Specific Binding of Bacillus thuringiensis Cry2A Insecticidal Proteins to a Common Site in the Midgut of Helicoverpa Species

No abstract is available for this paper.

• Publication year

  2008

• Venue

  Applied and Environmental Microbiology

• Publication date

  2008-10-17

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1128/AEM.01373-08PMID 18931285PMCID PMC2607167
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2007influential reference
• Role of Receptors in Bacillus thuringiensis Crystal Toxin Activity

  2007cited by this paper
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• comprehensive molecular insect science

  2004cited by this paper
• Interaction of Bacillus thuringiensis Toxins with Larval Midgut Binding Sites of Helicoverpa armigera (Lepidoptera: Noctuidae)

  2004cited by this paper
• Bacillus thuringiensis delta-endotoxin binding to brush border membrane vesicles of rice stem borers.

  2004cited by this paper
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  2004cited by this paper
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  2003cited by this paper
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  2003cited by this paper
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  2000cited by this paper
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  2000cited by this paper
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  1999cited by this paper
• Bacillus thuringiensis and Its Pesticidal Crystal Proteins

  1998cited by this paper
• Determination of Binding of Bacillus thuringiensis (delta)-Endotoxin Receptors to Rice Stem Borer Midguts

  1997influential reference
• Proteolytic Processing ofBacillus thuringiensisCryIIIA Toxin and Specific Binding to Brush-Border Membrane Vesicles ofLeptinotarsa decemlineata(Colorado Potato Beetle)

  1996cited by this paper
• Selection and Genetic Analysis of a Heliothis virescens (Lepidoptera: Noctuidae) Strain with High Levels of Resistance to Bacillus thuringiensis Toxins

  1995cited by this paper
• Biochemistry and genetics of insect resistance to Bacillus thuringiensis.

  1995cited by this paper
• A specific binding protein from Tenebrio molitor for the insecticidal toxin of Bacillus thuringiensis subsp. tenebrionis.

  1994cited by this paper
• Mode of action of CryIIA: a Bacillus thuringiensis delta-endotoxin

  1994influential reference
• Mode of action of Bacillus thuringiensis toxin CryIIIA: An analysis of toxicity in Leptinotarsa decemlineata (Say) and Diabrotica undecimpunctata howardi Barber

  1992cited by this paper
• Broad-spectrum resistance to Bacillus thuringiensis toxins in Heliothis virescens.

  1992cited by this paper
• Receptors on the brush border membrane of the insect midgut as determinants of the specificity of Bacillus thuringiensis delta-endotoxins

  1990cited by this paper
• Insecticidal crystal proteins of Bacillus thuringiensis.

  1989cited by this paper
• Amino acid sequence and entomocidal activity of the P2 crystal protein. An insect toxin from Bacillus thuringiensis var. kurstaki.

  1988cited by this paper
• Preparation and partial characterization of amino acid transporting brush border membrane vesicles from the larval midgut of the cabbage butterfly (Pieris brassicae)

  1987cited by this paper
• Commitment of bacterial spores to germinate. A measure of the trigger reaction.

  1981cited by this paper
• Ligand: a versatile computerized approach for characterization of ligand-binding systems.

  1980cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper

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• Integrated genomic and proteomic analysis of local Bacillus thuringiensis isolates for targeted insect pest control and functional insight

  2025cites this paper
• Resistance of Lepidopteran Pests to Bacillus thuringiensis Toxins: Evidence of Field and Laboratory Evolved Resistance and Cross-Resistance, Mode of Resistance Inheritance, Fitness Costs, Mechanisms Involved and Management Options

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