Genetic Diversity of Tuberose (Polianthes tuberosa L.) Germplasm through Molecular Approaches to Obtain Desirable Plant Materials for Future Breeding Programs

Vardhelli Soukya,Soumen Maitra,Nandita Sahana,Saikat Das,R. Mandal,Arpita Mandal Khan,Arindam Das,Ashok Choudhury,Prodyut Kumar Paul,Ahmed Gaber,Mohammed M. Althaqaff,A. Hossain

Published 2025 in Phyton

ABSTRACT

: The present study investigated the genetic diversity of 24 germplasms of Polianthes tuberosa L. via 16 inter simple sequence repeat (ISSR) marker techniques. The research findings revealed that the ISSR markers presented higher levels of band reproducibility and were more efficient at clustering germplasms. Among the 16 markers examined in this study, 12 had a complete polymorphism rate of 100%. The molecular analysis revealed a PIC ranging from 0.079 to 0.373, with a mean value of 0.30, whereas the range of the marker index was from 0.0001 to 0.409, with an average value of 0.03, and the primer resolving power ranged from 0.173 to 4.173, with a mean value of 2.02. The UPGMA clustering dendrogram indicated that all 24 germplasms were grouped into three main clusters. The study revealed a variable range of tree distances between 0.185 and 0.621, with the highest tree distance (0.621) detected between germplasms BR-24 and BR-1. Through these studies, the dissimilarity among the germplasms was evaluated, and diverse parents were identified for further crop improvement programs.

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