Ultrastructures and Classification of Circulating Hemocytes in 9 Botryllid Ascidians (Chordata: Ascidiacea)

Abstract Ultrastructures of circulating hemocytes were studied in 9 botryllid ascidians. The hemocytes are classified into five types: hemoblasts, phagocytes, granulocytes, morula cells, and pigment cells. These five types are always found in the 9 species. They should represent the major hemocyte types of the circulating cells in the blood. Hemoblasts are small hemocytes having a high nucleus/cytoplasm ratio. There are few granular or vacuolar inclusions in the cytoplasm. Phagocytes have phagocytic activity and their shape is variable depending on the amount of engulfed materials. In granulocytes, shape and size of granules are different among the species. Morula cells are characterized by several vacuoles filled with electron dense materials. In pigment cells, the bulk of the cytoplasm is occupied by one or a few vacuoles containing pigment granules. We also described some other hemocyte types found in particular species. Furthermore, we encountered free oocytes circulating in the blood in two species, Botryllus primigenus and Botrylloides lentus.

• Publication year

  2003

• Venue

  Zoological Science

• Publication date

  2003-05-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.2108/zsj.20.647PMID 12777836
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Colonial Allorecognition, Hemolytic Rejection, and Viviparity in Botryllid Ascidians

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  2001cited by this paper
• Morpho-functional characterization of haemocytes of the compound ascidian Botrylloides leachi (Tunicata, Ascidiacea)

  2001cited by this paper
• Immunolocation of phenoloxidase in vacuoles of the compound ascidian Botryllus schlosseri morula cells

  2000cited by this paper
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  2000cited by this paper
• Pigment Cells Representing Polychromatic Colony Color in Botrylloides simodensis (Ascidiacea, Urochordata): Cell Morphology and Pigment Substances

  1998cited by this paper
• Phenoloxidase and cytotoxicity in the compound ascidian Botryllus schlosseri.

  1998cited by this paper
• EVOLUTION OF ALLORECOGNITION IN BOTRYLLID ASCIDIANS INFERRED FROM A MOLECULAR PHYLOGENY

  1998cited by this paper
• Subcuticular Rejection: an Advanced Mode of the Allogeneic Rejection in the Compound Ascidians Botrylloides simodensis and B. fuscus.

  1997cited by this paper
• New data on phagocytes and phagocytosis in the compound ascidian Botryllus schlosseri (Tunicata, Ascidiacea)

  1996cited by this paper
• Characterization of Coelomocytes of the Ascidian Halocynthia roretzi Based on Phase-contrast, Time-lapse Video and Scanning Electron Microscopic Observations

  1995cited by this paper
• Ultrastructural Study of Oogenesis in the Compound Ascidian Botryllus schlosseri(Tunicata)

  1994cited by this paper
• Histoenzymatic staining and characterization of the colonial ascidian Botryllus schlösseri hemocytes

  1993influential reference
• Egg envelope cytodifferentiation in the colonial ascidian Botryllus schlosseri (Tunicata)

  1993cited by this paper
• Tunic Cell Morphology and Classification in Botryllid Ascidians(Morphology)

  1991cited by this paper
• Separation and labeling of specific subpopulations of Botryllus blood cells.

  1984cited by this paper
• Blood Cell Ultrastructure of the Ascidian Botryllus schlosseri L. II. Pigment Cells

  1983influential reference
• MORPHOLOGY AND GENETICS OF REJECTION REACTIONS BETWEEN OOZOOIDS FROM THE TUNICATE BOTRYLLUS SCHLOSSERI.

  1983cited by this paper
• Blood Cell Ultrastructure of the Ascidian Botryllus schlosseri.

  1978influential reference
• Studies on the formation of germ cells in a compound ascidian Botryllus, primigenus oka

  1976influential reference
• The Biology of Ascidians

  1971cited by this paper
• VASCULAR BUDDING, A NEW TYPE OF BUDDING IN BOTRYLLUS ,

  1957influential reference

• Single-cell transcriptomic profiling of the whole colony of Botrylloides diegensis: insights into tissue specialization and blastogenesis

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  2024cites this paper
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  2024cites this paper
• “Keep on rolling”: circulating cells in a botryllid ascidian torpor

  2023cites this paper
• Transcriptome landscapes that signify Botrylloides leachi (Ascidiacea) torpor states.

  2022cites this paper
• Evolution of immune defence responses as incremental layers among Metazoa

  2021cites this paper
• An Efficient Low-Cost Laboratory Workflow for the Study of Blood Cells and RNA Extractions in Marine Invertebrates

  2021cites this paper
• Evolution of colonial life history in styelids tunicates involves changes in complexity patterns

  2020cites this paper
• Evolutionary perspective on the hematopoietic system through a colonial chordate: allogeneic immunity and hematopoiesis.

  2020cites this paper
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  2019cites this paper
• Novel budding mode in Polyandrocarpa zorritensis: a model for comparative studies on asexual development and whole body regeneration

  2019cites this paper
• Features of a novel protein, rusticalin, from the ascidian Styela rustica reveal ancestral horizontal gene transfer event

  2019cites this paper
• Putative stem cells in the hemolymph and in the intestinal submucosa of the solitary ascidian Styela plicata

  2019cites this paper
• Homologues of p48 Protein from Morula Cells of Ascidian Styela rustica in Other Species of Stolidobranchia

  2019cites this paper
• The Phylogenetic Profi le of Mast Cells

  2018cites this paper
• Structural and Mass Spectrometric Imaging Analyses of Adhered Tunic and Adhesive Projections of Solitary Ascidians

  2018cites this paper
• Circulatory stem cells of Styela plicata (Lesueur, 1823) (Tunicata: Stelidae): an evolutionary approach

  2018cites this paper
• The haemocytes of the colonial aplousobranch ascidian Diplosoma listerianum: Structural, cytochemical and functional analyses.

  2017cites this paper
• Insights into the unique torpor of Botrylloides leachi, a colonial urochordate.

  2017cites this paper
• Flow cytometric characterization of hemocytes of the solitary ascidian, Halocynthia roretzi

  2017cites this paper
• Vascular budding in Symplegma brakenhielmi and the evolution of coloniality in styelid ascidians.

  2017cites this paper
• Histological and haematological analysis of the ascidian Botrylloides leachii (Savigny, 1816) during whole-body regeneration

  2017influential citation
• Cytotoxic Cells of Compound Ascidians

  2016cites this paper
• Dynamics of hemocyte subsets from ascidian Halocynthia aurantium in response to tissue damage: a comparative analysis of flow cytometry vs confocal microscopy data

  2016cites this paper
• Application of flow cytometry for the analysis of circulating hemocyte populations in the ascidian Halocynthia aurantium (Pallas, 1787)

  2015cites this paper
• The phylogenetic profile of mast cells.

  2015cites this paper
• Regulation of nitric-oxide production in hemocytes of the ascidian Phallusia nigra.

  2014cites this paper
• Classification and Ultrastructure of Hemocytes in the Tunicate Halocynthia roretzi (Ascidiacea: Pyuridae)

  2012influential citation
• Studies on Japanese Botryllid Ascidians. V. A New Species of the Genus Botrylloides Very Similar to Botrylloides simodensis in Morphology

  2011cites this paper
• Spatial competition induces the mobilization of morula cells in the colonial ascidian Didemnum perlucidum (Tunicata: Didemnidae)

  2011cites this paper
• Flow cytometry analysis of softness syndrome effects on hemocytes of the tunicate Halocynthia roretzi

  2010cites this paper
• The mast cell: an evolutionary perspective

  2010cites this paper
• Piwi‐expressing hemoblasts serve as germline stem cells during postembryonic germ cell specification in colonial ascidian, Botryllus primigenus

  2010cites this paper
• Ascidian tunic cells: morphology and functional diversity of free cells outside the epidermis

  2009cites this paper
• Nitric oxide production by hemocytes of the ascidian Styela plicata

  2009cites this paper
• Algal Symbionts in the Larval Tunic Lamellae of the Colonial Ascidian Lissoclinum timorense (Ascidiacea, Didemnidae)

  2008cites this paper
• Hemocyte differentiation in the juveniles of the ascidian Halocynthia roretzi

  2008cites this paper
• Immunobiology of compound ascidians, with particular reference to Botryllus schlosseri: state of art

  2008cites this paper
• Immune responses of mussel hemocyte subpopulations are differentially regulated by enzymes of the PI 3-K, PKC, and ERK kinase families.

  2008cites this paper
• The Hemolymph of the Ascidian Styela plicata (Chordata-Tunicata) Contains Heparin inside Basophil-like Cells and a Unique Sulfated Galactoglucan in the Plasma*

  2007cites this paper
• RGD-containing molecules induce macropinocytosis in ascidian hyaline amoebocytes.

  2006cites this paper
• fester, A candidate allorecognition receptor from a primitive chordate.

  2006cites this paper
• Divergent roles of the DEAD‐box protein BS‐PL10, the urochordate homologue of human DDX3 and DDX3Y proteins, in colony astogeny and ontogeny

  2006cites this paper
• In Vitro Culture of Mesenchymal Lineage Cells Established from the Colonial Tunicate Botryllus primigenus

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• Cytochemical properties of Botryllus schlosseri haemocytes: indications for morpho-functional characterisation.

  2005cites this paper
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  2005cites this paper
• Multidisciplinary Studies of the Genus "Cystodytes" (Ascidiacea): From Molecules to Species

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• Immunoglobulin superfamily receptors in protochordates: before RAG time

  2004cites this paper