The Downstream Regulatory Element of the proU Operon of Salmonella typhimurium Inhibits Open Complex Formation by RNA Polymerase at a Distance*

The intracellular concentration of K+-glutamate, chromatin-associated proteins, and a downstream regulatory element (DRE) overlapping with the coding sequence, have been implicated in the regulation of theproU operon of Salmonella typhimurium. The basal expression of the proU operon is low, but it is rapidly induced when the bacteria are grown in media of high osmolarity (e.g. 0.3 m NaCl). It has previously been suggested that increased intracellular concentrations of K+-glutamate activate the proU promoter in response to increased extracellular osmolarity. We show here that the activation of the proU promoter by K+-glutamatein vitro is nonspecific, and the in vivoregulation cannot simply be mimicked in vitro. In vivospecificity requires both the chromatin-associated protein H-NS and the DRE; they are both needed to maintain repression of proU expression at low osmolarity. How H-NS and the DRE repress the proUpromoter in vivo has so far been unclear. We show that,in vivo, the DRE acts at a distance to inhibit open complex formation at the proU promoter.

• Publication year

  2000

• Venue

  Journal of Biological Chemistry

• Publication date

  2000-04-21

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/JBC.275.16.12123PMID 10766847
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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