Gut microbiome enterotypes and temporal variations during a six-month inulin-supplemented weight loss randomized controlled trial in Thai children with obesity

Gut microbiome profiles can reflect the host’s health status and have the potential to predict the effectiveness of medical interventions. In this study, gut microbiome enterotypes of 143 Thai children with obesity who participated in a randomized controlled trial of inulin supplementation were analyzed to identify microbial markers for weight loss responses and healthier lifestyles. Two gut microbiome enterotypes, which encompass 25 % of the children with obesity, were exclusive to children with obesity, including a rarely described Megasphaera enterotype. Megasphaera enterotype was also positively associated with dietary fiber intake specifically in the inulin group. Prevotella and Bacteroides enterotypes are significantly associated with poor health status, including longer times spent on electronic devices and lower concentrations of butyrate, a beneficial short-chain fatty acid. Children with these enterotypes also exhibited some of the best weight loss outcomes after receiving only behavioral and dietary advice. Observed associations between enterotypes and clinical parameters are consistent with earlier research of different populations. Intervention-specific associations suggest potential criteria for a personalized selection of weight loss interventions.

• Publication year

  2025

• Venue

  Computational and Structural Biotechnology Journal

• Publication date

  2025-11-01

• Fields of study

  Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.csbj.2025.11.021PMID 41321998PMCID 12661350
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Integration of 168,000 samples reveals global patterns of the human gut microbiome.

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• The interplay between diet and the gut microbiome: implications for health and disease

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• Enterosignatures define common bacterial guilds in the human gut microbiome.

  2023influential reference
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• Recent insights into the role of microbiome in the pathogenesis of obesity

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• Obesity: Epidemiology, Pathophysiology, and Therapeutics

  2021cited by this paper
• Innate immunity and inflammophagy: balancing the defence and immune homeostasis

  2021cited by this paper
• Temporal variability in quantitative human gut microbiome profiles and implications for clinical research

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• pyComBat, a Python tool for batch effects correction in high-throughput molecular data using empirical Bayes methods

  2020cited by this paper
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  2020cited by this paper
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  2020cited by this paper
• PICRUSt2 for prediction of metagenome functions

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  2020cited by this paper
• Impact of early events and lifestyle on the gut microbiota and metabolic phenotypes in young school-age children

  2019cited by this paper
• The gut microbiome of Mexican children affected by obesity.

  2019cited by this paper
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  2019cited by this paper
• Obesity paradox in cardiovascular disease: where do we stand?

  2019cited by this paper
• Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2

  2019influential reference
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  2019cited by this paper
• Timescales of gut microbiome dynamics.

  2019cited by this paper
• The human gut microbiota: Metabolism and perspective in obesity

  2018cited by this paper
• Optimizing taxonomic classification of marker-gene amplicon sequences with QIIME 2’s q2-feature-classifier plugin

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• Childhood Obesity and Firmicutes/Bacteroidetes Ratio in the Gut Microbiota: A Systematic Review

  2018cited by this paper
• Urban Diets Linked to Gut Microbiome and Metabolome Alterations in Children: A Comparative Cross-Sectional Study in Thailand

  2018influential reference
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  2018cited by this paper
• Health Effects of Overweight and Obesity in 195 Countries over 25 Years.

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• Diversity and enterotype in gut bacterial community of adults in Taiwan

  2017cited by this paper
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  2016cited by this paper
• Microbial Community of Healthy Thai Vegetarians and Non-Vegetarians, Their Core Gut Microbiota, and Pathogen Risk.

  2016cited by this paper
• Obesity and Risk of Cancer: An Introductory Overview.

  2016cited by this paper
• DADA2: High resolution sample inference from Illumina amplicon data

  2016cited by this paper
• Enhanced butyrate formation by cross-feeding between Faecalibacterium prausnitzii and Bifidobacterium adolescentis.

  2015cited by this paper
• Distinct gut microbiota of healthy children from two different geographic regions of Thailand

  2015cited by this paper
• Diversity in gut bacterial community of school-age children in Asia

  2015cited by this paper
• A Taxonomic Signature of Obesity in the Microbiome? Getting to the Guts of the Matter

  2014cited by this paper
• Meta‐analyses of human gut microbes associated with obesity and IBD

  2014cited by this paper
• Gut microbiota and energy balance: role in obesity

  2014cited by this paper
• Obesity as a Major Risk Factor for Cancer

  2013cited by this paper
• Gut Microbiota from Twins Discordant for Obesity Modulate Metabolism in Mice

  2013cited by this paper
• Intestinal Dysbiosis and Depletion of Butyrogenic Bacteria in Clostridium difficile Infection and Nosocomial Diarrhea

  2013cited by this paper
• Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies

  2012cited by this paper
• Enterotypes of the human gut microbiome

  2011influential reference
• Linking Long-Term Dietary Patterns with Gut Microbial Enterotypes

  2011cited by this paper
• Microbial ecology: Human gut microbes associated with obesity

  2006cited by this paper
• The gut microbiota as an environmental factor that regulates fat storage.

  2004cited by this paper
• Use and abuse of HOMA modeling.

  2004influential reference
• Multifactorial causation of obesity: implications for prevention.

  1998cited by this paper
• Dietary fiber.

  1976cited by this paper

• No citing papers are available for this paper.