Ontogeny and Phagocytic Function of Baboon Lung Dendritic Cells

Dendritic cells (DCs) are the most potent antigen‐presenting cells, but the ontogeny and functions of lung DCs are not known during prenatal period. Here, we isolated lung DC population from fetal (125–175 days of gestation age) and adult baboons. The cells were stained with fluorochrome‐conjugated‐HLA‐DP, DQ, DR, CD1a, CD11c, CD14, CD40, CD80, CD86, CD209, CMKLR1, ILT7‐specific antibodies, and staining was analyzed by flow cytometry. The phagocytic function was investigated by incubating the cells with fluorescent‐labeled Escherichia coli bioparticles and analyzed by flow cytometry and fluorescence microscopy. The fetal baboon lung DCs expressed low levels of HLA‐DP, DQ, DR, CD11c and CD86 as compared to adult baboon lung DCs and showed distinct DC morphology. The fetal lung DCs were also less capable of phagocytosing E. coli as compared to the adult lung DCs (P<0.05). In conclusion, the fetal lung DCs are not only phenotypically immature, but also less efficient in phagocytosing E. coli.

• Publication year

  2009

• Venue

  Immunology and Cell Biology

• Publication date

  2009-01-16

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/icb.2009.2PMID 19223853PMCID 2705687
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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