Genetic analysis of a quantitative trait locus associated with resistance to the root-lesion nematode Pratylenchus neglectus in triticale

Message. A QTL from rye chromosome 5R confers resistance to root-lesion nematode in triticale. Root-lesion nematode (Pratylenchus neglectus, RLN) poses a significant threat to global wheat production. High levels of RLN resistance are rare in wheat. Triticale, an amphiploid generated by combining wheat and rye genomes that naturally carries rye-derived defense alleles, offers an untapped reservoir of nematode resistance. Here, we evaluated the response to RLN in 137 recombinant inbred lines (RILs) derived from a cross between two triticale cultivars: Siskiyou (susceptible) and Villax St. Jose (resistant). Genotyping-by-sequencing identified 1054 high-quality single-nucleotide polymorphism (SNP) markers, which, along with seven simple sequence repeat (SSR) markers, were assembled into 21 linkage groups covering the triticale genome. A single quantitative trait locus (QTL) on the rye-derived chromosome 5R was identified that explained approximately 20% of the phenotypic variance across experiments. A high-throughput Kompetitive allele-specific PCR (KASP) assay based on the most significant SNP marker was developed, providing a rapid genotyping platform for selecting the resistance allele and reducing reliance on labor-intensive phenotyping for P. neglectus resistance in triticale. This study reports the first mapped RLN-resistance QTL in triticale, laying the fundamental foundation for introgressing the 5R resistance allele into wheat via marker-assisted selection combined with chromosome engineering, thereby broadening the genetic basis for nematode resistance in cereal crops.

• Publication year

  2026

• Venue

  Theoretical and Applied Genetics

• Publication date

  2026-01-01

• Fields of study

  Biology, Agricultural and Food Sciences, Medicine, Environmental Science

• Identifiers
  DOI 10.1007/s00122-025-05112-6PMID 41489649PMCID 12769958
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Development of diagnostic markers for the disease susceptibility gene Tsn1 in wheat reveals novel resistance alleles and a new locus required for ToxA sensitivity

  2025cited by this paper
• Evolution, diversity, and function of the disease susceptibility gene Snn1 in wheat.

  2024cited by this paper
• Genetic mapping of QTLs for resistance to bacterial leaf streak in hexaploid wheat

  2024cited by this paper
• A Quantitative Trait Locus with a Major Effect on Root-Lesion Nematode Resistance in Barley

  2024cited by this paper
• Development of a Set of Wheat-Rye Derivative Lines from Hexaploid Triticale with Complex Chromosomal Rearrangements to Improve Disease Resistance, Agronomic and Quality Traits of Wheat

  2023cited by this paper
• Evaluation of Wheat Cultivars and Germplasm Lines for Resistance to Pratylenchus neglectus Populations Collected in North Dakota.

  2023influential reference
• A high-quality genome assembly highlights rye genomic characteristics and agronomically important genes

  2021cited by this paper
• Chromosome-scale genome assembly provides insights into rye biology, evolution and agronomic potential

  2021cited by this paper
• The Pros and Cons of Rye Chromatin Introgression into Wheat Genome

  2021cited by this paper
• Root-lesion Nematodes Affecting Dryland Cereals in the Semiarid Pacific Northwest USA.

  2021influential reference
• Fine mapping of root lesion nematode (Pratylenchus thornei) resistance loci on chromosomes 6D and 2B of wheat

  2019cited by this paper
• Triticale (x Triticosecale Wittmack) Breeding

  2019cited by this paper
• Triticale Improvement for Forage and Cover Crop Uses in the Southern Great Plains of the United States

  2018cited by this paper
• Transferring useful rye genes to wheat, using triticale as a bridge.

  2018cited by this paper
• Root-lesion nematodes in cereal fields: importance, distribution, identification, and management strategies

  2018cited by this paper
• Towards a whole‐genome sequence for rye (Secale cereale L.)

  2017cited by this paper
• Resistance to Multiple Soil-Borne Pathogens of the Pacific Northwest, USA Is Colocated in a Wheat Recombinant Inbred Line Population

  2017cited by this paper
• Constructing linkage maps in the genomics era with MapDisto 2.0

  2017influential reference
• Genetic mapping of a major gene in triticale conferring resistance to bacterial leaf streak

  2017influential reference
• Association analysis of resistance to cereal cyst nematodes (Heterodera avenae) and root lesion nematodes (Pratylenchus neglectus and P. thornei) in CIMMYT advanced spring wheat lines for semi-arid conditions

  2016influential reference
• Assessment and Management of Root Lesion Nematodes in Montana Wheat Production.

  2016cited by this paper
• Characterization of wheat-Secale africanum chromosome 5Ra derivatives carrying Secale specific genes for grain hardness

  2016cited by this paper
• Identification of Resistance to Pratylenchus neglectus and Pratylenchus thornei in Iranian Landrace Accessions of Wheat

  2016cited by this paper
• OF THE UNITED STATES

  2015cited by this paper
• PolyMarker: A fast polyploid primer design pipeline

  2015cited by this paper
• TASSEL-GBS: A High Capacity Genotyping by Sequencing Analysis Pipeline

  2014influential reference
• Chromosome composition of wheat-rye lines and the influence of rye chromosomes on disease resistance and agronomic traits

  2014cited by this paper
• Resistance and Tolerance of Landrace Wheat in Fields Infested with Pratylenchus neglectus and P. thornei.

  2014cited by this paper
• Development and characterization of wheat-rye lines combining T1RS·1BL translocation and 5R(5D) chromosome substitution or T1RS·1BL and T5AS·5RL translocations

  2014cited by this paper
• Association mapping for soilborne pathogen resistance in synthetic hexaploid wheat

  2013cited by this paper
• Genetic mapping and marker development for resistance of wheat against the root lesion nematode Pratylenchus neglectus

  2013cited by this paper
• Genetic mapping and marker development for resistance of wheat against the root lesion nematode Pratylenchus neglectus

  2013cited by this paper
• QTL mapping of multiple foliar disease and root-lesion nematode resistances in wheat

  2010cited by this paper
• Pratylenchus neglectus Reduces Yield of Winter Wheat in Dryland Cropping Systems.

  2009cited by this paper
• Nematodes which Challenge Global Wheat Production

  2009cited by this paper
• Detection and Discrimination of Pratylenchus neglectus and P. thornei in DNA Extracts from Soil.

  2008cited by this paper
• Pratylenchus (Nematoda: Pratylenchidae): Diagnosis, Biology, Pathogenicity and Management

  2008cited by this paper
• QGene 4.0, an extensible Java QTL-analysis platform

  2008influential reference
• Managing nematode pests in the southern and western regions of the Australian cereal industry: continuing progress in a challenging environment

  2008influential reference
• TASSEL: software for association mapping of complex traits in diverse samples

  2007influential reference
• Evaluating the Genetic Diversity of Triticale with Wheat and Rye SSR Markers

  2006cited by this paper
• Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status

  2004cited by this paper
• Publisher's Note

  2003cited by this paper
• Mapping of the root lesion nematode (Pratylenchus neglectus) resistance gene Rlnn1 in wheat

  2002cited by this paper
• Deletion Mapping of a Nematode Resistance Gene on Rye Chromosome 6R in Wheat

  2001cited by this paper
• Effect of Field Crops on Population Densities of Pratylenchus neglectus and P. thornei in Southeastern Australia; Part 1: P. neglectus.

  2000cited by this paper
• Measuring yield loss in cereals caused by root lesion nematodes (Pratylenchus neglectus and P. thornei) with and without nematicide

  1999cited by this paper
• Relationship between root lesion nematodes (Pratylenchus neglectus and P. thornei) and performance of wheat varieties

  1998cited by this paper
• Genetic variation in resistance to Pratylenchus neglectus in wheat and triticales

  1995influential reference
• Detection and Discrimination

  1991cited by this paper
• Resistance and tolerance

  1987cited by this paper
• Gnotobiotic Culture of Pratylenchus scribneri on Carrot Discs

  1986cited by this paper
• Registration of ‘Siskiyou’ Triticale

  1985cited by this paper
• A comparison of some quantitative methods of extracting small vermiform nematodes from soil

  1965cited by this paper
• The estimation of map distances from recombination values.

  1943cited by this paper

• No citing papers are available for this paper.