BpGRAS1 confers salt tolerance by mediating osmotic potential and reactive oxygen species scavenging capacity in Betula platyphylla.

Plant-specific GRAS transcription factors play crucial roles in regulating responses to abiotic stresses. This study identified and characterized the GRAS protein BpGRAS1 from Betula platyphylla. BpGRAS1 exhibits nuclear localization without transcriptional activation activity and demonstrates responsiveness to salt stress. Through the generation and analysis of BpGRAS1 overexpressing and knockout plants, their salt tolerance was evaluated via enhancement and loss of function studies. Under salt stress conditions, BpGRAS1 overexpression enhanced reactive oxygen species (ROS) scavenging capacity and proline content significantly. This enhancement correlated with increased activities of superoxide dismutase (SOD) and peroxidase (POD). Furthermore, BpGRAS1 facilitates these physiological changes by regulating the expression of SOD, POD and P5CS genes, thus mitigating cellular damage and improving salt tolerance. RNA sequencing analysis identified multiple differentially expressed genes (DEGs), including stress tolerance-related genes and transcription factors potentially regulated by BpGRAS1. Yeast one-hybrid (Y1H) assays, chromatin immunoprecipitation (ChIP), and luciferase (LUC) reporter assays demonstrated that BpbHLH71 functions as an upstream regulator of BpGRAS1, while BpGRAS1 directly regulates BpVND7 and BpCCT1 by binding to GARE (AAACAGA) elements. This research establishes BpGRAS1 as a positive regulator of salt tolerance in B. platyphylla, providing initial insights into the underlying molecular mechanisms and valuable genetic resources.

• Publication year

  2025

• Venue

  International Journal of Biological Macromolecules

• Publication date

  2025-11-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.ijbiomac.2025.149134PMID 41271062
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• bHLH transcription factor from Lilium pumilum, LpbHLH144 confers the salt and alkali stress tolerance of tobacco.

  2025cited by this paper
• GmFER1 , a soybean ferritin, enhances tolerance to salt stress and root rot disease and improves soybean yield

  2025cited by this paper
• The NAC transcription factor PagNAC17 enhances salt tolerance in poplar by alleviating photosynthetic inhibition.

  2025cited by this paper
• Pepper (Capsicum annuum L.) AP2/ERF transcription factor, CaERF2 enhances salt stress tolerance through ROS scavenging

  2025cited by this paper
• A bHLH transcription factor RrUNE12 regulates salt tolerance and promotes ascorbate synthesis

  2025cited by this paper
• StMAPKK1 enhances drought and salt tolerance in potato via ROS scavenging and stomatal closure.

  2025cited by this paper
• The specific HbHAK2 promoter from halophytic Hordeum brevisubulatum regulates root development under salt stress

  2024cited by this paper
• Birch WRKY transcription factor, BpWRKY32, confers salt tolerance by mediating stomatal closing, proline accumulation, and reactive oxygen species scavenging.

  2024cited by this paper
• PagbHLH35 Enhances Salt Tolerance through Improving ROS Scavenging in Transgenic Poplar

  2024cited by this paper
• Identification of WRKY transcription factors in Ipomoea pes-caprae and functional role of IpWRKY16 in sweet potato salt stress response

  2024cited by this paper
• The BpPP2C-BpMADS11-BpERF61 signaling confers drought tolerance in Betula platyphylla.

  2024cited by this paper
• Genome-wide identification of R2R3-MYB transcription factors in Betula platyphylla and functional analysis of BpMYB95 in salt tolerance.

  2024cited by this paper
• Functional analysis of maize GRAS transcription factor gene ZmGRAS72 in response to drought and salt stresses

  2024cited by this paper
• PbGBF3 enhances salt response in pear by upregulating PbAPL2 and PbSDH1 and reducing ABA-mediated salt sensitivity.

  2024cited by this paper
• PagMYB151 facilitates proline accumulation to enhance salt tolerance of poplar

  2023cited by this paper
• Genome-wide identification, characterization, and expression analysis of CCT transcription factors in poplar.

  2023cited by this paper
• The bHLH transcription factor AhbHLH121 improves salt tolerance in peanut.

  2023cited by this paper
• Spatial transcriptomics of a lycophyte root sheds light on root evolution.

  2023cited by this paper
• EbbHLH80 Enhances Salt Responses by Up-Regulating Flavonoid Accumulation and Modulating ROS Levels

  2023cited by this paper
• Overexpression of ThSCL32 confers salt stress tolerance by enhancing ThPHD3 gene expression in Tamarix hispida.

  2023cited by this paper
• Multifaceted roles of GRAS transcription factors in growth and stress responses in plants

  2022cited by this paper
• The R2R3-MYB transcription factor ThRAX2 recognized a new element MYB-T (CTTCCA) to enhance cadmium tolerance in Tamarix hispida.

  2022cited by this paper
• The upstream regulatory mechanism of BplMYB46 and the function of upstream regulatory factors that mediate resistance to stress in Betula platyphylla

  2022cited by this paper
• Tamarix hispida NAC Transcription Factor ThNAC4 Confers Salt and Drought Stress Tolerance to Transgenic Tamarix and Arabidopsis

  2022cited by this paper
• Pepper bHLH transcription factor CabHLH035 contributes to salt tolerance by modulating ion homeostasis and proline biosynthesis

  2022cited by this paper
• Genome-wide study of the GRAS gene family in Hibiscus hamabo Sieb. et Zucc and analysis of HhGRAS14-induced drought and salt stress tolerance in Arabidopsis.

  2022cited by this paper
• A mechanism coordinating root elongation, endodermal differentiation, redox homeostasis and response.

  2021cited by this paper
• GRAS-domain transcription factor PAT1 regulates jasmonic acid biosynthesis in grape cold stress response.

  2021cited by this paper
• Functional Analysis of Poplar SOMBRERO-type NAC Transcription Factors Yields a Strategy to Modify Woody Cell Wall Properties.

  2021cited by this paper
• ZmCCT regulates photoperiod-dependent flowering and response to stresses in maize

  2021cited by this paper
• Functional roles of the birch BpRAV1 transcription factor in salt and osmotic stress response.

  2021cited by this paper
• SlMYB102, an R2R3-type MYB gene, confers salt tolerance in transgenic tomato.

  2020cited by this paper
• The miR156/SPL module regulates apple salt stress tolerance by activating MdWRKY100 expression

  2020cited by this paper
• Osmoregulation and its actions during the drought stress in plants.

  2020cited by this paper
• Clock component OsPRR73 positively regulates rice salt tolerance by modulating OsHKT2;1‐mediated sodium homeostasis

  2020cited by this paper
• Overexpression of HcSCL13, a Halostachys caspica GRAS transcription factor, enhances plant growth and salt stress tolerance in transgenic Arabidopsis.

  2020cited by this paper
• Rice NAC transcription factor ONAC066 functions as a positive regulator of drought and oxidative stress response

  2019cited by this paper
• Overexpression of ThSAP30BP from Tamarix hispida improves salt tolerance.

  2019cited by this paper
• Stress-induced senescence and plant tolerance to abiotic stress

  2018cited by this paper
• Elucidating the molecular mechanisms mediating plant salt-stress responses.

  2018cited by this paper
• WRKY transcription factors in plant responses to stresses.

  2017cited by this paper
• ThDof1.4 and ThZFP1 constitute a transcriptional regulatory cascade involved in salt or osmotic stress in Tamarix hispida

  2017cited by this paper
• The transcriptome of NaCl-treated Limonium bicolor leaves reveals the genes controlling salt secretion of salt gland

  2016cited by this paper
• Wheat NAC transcription factor TaNAC29 is involved in response to salt stress.

  2015cited by this paper
• Research advances in major cereal crops for adaptation to abiotic stresses.

  2014cited by this paper
• Is proline accumulation per se correlated with stress tolerance or is proline homeostasis a more critical issue?

  2014cited by this paper
• Maize ABP9 enhances tolerance to multiple stresses in transgenic Arabidopsis by modulating ABA signaling and cellular levels of reactive oxygen species

  2011cited by this paper
• The salt- and drought-inducible poplar GRAS protein SCL7 confers salt and drought tolerance in Arabidopsis thaliana

  2010cited by this paper
• GRAS-domain transcription factors that regulate plant development

  2009cited by this paper
• Large-scale analysis of the GRAS gene family in Arabidopsis thaliana

  2008cited by this paper
• Salt stress increases the expression of p5cs gene and induces proline accumulation in cactus pear.

  2008cited by this paper
• Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression.

  2007cited by this paper
• Genome-Wide Analysis of Basic/Helix-Loop-Helix Transcription Factor Family in Rice and Arabidopsis1[W]

  2006cited by this paper
• Molecular mechanism of gibberellin signaling in plants.

  2004cited by this paper
• Evaluation of chlorophyll fluorescence and membrane injury in the leaves of barley cultivars under osmotic stress.

  2004cited by this paper
• The role of GRAS proteins in plant signal transduction and development

  2004cited by this paper
• Genome-Wide Analysis of the GRAS Gene Family in Rice and Arabidopsis

  2004cited by this paper
• Imaging of photo-oxidative stress responses in leaves.

  2002cited by this paper
• The GRAS gene family in Arabidopsis: sequence characterization and basic expression analysis of the SCARECROW-LIKE genes.

  1999cited by this paper
• Transcription factors: an overview.

  1997cited by this paper
• CHLOROPHYLL AND CAROTENOIDS: PIGMENTS OF PHOTOSYNTHETIC BIOMEMBRANES

  1987cited by this paper
• Genome-Wide Identification and Characterization of WRKY Transcription Factors in Betula platyphylla Suk. and Their Responses to Abiotic Stresses

  year unknowncited by this paper

• No citing papers are available for this paper.