Biotechnological Strategies for the Recovery of Lithium and Other Metals from a Secondary Source: The Role of Microorganisms and Metal-Binding Peptides

The growing demand for lithium, driven by its key role in rechargeable batteries and its use in electric vehicles, highlights the need for sustainable and environmentally friendly recovery strategies. Conventional methods, such as pyrometallurgy and hydrometallurgy, are effective but costly and harmful as they emit toxic compounds. Biohydrometallurgy has emerged as a promising alternative, as it uses microorganisms and their metabolites to solubilize metals under milder conditions. Biohydrometallurgy has emerged as a promising alternative, as it relies on microorganisms and their metabolites to solubilize metals under mild operating conditions. Nevertheless, challenges related to process efficiency and selectivity remain, particularly for lithium recovery. In this context, recent advances in metal-binding peptides have attracted increasing attention due to their inherent selectivity and the possibility of rational design and heterologous expression in well-established microbial hosts such as Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae. This review critically analyzes current biotechnological strategies and explores the integration of microbial bioleaching with peptide-based approaches as a complementary and environmentally friendly framework for the selective recovery of lithium and other metals from spent batteries and waste electrical and electronic equipment. Overall, this review provides an integrative conceptual framework that highlights the potential of combining microbial processes with metal-binding peptides to guide the development of more selective and sustainable biotechnological strategies for lithium recovery from secondary sources.

• Publication year

  2025

• Venue

  Recycling

• Publication date

  2025-12-24

• Fields of study

  Not labeled

• Identifiers
  DOI 10.3390/recycling11010004
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Fundamentals of bio-based technologies for selective metal recovery from bio-leachates and liquid waste streams

  2025influential reference
• A Novel Continuous Ultrasound-Assisted Leaching Process for Rare Earth Element Extraction: Environmental and Economic Assessment

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  2023influential reference
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• Bioaccumulation for heavy metal removal: a review

  2023influential reference
• Design of a Whole-Cell Biosensor Based on Bacillus subtilis Spores and the Green Fluorescent Protein To Monitor Arsenic

  2023cited by this paper
• Sustainable bioleaching of lithium-ion batteries for critical materials recovery

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• Saccharomyces cerevisiae cell surface display technology: Strategies for improvement and applications

  2022cited by this paper
• Construction of the lithium binding peptide displayed recombinant Escherichia coli for the specific lithium removal from various metal polluted wastewater

  2022influential reference
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  2021cited by this paper
• Metal extraction from spent lithium-ion batteries (LIBs) at high pulp density by environmentally friendly bioleaching process

  2021cited by this paper
• Lithium bioleaching: An emerging approach for the recovery of Li from spent lithium ion batteries.

  2021influential reference
• Bioprocessing of spent lithium ion batteries for critical metals recovery – A review

  2021cited by this paper
• Microbial recovery of critical metals from secondary sources.

  2021influential reference
• A review on the recycling of spent lithium-ion batteries (LIBs) by the bioleaching approach.

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• Bioleaching as an Eco-Friendly Approach for Metal Recovery from Spent NMC-Based Lithium-Ion Batteries at a High Pulp Density

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• A Review on Battery Market Trends, Second-Life Reuse, and Recycling

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• Comparison of three different bioleaching systems for Li recovery from lepidolite

  2020cited by this paper
• Biohydrometallurgical processes for the recovery of precious and base metals from waste electrical and electronic equipments: Current trends and perspectives

  2020influential reference
• Recovery of cobalt, lithium, and manganese from the cathode active materials of spent lithium-ion batteries in a bio-electro-hydrometallurgical process

  2019influential reference
• Engineering Biocatalytic and Biosorptive Materials for Environmental Applications.

  2019cited by this paper
• Bacillus subtilis Spore Surface Display Technology: A Review of Its Development and Applications.

  2019cited by this paper
• Environmentally friendly recovery of valuable metals from spent coin cells through two-step bioleaching using Acidithiobacillus thiooxidans.

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• Mechanism underlying the bioleaching process of LiCoO2 by sulfur-oxidizing and iron-oxidizing bacteria.

  2019cited by this paper
• Bacterial leaching as a green approach for typical metals recovery from end-of-life coin cells batteries

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• Accumulation and Release of Rare Earth Ions by Spores of Bacillus Species and the Location of These Ions in Spores

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• Membrane-based technologies for lithium recovery from water lithium resources: A review

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• Bioaccumulation of lead, chromium, and nickel by bacteria from three different genera isolated from industrial effluent

  2019cited by this paper
• Recovery of Metals from Waste Lithium Ion Battery Leachates Using Biogenic Hydrogen Sulfide

  2019cited by this paper
• Surface display of metal binding domain derived from PbrR on Escherichia coli specifically increases lead(II) adsorption

  2018cited by this paper
• Biological Leaching and Chemical Precipitation Methods for Recovery of Co and Li from Spent Lithium-Ion Batteries

  2018influential reference
• Application of a mixed culture of adapted acidophilic bacteria in two-step bioleaching of spent lithium-ion laptop batteries

  2018influential reference
• Heavy Metal Removal by Bioaccumulation Using Genetically Engineered Microorganisms

  2018cited by this paper
• Application of indirect non-contact bioleaching for extracting metals from waste lithium-ion batteries.

  2018cited by this paper
• Chromosomal expression of CadR on Pseudomonas aeruginosa for the removal of Cd(II) from aqueous solutions.

  2018cited by this paper
• Manganese and cobalt recovery by surface display of metal binding peptide on various loops of OmpC in Escherichia coli

  2017cited by this paper
• Lead (Pb) bioaccumulation; genera Bacillus isolate S1 and SS19 as a case study

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• Bioleaching Potential of Filamentous Fungi to Mobilize Lithium and Cobalt from Spent Rechargeable Li-Ion Batteries

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• Anchoring plant metallothioneins to the inner face of the plasma membrane of Saccharomyces cerevisiae cells leads to heavy metal accumulation

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• Cell Surface Display of MerR on Saccharomyces cerevisiae for Biosorption of Mercury

  2017cited by this paper
• Leaching of Metals from Spent Lithium-Ion Batteries

  2017cited by this paper
• Comparative assessment of metallurgical recovery of metals from electronic waste with special emphasis on bioleaching

  2017cited by this paper
• Bioleaching of valuable metals from spent lithium-ion mobile phone batteries using Aspergillus niger

  2016influential reference
• Bioleaching of valuable metals Li, Co, Ni and Mn from spent electric vehicle Li-ion batteries for the purpose of recovery

  2016cited by this paper
• Advance review on the exploitation of the prominent energy-storage element: Lithium. Part I: From mineral and brine resources

  2016cited by this paper
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• Enhanced tolerance and accumulation of heavy metal ions by engineered Escherichia coli expressing Pyrus calleryana phytochelatin synthase

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• Biotechnological recovery of heavy metals from secondary sources—An overview

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• Efficient binding of nickel ions to recombinant Bacillus subtilis spores.

  2010cited by this paper
• Investigation of heavy metals biosorption on Pseudomonas aeruginosa strain MCCB 102 isolated from the Persian Gulf.

  2010cited by this paper
• Sulphate-reducing Bacteria: Sulphate-reducing bacteria and their role in corrosion of ferrous materials

  2007cited by this paper
• Removal and recovery of lithium using various microorganisms.

  2005cited by this paper
• BIOSORPTION OF HEAVY METALS

  2003cited by this paper

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