Maternal Determinants of Human Milk Leptin and Their Associations with Neonatal Growth Parameters

Introduction: Human breast milk (HBM) is a critical source of nourishment for newborns, containing bioactive compounds that influence infant growth and metabolic programming. Among these compounds, leptin—a hormone primarily produced by adipocytes but also synthesized in the mammary gland—has gathered attention for its potential role in regulating energy balance and body weight. This study investigates the influence of maternal factors on HBM leptin concentrations and explores their associations with neonatal growth parameters. Material and Methods: 262 HBM samples were collected from healthy lactating mothers through Spanish Biobanks during the first six months postpartum. Data on maternal characteristics (body mass index (BMI), age, physical activity, parity, and delivery type) and neonatal measurements (weight, length, and head circumference) were collected. Leptin concentrations in skimmed HBM were measured using the ELISA technique (R&D Systems™, Minneapolis, MN, USA). Statistical analyses were conducted using R version 4.3.1 and MATLAB R2023a, with significance set at p < 0.05. Results: Leptin levels were highest in and declined over time, reaching a stable level after the first month of lactation. Preterm deliveries exhibited significantly higher leptin concentrations than term deliveries (0.42 vs. 0.07 ng/mL). Higher leptin levels were also observed in younger and primiparous mothers. Maternal BMI was positively associated with leptin concentration, with mothers who had elevated BMI showing higher levels than those with optimal BMI (0.36 vs. 0.05 ng/mL). Maternal physical activity was not associated with leptin concentrations in univariate analyses; although greater self-reported physical activity appeared associated with lower leptin concentrations in regression models, this finding should be interpreted cautiously and should not be considered evidence of an independent or consistent effect. Neonatal growth parameters (weight, length, and head circumference) were negatively correlated with HBM leptin concentrations. Conclusions: Our findings indicate that leptin levels in breast milk reflect both maternal metabolic status and neonatal characteristics and may represent a compensatory mechanism in preterm infants. HBM leptin levels are modulated by maternal BMI, age, parity, and delivery type, and are associated with neonatal growth parameters.

• Publication year

  2026

• Venue

  Nutrients

• Publication date

  2026-01-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.3390/nu18020192PMID 41599805PMCID 12845080
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• Chemistry of Human Breast Milk-A Comprehensive Review of the Composition and Role of Milk Metabolites in Child Development.

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• Determinants of leptin in human breast milk: results of the Ulm SPATZ Health Study

  2018influential reference
• Adiponectin, leptin and insulin in breast milk: associations with maternal characteristics and infant body composition in the first year of life

  2018influential reference
• Exercise Increases Adiponectin and Reduces Leptin Levels in Prediabetic and Diabetic Individuals: Systematic Review and Meta-Analysis of Randomized Controlled Trials

  2018cited by this paper
• Leptin, An Adipokine With Central Importance in the Global Obesity Problem.

  2018cited by this paper
• The relationship between breast milk leptin and adiponectin with child body composition from 3 to 5 years: a follow‐up study

  2017cited by this paper
• Associations between human breast milk hormones and adipocytokines and infant growth and body composition in the first 6 months of life

  2017cited by this paper
• Review of Infant Feeding: Key Features of Breast Milk and Infant Formula

  2016cited by this paper
• A narrative review of the associations between six bioactive components in breast milk and infant adiposity

  2016cited by this paper
• Breastfeeding and Complementary Feeding.

  2016cited by this paper
• Breast milk leptin and adiponectin in relation to infant body composition up to 2 years

  2015cited by this paper
• Maternal Characteristics Associated with Milk Leptin Content in a Sample of Filipino Women and Associations with Infant Weight for Age

  2015cited by this paper
• Leptin applications in 2015: what have we learned about leptin and obesity?

  2015cited by this paper
• Human breast milk: A review on its composition and bioactivity.

  2015cited by this paper
• Lipid Quality in Infant Nutrition: Current Knowledge and Future Opportunities

  2015cited by this paper
• The Study of Breast Milk IGF-1, Leptin, Ghrelin and Adiponectin Levels as Possible Reasons of High Weight Gain in Breast-Fed Infants

  2014cited by this paper
• Comparative measurement of ghrelin, leptin, adiponectin, EGF and IGF-1 in breast milk of mothers with overweight/obese and normal-weight infants

  2014cited by this paper
• Effect of Maternal Body Mass Index on Hormones in Breast Milk: A Systematic Review

  2014cited by this paper
• Human milk composition: nutrients and bioactive factors.

  2013cited by this paper
• Relationship of insulin, glucose, leptin, IL‐6 and TNF‐α in human breast milk with infant growth and body composition

  2012cited by this paper
• Adiponectin, AFABP, and Leptin in Human Breast Milk During 12 Months of Lactation

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• Leptin in Maternal Serum and Breast Milk: Association With Infants' Body Weight Gain in a Longitudinal Study Over 6 Months of Lactation

  2011cited by this paper
• Leptin determination in colostrum and early human milk from mothers of preterm and term infants.

  2011cited by this paper
• Breast Milk Hormones and Their Protective Effect on Obesity

  2009cited by this paper
• The relationship between breast milk leptin and neonatal weight gain

  2009cited by this paper
• Adipokines in Human Milk and Risk of Overweight in Early Childhood: A Prospective Cohort Study

  2007cited by this paper
• The intake of physiological doses of leptin during lactation in rats prevents obesity in later life

  2007cited by this paper
• Leptin concentration in breast milk and its relationship to duration of lactation and hormonal status

  2006cited by this paper
• Adiponectin is present in human milk and is associated with maternal factors.

  2006cited by this paper
• A Physiological Role of Breast Milk Leptin in Body Weight Control in Developing Infants

  2006cited by this paper
• Longitudinal Investigation of the Relationship between Breast Milk Leptin Levels and Growth in Breast-fed Infants

  2005cited by this paper
• Time-course of leptin levels in term and preterm human milk.

  2004cited by this paper
• Breast milk leptin: its relationship to maternal and infant adiposity.

  2002influential reference
• Leptin levels in preterm human breast milk and infant formula.

  2001cited by this paper
• Breast milk: a truly functional food.

  2000cited by this paper
• Human leptin: from an adipocyte hormone to an endocrine mediator.

  2000cited by this paper
• The biology of leptin: a review.

  1998cited by this paper
• Leptin is present in human milk and is related to maternal plasma leptin concentration and adiposity.

  1997cited by this paper
• Nonadipose tissue production of leptin: Leptin as a novel placenta-derived hormone in humans

  1997cited by this paper
• Presence of leptin in colostrum and/or breast milk from lactating mothers: a potential role in the regulation of neonatal food intake.

  1997cited by this paper

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