Live imaging of synapse development and measuring protein dynamics using two-color fluorescence recovery after photo-bleaching at Drosophila synapses

No abstract is available for this paper.

• Publication year

  2007

• Venue

  Nature Protocols

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/nprot.2007.472PMID 18079729
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The Ig cell adhesion molecule Basigin controls compartmentalization and vesicle release at Drosophila melanogaster synapses

  2007cited by this paper
• Thirty-One Flavors of Drosophila Rab Proteins

  2007cited by this paper
• Kinesin-1 and Dynein are the primary motors for fast transport of mitochondria in Drosophila motor axons.

  2006cited by this paper
• Shaggy/GSK-3beta kinase localizes to the centrosome and to specialized cytoskeletal structures in Drosophila.

  2006cited by this paper
• Bruchpilot Promotes Active Zone Assembly, Ca2+ Channel Clustering, and Vesicle Release

  2006cited by this paper
• Rapid Redistribution of Synaptic PSD-95 in the Neocortex In Vivo

  2006cited by this paper
• Bruchpilot, a protein with homology to ELKS/CAST, is required for structural integrity and function of synaptic active zones in Drosophila.

  2006cited by this paper
• A guide to choosing fluorescent proteins

  2005cited by this paper
• In vivo imaging of long-term changes in the Drosophila neuromuscular junction

  2005cited by this paper
• Glutamate receptor dynamics organizing synapse formation in vivo

  2005cited by this paper
• Four Different Subunits Are Essential for Expressing the Synaptic Glutamate Receptor at Neuromuscular Junctions of Drosophila

  2005cited by this paper
• Cell type-specific recruitment of Drosophila Lin-7 to distinct MAGUK-based protein complexes defines novel roles for Sdt and Dlg-S97

  2004cited by this paper
• Structural dynamics of synapses in living animals.

  2004cited by this paper
• Isoflurane depresses glutamate release by reducing neuronal excitability at the Drosophila neuromuscular junction

  2004cited by this paper
• Active Zone Localization of Presynaptic Calcium Channels Encoded by the cacophony Locus of Drosophila

  2004cited by this paper
• In Vivo Time-Lapse Imaging of Synaptic Takeover Associated with Naturally Occurring Synapse Elimination

  2003cited by this paper
• Role of Drosophila Rab5 during endosomal trafficking at the synapse and evoked neurotransmitter release

  2003cited by this paper
• Direct imaging of lateral movements of AMPA receptors inside synapses

  2003cited by this paper
• Experience-Dependent Strengthening of Drosophila Neuromuscular Junctions

  2003cited by this paper
• wishful thinking encodes a BMP type II receptor that regulates synaptic growth in Drosophila.

  2002cited by this paper
• Living synaptic vesicle marker: Synaptotagmin‐GFP

  2002cited by this paper
• New fluorescent protein reporters for use with the drosophila gal4 expression system and for vital detection of balancer chromosomes

  2002cited by this paper
• Breaking Abbe's diffraction resolution limit in fluorescence microscopy with stimulated emission depletion beams of various shapes.

  2001cited by this paper
• A conditional tissue-specific transgene expression system using inducible GAL4

  2001cited by this paper
• Fluorescence correlation spectroscopy reveals fast optical excitation-driven intramolecular dynamics of yellow fluorescent proteins.

  2000cited by this paper
• The fusome organizes the microtubule network during oocyte differentiation in Drosophila.

  2000cited by this paper
• Watching a synapse grow: noninvasive confocal imaging of synaptic growth in Drosophila.

  1999cited by this paper
• Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis.

  1999cited by this paper
• Genetic analysis of the mechanisms controlling target selection: target-derived Fasciclin II regulates the pattern of synapse formation.

  1997cited by this paper
• Regulation of synapse structure and function by the Drosophila tumor suppressor gene dlg.

  1996cited by this paper
• Green fluorescent protein as a vital marker and reporter of gene expression in Drosophila.

  1995cited by this paper
• Ectopic and increased expression of Fasciclin II alters motoneuron growth cone guidance.

  1994cited by this paper
• Distinct morphogenetic functions of similar small GTPases: Drosophila Drac1 is involved in axonal outgrowth and myoblast fusion.

  1994cited by this paper
• Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.

  1993cited by this paper
• Plasticity in the central nervous system: do synapses divide?

  1983cited by this paper
• Mobility measurement by analysis of fluorescence photobleaching recovery kinetics.

  1976cited by this paper

• Analysis of Protein and Lipid Dynamics Using Confocal Fluorescence Recovery After Photobleaching

  2026cites this paper
• Molecular glue for phycobilisome attachment to photosystem II in Synechococcus sp. PCC 7002

  2025influential citation
• What's past is prologue: FRAP keeps delivering 50 years later.

  2023cites this paper
• Influence of T-Bar on Calcium Concentration Impacting Release Probability

  2022cites this paper
• Dissection and Direct Imaging of Axonal Transport in Drosophila Segmental Nerves.

  2022cites this paper
• Microglia-dependent excessive synaptic pruning leads to cortical underconnectivity and behavioral abnormality following chronic social defeat stress in mice.

  2022cites this paper
• LarvaSPA, A Method for Mounting Drosophila Larva for Long-Term Time-Lapse Imaging.

  2020cites this paper
• Localized microinjection of intact Drosophila melanogaster larva to investigate the effect of serotonin on heart rate.

  2019cites this paper
• Chloroform and Desflurane Immobilization with Recovery of Viable Drosophila Larvae for Confocal Imaging.

  2019cites this paper
• Fly-on-a-Chip: Microfluidics for Drosophila melanogaster Studies.

  2019cites this paper
• Long-term in vivo imaging of Drosophila larvae

  2019cites this paper
• Motor circuit function is stabilized during postembryonic growth by anterograde trans-synaptic Jelly Belly - Anaplastic Lymphoma Kinase signaling

  2019cites this paper
• Presynaptic Biogenesis Requires Axonal Transport of Lysosome-Related Vesicles.

  2018cites this paper
• Rapid active zone remodeling consolidates presynaptic potentiation

  2018cites this paper
• Characterization of developmental and molecular factors underlying release heterogeneity at Drosophila synapses

  2018influential citation
• Glutamatergic Synthesis, Recycling, and Receptor Pharmacology at Drosophila and Crustacean Neuromuscular Junctions

  2018cites this paper
• Examining molecular determinants underlying heterogeneity of synaptic release probability using optical quantal imaging

  2017cites this paper
• Stable Positioning of Unc13 Restricts Synaptic Vesicle Fusion to Defined Release Sites to Promote Synchronous Neurotransmission.

  2017cites this paper
• Two kinesins drive anterograde neuropeptide transport

  2017cites this paper
• Scaling of cytoskeletal organization with cell size in Drosophila

  2017cites this paper
• The Crossroads of Synaptic Growth Signaling, Membrane Traffic and Neurological Disease: Insights from Drosophila

  2016cites this paper
• Characterization of axonal transport defects in Drosophila Huntingtin mutants

  2016cites this paper
• Active zone scaffolds differentially accumulate Unc13 isoforms to tune Ca2+ channel–vesicle coupling

  2016cites this paper
• Visualizing Neural Function

  2015cites this paper
• Lrrk regulates the dynamic profile of dendritic Golgi outposts through the golgin Lava lamp

  2015cites this paper
• Impaired retrograde transport by the Dynein/Dynactin complex contributes to Tau-induced toxicity.

  2015cites this paper
• A high affinity RIM-binding protein/Aplip1 interaction prevents the formation of ectopic axonal active zones

  2015cites this paper
• Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae

  2014cites this paper
• Spatial and Temporal Characteristics of Normal and Perturbed Vesicle Transport

  2014cites this paper
• In Vivo Immunostaining of Hemocyte Compartments in Drosophila for Live Imaging

  2014cites this paper
• Dynamic clonal analysis based on chronic in vivo imaging allows multiscale quantification of growth in the Drosophila wing disc

  2014influential citation
• Imaging through the pupal case of Drosophila melanogaster.

  2014cites this paper
• New Approaches for Studying Synaptic Development, Function, and Plasticity Using Drosophila as a Model System

  2013cites this paper
• The Kinesin-3, Unc-104 Regulates Dendrite Morphogenesis and Synaptic Development in Drosophila

  2013cites this paper
• Knockdown of Hsc70-5/mortalin Induces Loss of Synaptic Mitochondria in a Drosophila Parkinson’s Disease Model

  2013cites this paper
• New tools and new biology: Recent miniaturized systems for molecular and cellular biology

  2013cites this paper
• Pre- and Postsynaptic Assembly and Maturation

  2013cites this paper
• Near Infrared Microsensor for Continuous In-vivo Intraocular and Intracranial Pressure Monitoring.

  2013cites this paper
• The role of Huntingtin in fast axonal transport

  2012cites this paper
• The role of Kinesin-3 in regulating synaptic development in Drosophila

  2012cites this paper
• Cell Wall Amidase AmiC1 Is Required for Cellular Communication and Heterocyst Development in the Cyanobacterium Anabaena PCC 7120 but Not for Filament Integrity

  2012cites this paper
• ABSTRACTS FOR PLENARY LECTURES

  2012cites this paper
• Análisis molecular de la sinaptogénesis dependiente de PI3K en Drosophila

  2012cites this paper
• Three Routes to Suppression of the Neurodegenerative Phenotypes Caused by Kinesin Heavy Chain Mutations

  2012cites this paper
• In vivo imaging of the Drosophila larval neuromuscular junction.

  2012cites this paper
• Microfluidic Chips for In Vivo Imaging of Cellular Responses to Neural Injury in Drosophila Larvae

  2012cites this paper
• Huntingtin Aggregation Kinetics and Their Pathological Role in a Drosophila Huntington’s Disease Model

  2012cites this paper
• Spastic Paraplegia Mutation N256S in the Neuronal Microtubule Motor KIF5A Disrupts Axonal Transport in a Drosophila HSP Model

  2012cites this paper
• In vivo imaging of Drosophila larval neuromuscular junctions to study synapse assembly.

  2012cites this paper
• Presynaptic PI3K activity triggers the formation of glutamate receptors at neuromuscular terminals of Drosophila

  2012cites this paper
• Building an imaging chamber for in vivo imaging of Drosophila larvae.

  2012cites this paper
• Axonal transport disruption in peripheral nerve disease

  2012cites this paper
• In-Vivo Imaging of the Drosophila Wing Imaginal Disc over Time: Novel Insights on Growth and Boundary Formation

  2012cites this paper
• Störungen des axonalen Transports bei der hereditären spastischen Spinalparalyse im Drosophila -Modell

  2011cites this paper
• Label-free visualization of ultrastructural features of artificial synapses via cryo-EM.

  2011cites this paper
• The morphogene AmiC2 is pivotal for multicellular development in the cyanobacterium Nostoc punctiforme

  2011cites this paper
• Kinesin-1 tail autoregulation and microtubule-binding regions function in saltatory transport but not ooplasmic streaming

  2011cites this paper
• Visualizing Nervous System Function

  2010cites this paper
• In vivo Imaging of Intact Drosophila Larvae at Sub-cellular Resolution

  2010cites this paper
• PP2AandGSK-3ActAntagonisticallytoRegulateActive ZoneDevelopment

  2009cites this paper
• Maturation of active zone assembly by Drosophila Bruchpilot

  2009cites this paper
• PP2A and GSK-3β Act Antagonistically to Regulate Active Zone Development

  2009cites this paper
• Positional cloning by fast-track SNP-mapping in Drosophila melanogaster

  2008cites this paper
• In Vivo Imaging of Drosophila Larval Neuromuscular Junctions to Study Synapse Assembly 409 Imaging of the Fly Nmj to Study Synapse Assembly

  year unknowncites this paper