Rutin Exhibits In-Vitro Anticoagulant Activity in Human Blood Samples by Prolonging Coagulation Pathway Times

Background Rutin is a plant-derived flavonoid with reported biological activities, but its effect on blood coagulation parameters has not been clearly characterized under in-vitro conditions. Objective This study aimed to evaluate the in-vitro anticoagulant activity of rutin in human blood by measuring changes in clotting time (CT), prothrombin time (PT), and activated partial thromboplastin time (aPTT) at increasing concentrations, compared with baseline control values. Materials and Methods Rutin (1-6 mg/mL) was added to human blood samples, and standard CT, PT, and aPTT assays were used to measure coagulation parameters. Heparin and Phosphate-buffered saline (PBS) were used as controls. Results In a concentration-dependent manner, rutin significantly increased activated partial thromboplastin time (aPTT), prothrombin time (PT), and clotting time (CT) compared with the PBS control, with the most pronounced effects observed at higher concentrations (4 and 6 mg/mL; P < 0.05 vs control). At 6 mg/mL, CT increased to 11.42 ± 1.11 minutes, PT to 21.33 ± 3.28 seconds, and aPTT to 57.20 ± 9.31 seconds. Conclusion Rutin exhibits significant in vitro anticoagulant activity, supporting its potential as a natural anticoagulant candidate.

• Publication year

  2026

• Venue

  Global Advances in Integrative Medicine and Health

• Publication date

  2026-01-19

• Fields of study

  Medicine

• Identifiers
  DOI 10.1177/27536130261419403PMID 41567822PMCID 12816505
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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