Acellular placental extract outcomes in post-traumatic osteoarthritis model

Objective Evaluate the effect of human placental extract (HPE) on healthy and osteoarthritic articular cartilage. Method Post-traumatic osteoarthritis (PTOA) rodent model was utilized to assess functional and histologic outcomes of HPE administration at 1, 2 and 3 months. Matrix metalloproteinase (MMP), glycosaminoglycan (GAG) content were assessed via human and porcine cartilage explants exposed to either HPE or saline cohorts. Results Improved gait function of PTOA-induced rodents treated with HPE in 5 measured parameters, persisting to the 3-month time point against the saline control. No significant difference in medial or lateral joint space was found on MicroCT evaluation after normalizing results to the contralateral uninjured limb. OARSI scores of the medial compartment of PTOA rodents at 3-months showed preservation of cartilage in the HPE-treated cohort with a score of 1.8 for HPE versus 9.17 for saline (p < 0.05). Porcine cartilage explants co-treated with HPE and IL-1 demonstrated marked reduction of MMP-1 (p = 0.001) and MMP-13 (p = 0.0023) expression compared to IL-1 alone. This effect was re-demonstrated in human cartilage with multiple MMP decreased. Reduced levels of GAG release occurred in HPE-treated cartilage explants in comparison to saline control (p = 0.004), with maintenance of structural architecture on histologic preparations. Conclusions HPE may be a promising treatment for OA by inhibiting the metabolic process of ECM breakdown and preserving the structural integrity of the knee joint in comparison to saline control.

• Publication year

  2025

• Venue

  Osteoarthritis and Cartilage Open

• Publication date

  2025-12-01

• Fields of study

  Medicine

• Identifiers
  DOI 10.1016/j.ocarto.2025.100729PMID 41540986PMCID 12799957
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Application of amniotic membrane in osteoarthritis management

  2024cited by this paper
• Effect of osteoarthritis and its surgical treatment on patients’ quality of life: a longitudinal study

  2023cited by this paper
• Pharmaceutical Treatment of Osteoarthritis.

  2022cited by this paper
• Osteoarthritis Treatment Guidelines from Six Professional Societies: Similarities and Differences.

  2022cited by this paper
• Peripheral Nerve Regeneration with Acellular Nerve Allografts Seeded with Amniotic Fluid-Derived Stem Cells

  2022cited by this paper
• Mesenchymal stem cells in osteoarthritis therapy: a review.

  2021cited by this paper
• A novel placental tissue biologic, PTP-001, inhibits inflammatory and catabolic responses in vitro and prevents pain and cartilage degeneration in a rat model of osteoarthritis.

  2021influential reference
• Recent Updates of Diagnosis, Pathophysiology, and Treatment on Osteoarthritis of the Knee

  2021cited by this paper
• Pathogenesis of Osteoarthritis: Risk Factors, Regulatory Pathways in Chondrocytes, and Experimental Models

  2020cited by this paper
• Early-stage knee OA induced by MIA and MMT compared in the murine model via histological and topographical approaches

  2020cited by this paper
• Cocktail of Hyaluronic Acid and Human Amniotic Mesenchymal Cells Effectively Repairs Cartilage Injuries in Sodium Iodoacetate-Induced Osteoarthritis Rats

  2020cited by this paper
• Can human placental extract help patients with osteoarthritis?

  2020cited by this paper
• Amniotic Suspension Allograft Modulates Inflammation in a Rat Pain Model of Osteoarthritis

  2019cited by this paper
• Evaluating the Effects of Platelet-Rich Plasma and Amniotic Viscous Fluid on Inflammatory Markers in a Human Coculture Model for Osteoarthritis.

  2019cited by this paper
• Inventor

  2019cited by this paper
• Human placental extract: a potential therapeutic in treating osteoarthritis

  2019cited by this paper
• A Randomized Controlled Single-Blind Study Demonstrating Superiority of Amniotic Suspension Allograft Injection Over Hyaluronic Acid and Saline Control for Modification of Knee Osteoarthritis Symptoms

  2019cited by this paper
• Human amniotic membrane intra-articular injection prevents cartilage damage in an osteoarthritis model

  2018cited by this paper
• Placenta and Placental Derivatives in Regenerative Therapies: Experimental Studies, History, and Prospects

  2018cited by this paper
• Placental therapy: An insight to their biological and therapeutic properties

  2017cited by this paper
• Current Treatment Options for Osteoarthritis.

  2017cited by this paper
• Efficacy of Particulate Amniotic Membrane and Umbilical Cord Tissues in Attenuating Cartilage Destruction in an Osteoarthritis Model

  2016cited by this paper
• Chondral and osteochondral operative treatment in early osteoarthritis

  2016cited by this paper
• On the predictive utility of animal models of osteoarthritis

  2015cited by this paper
• Call for standardized definitions of osteoarthritis and risk stratification for clinical trials and clinical use.

  2015cited by this paper
• Cryopreserved Amniotic Suspension for the Treatment of Knee Osteoarthritis

  2015cited by this paper
• Intra-articular injection of micronized dehydrated human amnion/chorion membrane attenuates osteoarthritis development

  2014cited by this paper
• Quality of life and self-reported disability in patients with knee osteoarthritis

  2013cited by this paper
• Full-Thickness Skin Wound Healing Using Human Placenta-Derived Extracellular Matrix Containing Bioactive Molecules

  2012cited by this paper
• Diagnosis of osteoarthritis: imaging.

  2012cited by this paper
• Current Surgical Treatment of Knee Osteoarthritis

  2011cited by this paper
• The OARSI histopathology initiative - recommendations for histological assessments of osteoarthritis in the rat.

  2010cited by this paper
• Protective effects of human placenta extract on cartilage degradation in experimental osteoarthritis.

  2010cited by this paper
• Treatment of osteoarthritis of the knee with microfracture and rehabilitation.

  2008cited by this paper
• Osteoarthritis cartilage histopathology: grading and staging.

  2006cited by this paper

• No citing papers are available for this paper.