Modulated Alignment Dual-Axis (MAD) Confocal Microscopy for Deep Optical Sectioining in Tissues

We present a strategy to preserve contrast over a deeper range of imaging depths in the context of confocal microscopy using low-power (0.5 mW) diode laser illumination. While the dual-axis confocal microscope architecture's intersecting illumination and collection beams significantly improves the spatial-filtering and optical-sectioning performance of confocal microscopy, we propose that modulating the spatial alignment of the dual-axis beams at a frequency f will generate a focal volume signal modulated at 2f, which further provides nearly an order-of-magnitude improvement in optical-sectioning contrast. Lock-in detection is used to remove the unmodulated background light, thereby enhancing our ability to image deeply within highly scattering tissues.

• Publication year

  2014

• Venue

  International Symposium on Optomechatronic Technologies

• Publication date

  2014-05-19

• Fields of study

  Medicine, Materials Science, Engineering

• Identifiers
  DOI 10.1117/12.2057734PMID 24940534PMCID PMC4052905
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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