'Enterobacteriales': proposal for Enterobacterales ord. nov. divided into the families Enterobacteriaceae, Erwiniaceae fam. nov., Pectobacteriaceae fam. nov., Yersiniaceae fam. nov., Hafniaceae fam. nov., Morganellaceae fam. nov., and Budviciaceae fam. nov.

  2016cited by this paper
• Galleria mellonella infection models for the study of bacterial diseases and for antimicrobial drug testing

  2016cited by this paper
• Software-based analysis of bacteriophage genomes, physical ends, and packaging strategies

  2016cited by this paper
• Improvements to PATRIC, the all-bacterial Bioinformatics Database and Analysis Resource Center

  2016influential reference
• Klebsiella pneumoniae: Going on the Offense with a Strong Defense

  2016cited by this paper
• RASTtk: A modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes

  2015cited by this paper
• Isolation of Phages for Phage Therapy: A Comparison of Spot Tests and Efficiency of Plating Analyses for Determination of Host Range and Efficacy

  2015cited by this paper
• Structural remodeling of bacteriophage T4 and host membranes during infection initiation

  2015cited by this paper
• Resistance Determinants and Mobile Genetic Elements of an NDM-1-Encoding Klebsiella pneumoniae Strain

  2014influential reference
• Contractile tail machines of bacteriophages.

  2012cited by this paper
• Fiji: an open-source platform for biological-image analysis

  2012cited by this paper
• Lysis from without

  2011cited by this paper
• Protein structure prediction on the Web: a case study using the Phyre server

  2009cited by this paper
• Phage host range and efficiency of plating.

  2009cited by this paper
• Practical methods for determining phage growth parameters.

  2009cited by this paper
• Protein database searches using compositionally adjusted substitution matrices

  2005cited by this paper
• Structure of the cell-puncturing device of bacteriophage T4

  2002cited by this paper
• KpnAI, a new type I restriction-modification system in Klebsiella pneumoniae.

  1997cited by this paper
• Basic local alignment search tool.

  1990cited by this paper
• Isolation of Bacteriophages

  1962cited by this paper
• Therapy

  1939cited by this paper

• A comprehensive analysis of the kinetics of infection of lytic bacteriophages specific to the ESKAPE and critical pathogens

  2026cites this paper