NMR-Based Quantification of Collagen Content in Protein Hydrolysates.

Certain functional food and dietary supplement ingredients contain bioactive peptides whose bioactivity is determined by their chain length, composition, and specific sequence of the amino acids. This study investigated collagenous peptides from the valorization of poultry side streams, which, for example, have inhibitory activities against therapeutic targets for the management of blood pressure and blood glucose. To overcome the specificity challenges of conventional amino acid analysis, the aim was to establish and validate a quantitative 1H NMR (qHNMR) method for the quantification of hydroxyproline as a marker of collagen content in poultry hydrolysates. Indeed, qHNMR provides a rapid structure-specific quantification, and it is highly informative, reproducible, and accurate. The effect of different acidic solvents was investigated using quantum mechanics (QM)-based 1H iterative functionalized spin analysis (HifSA). Comparison of conventional integration (int-) with QM-qHNMR revealed limitations of the former approach and highlighted the increased accuracy and specificity of QM-qHNMR.

• Publication year

  2026

• Venue

  Journal of Agricultural and Food Chemistry

• Publication date

  2026-02-08

• Fields of study

  Agricultural and Food Sciences, Medicine, Chemistry

• Identifiers
  DOI 10.1021/acs.jafc.5c13447PMID 41656696PMCID PMC12921863
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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