Transcriptome analysis reveals the mechanism of anthocyanin accumulation induced by leaf breakage in foxtail millet

Anthocyanin is a class of water-soluble pigments with antioxidant activity which enhances the stress tolerance of plants. The destruction of vascular bundles may lead to the accumulation of anthocyanin; however, the response mechanism on this phenomenon in foxtail millet is not well understood. In this study, a foxtail millet variety Hong Miao Qi Tou Hong (HMQTH) which displayed increasing anthocyanin accumulation after leaf breakage was investigated. Through transcriptome analysis the gene expression pattern induced by leaf breakage in HMQTH was identified. Differentially expressed genes (DEGs) were mainly enriched in processes such as stimulus response, antioxidant function and flavonoid biosynthesis. Both the results of RNA-seq and qRT-PCR showed a total of 18 differential genes related to stress response and anthocyanin biosynthesis pathways were identified, including 7 genes involved in flavonoid and anthocyanin synthesis, 6 genes which were related to stress response and 5 genes regulating phytohormone synthesis and ABA signaling. This study uncovered the relationship between plant response to wounding and anthocyanin accumulation in foxtail millet, and provided genetic resources for the molecular breeding of crops with high anthocyanin content.

• Publication year

  2025

• Venue

  BMC Plant Biology

• Publication date

  2025-11-24

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1186/s12870-025-07806-xPMID 41286610PMCID 12763997
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2022influential reference
• Diverse roles of the CIPK gene family in transcription regulation and various biotic and abiotic stresses: A literature review and bibliometric study

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  2022cited by this paper
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• Abiotic stress-induced anthocyanins in plants: Their role in tolerance to abiotic stresses.

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  2020cited by this paper
• AtSUC1 introns act as strong enhancers of expression.

  2020cited by this paper
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  2020cited by this paper
• Maize SRO1e represses anthocyanin synthesis via regulating MBW complex in response to abiotic stress.

  2020cited by this paper
• MdWRKY40 promotes wounding-induced anthocyanin biosynthesis in association with MdMYB1 and undergoes MdBT2-mediated degradation.

  2019cited by this paper
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  2019cited by this paper
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  2019cited by this paper
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  2018cited by this paper
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  2018cited by this paper
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  2018cited by this paper
• The ethylene response factor MdERF1B regulates anthocyanin and proanthocyanidin biosynthesis in apple

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  2017cited by this paper
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  2017cited by this paper
• Transcriptomic Analysis of Leaf in Tree Peony Reveals Differentially Expressed Pigments Genes

  2017influential reference
• SENSITIVE TO FREEZING2 Aids in Resilience to Salt and Drought in Freezing-Sensitive Tomato1[OPEN]

  2016cited by this paper
• The Arabidopsis RCC1 Family Protein TCF1 Regulates Freezing Tolerance and Cold Acclimation through Modulating Lignin Biosynthesis

  2015cited by this paper
• StringTie enables improved reconstruction of a transcriptome from RNA-seq reads

  2015cited by this paper
• HISAT: a fast spliced aligner with low memory requirements

  2015cited by this paper
• Differential inductions of phenylalanine ammonia-lyase and chalcone synthase during wounding, salicylic acid treatment, and salinity stress in safflower, Carthamus tinctorius

  2014cited by this paper
• THF1 mutations lead to increased basal and wound-induced levels of oxylipins that stimulate anthocyanin biosynthesis via COI1 signaling in Arabidopsis.

  2014cited by this paper
• Anthocyanin biosynthesis for cold and freezing stress tolerance and desirable color in Brassica rapa

  2014cited by this paper
• Report elf Life of Tomatoes by Delaying Overripening and Reducing Susceptibility to Gray Mold

  2013cited by this paper
• SR1, a Calmodulin-Binding Transcription Factor, Modulates Plant Defense and Ethylene-Induced Senescence by Directly Regulating NDR1 and EIN31[W][OA]

  2012cited by this paper
• From landing lights to mimicry: the molecular regulation of flower colouration and mechanisms for pigmentation patterning.

  2012cited by this paper
• Freezing Tolerance in Plants Requires Lipid Remodeling at the Outer Chloroplast Membrane

  2010cited by this paper
• Anthocyanins: natural colorants with health-promoting properties.

  2010cited by this paper
• Light-induced vegetative anthocyanin pigmentation in Petunia

  2009cited by this paper
• Foxtail Millet: A Sequence-Driven Grass Model System[W]

  2009cited by this paper
• Tie-dyed2 Functions with Tie-dyed1 to Promote Carbohydrate Export from Maize Leaves1[C][W][OA]

  2008cited by this paper
• Biosynthesis of plant pigments: anthocyanins, betalains and carotenoids.

  2008cited by this paper
• Arabidopsis Sucrose Transporter AtSUC1 Is Important for Pollen Germination and Sucrose-Induced Anthocyanin Accumulation1[OA]

  2008cited by this paper
• A role for SENSITIVE TO FREEZING2 in protecting chloroplasts against freeze-induced damage in Arabidopsis.

  2008cited by this paper
• Tie-dyed1 and Sucrose export defective1 act independently to promote carbohydrate export from maize leaves

  2007cited by this paper
• Early Effects of Salinity on Water Transport in Arabidopsis Roots. Molecular and Cellular Features of Aquaporin Expression1

  2005cited by this paper
• Sucrose-Specific Induction of the Anthocyanin Biosynthetic Pathway in Arabidopsis[W]

  2005cited by this paper
• MYB-bHLH-WD40 protein complex and the evolution of cellular diversity.

  2005cited by this paper
• Flavonoids: a colorful model for the regulation and evolution of biochemical pathways.

  2005cited by this paper
• The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b

  2004cited by this paper
• Wounding stress increases the phenolic content and antioxidant capacity of purple-flesh potatoes (Solanum tuberosum L.).

  2003cited by this paper
• PIP1 plasma membrane aquaporins in tobacco: from cellular effects to function in plants.

  2002cited by this paper
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  2002cited by this paper
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  2001cited by this paper
• Antioxidant effect of red wine anthocyanins in normal and catalase-inactive human erythrocytes.

  2001cited by this paper
• Wound signalling in plants.

  2001cited by this paper
• Hydrogen peroxide is generated systemically in plant leaves by wounding and systemin via the octadecanoid pathway.

  1999cited by this paper
• Effect of concentrated red grape juice consumption on serum antioxidant capacity and low-density lipoprotein oxidation.

  1997cited by this paper
• Ethylene as a Signal Mediating the Wound Response of Tomato Plants

  1996cited by this paper
• Effect of red wine ingestion on the antioxidant capacity of serum.

  1995cited by this paper
• Genetics and Biochemistry of Anthocyanin Biosynthesis.

  1995cited by this paper
• Genetic and developmental control of anthocyanin biosynthesis.

  1991cited by this paper
• A Polypeptide from Tomato Leaves Induces Wound-Inducible Proteinase Inhibitor Proteins

  1991cited by this paper
• Malondialdehyde determination as index of lipid peroxidation.

  1990cited by this paper
• Abscisic acid is involved in the wound-induced expression of the proteinase inhibitor II gene in potato and tomato.

  1989cited by this paper
• Proteinase inhibitor-inducing factor activity in tomato leaves resides in oligosaccharides enzymically released from cell walls.

  1981cited by this paper

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