Plackett-Burman Design and Response Surface Optimization of Medium Trace Nutrients for Glycolipopeptide Biosurfactant Production

Background: A glycolipopeptide biosurfactant produced by Pseudomonas aeruginosa strain IKW1 reduced the surface tension of fermentation broth from 71.31 to 24.62 dynes/cm at a critical micelle concentration of 20.80 mg/L. The compound proved suitable for applications in emulsion stabilization in food, as well as in cosmetic and pharmaceutical formulations. Methods: In the present study, Plackett-Burman design (PBD) and response surface method (RSM) were employed to screen and optimize concentrations of trace nutrients in the fermentation medium, to increase surfactant yield. Results: The PBD selected 5 significant trace nutrients out of the 12 screened. The RSM, on the other hand, resulted in the production of 84.44 g glycolipopeptide/L in the optimized medium containing 1.25 mg/L nickel, 0.125 mg/L zinc, 0.075 mg/L iron, 0.0104 mg/L boron, and 0.025 mg/L copper. Conclusion: Significant second-order quadratic models for biomass (P<0.05; adjusted R2=94.29%) and biosurfactant (R2=99.44%) responses suggest excellent goodness-of-fit of the models. However, their respective non-significant lack-of-fit (Biomass: F=1.28; P=0.418; Biosurfactant: F=1.20; P=0.446) test results indicate their adequacy to explain data variations in the experimental region. The glycolipopeptide is recommended for the formulation of inexpensive pharmaceutical products that require surface-active compounds.

• Publication year

  2017

• Venue

  Iranian Biomedical Journal

• Publication date

  2017-04-23

• Fields of study

  Medicine, Chemistry, Engineering, Environmental Science

• Identifiers
  DOI 10.18869/acadpub.ibj.21.4.249PMID 28433004PMCID 5459940
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Optimization of rhamnolipid biosurfactant production by mangrove sediment bacterium Pseudomonas aeruginosa KVD-HR42 using response surface methodology

  2016cited by this paper
• A Pseudomonas aeruginosa Strain Ikw1 Produces an Unusual Polymeric Surface-Active Compound in Waste Frying Oil-Minimal Medium

  2016influential reference
• Biosurfactants in agriculture

  2013cited by this paper
• Optimization Methods: From Theory to Design Scientific and Technological Aspects in Mechanics

  2012cited by this paper
• ISOLATION, IDENTIFICATION & PHYLOGENETIC ANALYSIS OF PHTHALIC ACID DEGRADING BACTERIA

  2012cited by this paper
• Dietary boron: progress in establishing essential roles in human physiology.

  2012cited by this paper
• Enhanced biosurfactant production through cloning of three genes and role of esterase in biosurfactant release

  2011cited by this paper
• Environmental Applications of Biosurfactants: Recent Advances

  2011cited by this paper
• Biosurfactant Production by Pseudomonas aeruginosa from Renewable Resources

  2011cited by this paper
• Microbial biosurfactants production, applications and future potential

  2010cited by this paper
• Evaluation of different carbon and nitrogen sources in production of biosurfactant by Pseudomonas fluorescens

  2008cited by this paper
• Biosurfactant production by Corynebacterium kutscheri from waste motor lubricant oil and peanut oil cake

  2007cited by this paper
• Statistical optimization of medium components for the production of biosurfactant by Bacillus licheniformis K51.

  2007cited by this paper
• Response surface optimization of the medium components for the production of biosurfactants by probiotic bacteria

  2006cited by this paper
• Distribution of Biosurfactant-Producing Bacteria in Undisturbed and Contaminated Arid Southwestern Soils

  2003cited by this paper
• Utilization of response surface methodology to optimize the culture media for the production of rhamnolipids by Pseudomonas aeruginosa AT10

  2002cited by this paper
• Natural roles of biosurfactants.

  2001cited by this paper
• Production of a Peptidoglycolipid Bioemulsifier by Pseudomonas aeruginosa Grown on Hydrocarbon

  2001cited by this paper
• Microbial heavy-metal resistance

  1999cited by this paper
• Cytochrome c oxidase: structure and spectroscopy.

  1998cited by this paper
• Microbial production of surfactants and their commercial potential

  1997cited by this paper
• A new lipopeptide biosurfactant produced by Arthrobacter sp. strain MIS38

  1993cited by this paper
• The product of the hypB gene, which is required for nickel incorporation into hydrogenases, is a novel guanine nucleotide-binding protein

  1993cited by this paper
• Annual review of biophysics and biomolecular structure

  1992cited by this paper

• Characterization of chromogenic bacteria using agro-waste extracts as substrate for pigments production

  2026cites this paper
• Application of statistical design of experiment to identify key factors in cross coupling reactions

  2025cites this paper
• Technological advancement in sustainable production of biosurfactant using agro-industrial wastes: A review of enhanced bioprocessing and computational optimization techniques

  2025cites this paper
• Microbial Pigment Production for Sustainable Production: a review

  2025cites this paper
• Insight into the eminent biotechnological applications of xylanolytic enzymes for sustainable bioprocessing

  2025cites this paper
• Optimization and kinetics study of α-amylase production by the newly isolated thermophilic yeast strain Geotrichum candidum PO27 using olive pomace waste under solid-state fermentation

  2025cites this paper
• Screening of factors influencing riboflavin producing recombinant Ashbya Gossypii using Plackett-Burman fractional factorial design experiment

  2024cites this paper
• Medium Optimization for Biobutanol Production From Palm Kernel Cake (PKC) Hydrolysate By Clostridium saccharoperbutylacetonicum N1-4

  2024cites this paper
• Optimized Rhamnolipid Production by a Pseudomonas marginalis C9 Strain Isolated from a Biopurification System to Enhance Pesticide Solubilization

  2024cites this paper
• Enhancing pigment production by a chromogenic bacterium (Exiguobacterium aurantiacum) using tomato waste extract: A statistical approach

  2024cites this paper
• Optimization of culture condition for Spodoptera frugiperda by design of experiment approach and evaluation of its effect on the expression of hemagglutinin protein of influenza virus

  2024cites this paper
• A study on end mill tool geometry parameters for end milling of 316L: finite element analysis and response surface methodology optimization based on resultant cutting force

  2024cites this paper
• Enhancement of vitamin B6 production driven by omics analysis combined with fermentation optimization

  2024cites this paper
• Recent advances on biodiesel production from waste cooking oil (WCO): A review of reactors, catalysts, and optimization techniques impacting the production

  2023cites this paper
• Characterization of an isolated lactase enzyme produced by Bacillus licheniformis ALSZ2 as a potential pharmaceutical supplement for lactose intolerance

  2023cites this paper
• Arctic Sea Ice Microalga Chlamydomonas latifrons KNF0041: Identification and Statistical Optimization of Medium for Enhanced Biomass and Omega-3/Omega-6

  2023cites this paper
• Cleavage of macromolecule (protein/polysaccharide)-phenolic bond in soybean cell wall through Lactobacillus casei and Lactobacillus helviticus mixed culture solid-state fermentation for chlorogenic acid extraction

  2023cites this paper
• A Comprehensive Review on RSM-Coupled Optimization Techniques and Its Applications

  2023cites this paper
• Application of the Plackett-Burman design for screening and optimisation of factors affecting the aqueous extract for total anthocyanin content of broken riceberry rice

  2023cites this paper
• APPLICATION OF PLACKETT-BURMAN AND BOX-BEHNKEN DESIGNS FOR SCREENING AND OPTIMIZATION OF ROTIGOTINE HCL AND RASAGILINE MESYLATE TRANSFERSOMES: A STATISTICAL APPROACH

  2023cites this paper
• Statistical optimization strategies on waste substrates for solving high-cost challenges in biosurfactants production: a review

  2023influential citation
• Effective use of screening experiments: Some practical experiences

  2023cites this paper
• A Comparative Study of optimized conditions of QuEChERS to determine the pesticide multiresidues in Lycium barbarum using Response Surface Methodology and Genetic Algorithm-Artificial Neural Network

  2023cites this paper
• A novel strain of Stenotrophomonas acidaminiphila produces thermostable alkaline peptidase on agro-industrial wastes: process optimization, kinetic modeling and scale-up

  2022cites this paper
• An insight - A statistical investigation of consolidated bioprocessing of Allium ascalonicum leaves to ethanol using Hangateiclostridium thermocellum KSMK1203 and synthetic consortium

  2022cites this paper
• Biphasic Liquid-Liquid Extraction of Biosurfactant from Lactobacillus delbrueckii

  2022cites this paper
• Statistical optimization of phytol and polyunsaturated fatty acid production in the Antarctic microalga Micractinium variabile KSF0031

  2022cites this paper
• Bioprocess development as a sustainable platform for eco-friendly alkaline phosphatase production: an approach towards crab shells waste management

  2022cites this paper
• Optimization, purification, and characterization of xylanase production by a newly isolated Trichoderma harzianum strain by a two-step statistical experimental design strategy

  2022cites this paper
• Efficient Ultrasonic-assisted Aqueous Enzymatic Method for Pecan Nut Kernel Oil Extraction with Quality Analysis.

  2022cites this paper
• Optimization of Lipase Production by a Newly Isolate of Lactobacillus Fermentum

  2022cites this paper
• Pigment Productions by Spirulina platensis as a Renewable Resource

  2022cites this paper
• Predictive capability evaluation and optimization of sustainable biodiesel production from oleaginous biomass grown on pulp and paper industrial wastewater

  2021cites this paper
• Bioprocess Optimization of Nutritional Parameters for Enhanced Anti-leukemic L-Asparaginase Production by Aspergillus candidus UCCM 00117: A Sequential Statistical Approach

  2021cites this paper
• FITTING PLACKETT-BURMAN DESIGN ON CHILD DISEASE EXPERIMENT

  2021cites this paper
• MARINE CYANOBACTERIUM OSCILLATORIA ACUTISSIMA 海洋蓝藻尖锐湿疣生产胞外嗜碱淀粉酶

  2021cites this paper
• Kinetic modeling and quasi-economic analysis of fermentative glycolipopeptide biosurfactant production in a medium co-optimized by statistical and neural network approaches

  2021cites this paper
• Potential of Aspergilus Niger MT809753 for Bio-treatment Paper Industry Wastewater After Screening Effective Factors on Production of Cellulase Enzyme by Plackett-burman Design

  2021cites this paper
• Statistical sequential optimization of process parameters for inulinase production by Kluyveromyces marxianus ATCC 36907 in solid-state fermentation using beer residue

  2021cites this paper
• Chemo-metric engineering designs for deciphering the biodegradation of polycyclic aromatic hydrocarbons.

  2021cites this paper
• A novel strain of Stenotrophomonas acidaminiphila produces super-active alkaline protease during cassava effluent fermentation: process optimization, kinetic modeling and scale-up

  2021cites this paper
• Improvement of tacrolimus production in Streptomyces tsukubaensis by mutagenesis and optimization of fermentation medium using Plackett–Burman design combined with response surface methodology

  2021cites this paper
• Development of a New Bioprocess for Clean Diosgenin Production through Submerged Fermentation of an Endophytic Fungus

  2021cites this paper
• Multivariate optimization of liquid chromatographic conditions for determination of dapagliflozin and saxagliptin, application to an in vitro dissolution and stability studies

  2021cites this paper
• Effects of Sand/Fly Ash and the Water/Solid Ratio on the Mechanical Properties of Engineered Geopolymer Composite and Mix Design Optimization

  2020cites this paper
• Co-culture of Trichoderma atroviride SG3403 and Bacillus subtilis 22 improves the production of antifungal secondary metabolites

  2020cites this paper
• “Screening and Optimization of Agro-industrial wastes for glycoprotein biosurfactant production from Sphingobacterium thalpophilum DP9”

  2020cites this paper
• Production, purification and application of Cutinase in enzymatic scouring of cotton fabric isolated from Acinetobacter baumannii AU10

  2020cites this paper
• Modeling methods used in bioenergy productionprocesses: A review

  2020cites this paper
• Statistical and Artificial Neural Network Approaches to Modeling and Optimization of Fermentation Conditions for Production of a Surface/Bioactive Glyco-lipo-peptide

  2020cites this paper
• Biosurfactants: Production and application prospects in the food industry

  2020cites this paper
• Development of a fast method for the determination of pesticide multiresidues in tomatoes using QuEChERS and GC–MS/MS

  2020cites this paper
• MULTIVARIATE STATISTICS OF FERTILITY PARAMETER FLUXES IN CEMENT-DUST-POLLUTED SOILS IN MFAMOSING, NIGERIA: IMPACT ON AGRICULTURE

  2020cites this paper
• Production of the Anti-Leukemic Therapeutic Enzyme, L-Asparaginase, by A Brackish Sediment Strain of Aspergillus candidus

  2019cites this paper
• Comparative Analysis of Determination Methods of Glyphosate Degradation by Trichoderma asperellum Strain JK-28: A Multivariate Statistical Approach

  2019cites this paper
• Efficient aqueous enzymatic-ultrasonication extraction of oil from Sapindus mukorossi seed kernels

  2019cites this paper
• Detection of Five Mycotoxins in Different Food Matrices in the Malaysian Market by Using Validated Liquid Chromatography Electrospray Ionization Triple Quadrupole Mass Spectrometry

  2019cites this paper
• A serum-free medium suitable for maintaining cell morphology and liver-specific function in induced human hepatocytes

  2019cites this paper
• Application of Agaricus bisporus industrial wastewater to produce the biomass of Pichia burtonii.

  2019cites this paper
• Application of statistical design to evaluate critical process parameters and optimize formulation technique of polymeric nanoparticles

  2019cites this paper
• Statistical Distribution of Halotolerant/Halophilic Biosurfactant-Producing Yeasts in Qua Iboe Estuarine Sediments

  2019cites this paper
• Process optimization and adsorption modeling of Pb(II) on nickel ferrite-reduced graphene oxide nano-composite

  2018cites this paper
• Optimization of Cultural Conditions for Bacillus megaterium Cultured in Agaricus bisporus Industrial Wastewater

  2018cites this paper
• Plackett-Burman design for screening of process components and their effects on production of lactase by newly isolated Bacillus sp. VUVD101 strain from Dairy effluent

  2018cites this paper
• Simultaneous elimination of Malachite Green, Rhodamine B and Cresol Red from aqueous sample with Sistan sand, optimized by Taguchi L16 and Plackett–Burman experiment design methods

  2018cites this paper
• Research Medium Optimization for Biobutanol Production From Palm Kernel Cake (PKC) Hydrolysate By Clostridium saccharoperbutylacetonicum N1-4

  year unknowncites this paper
• Casting a Spotlight on Factorial Design: Exploring the Power of DoE for Experiment Screening and Optimization: A Review

  year unknowncites this paper
• Process optimization and adsorption modeling of Pb ( II ) on nickel ferrite-reduced graphene oxide nanocomposite

  year unknowncites this paper