Expression, purification and preliminary biochemical and structural characterization of the leucine rich repeat namesake domain of leucine rich repeat kinase 2.

No abstract is available for this paper.

• Publication year

  2012

• Venue

  Biochimica et Biophysica Acta

• Publication date

  2012-03-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.bbapap.2011.12.009PMID 22251894
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• O 1-7-4-2 Abnormal autophagy / lysosome function may play some role in Parkinson ’ s disease

  2018cited by this paper
• Insight into the mode of action of the LRRK2 Y1699C pathogenic mutant

  2011cited by this paper
• On the Road to Leucine-Rich Repeat Kinase 2 Signalling: Evidence from Cellular and in vivo Studies

  2011cited by this paper
• A leucine-rich repeat assembly approach for homology modeling of the human TLR5-10 and mouse TLR11-13 ectodomains

  2011cited by this paper
• LRRK2 signaling pathways: the key to unlocking neurodegeneration?

  2011cited by this paper
• What genetics tells us about the causes and mechanisms of Parkinson's disease.

  2011cited by this paper
• Human leucine-rich repeat proteins: a genome-wide bioinformatic categorization and functional analysis in innate immunity

  2010cited by this paper
• Identification and Characterization of a Leucine-Rich Repeat Kinase 2 (LRRK2) Consensus Phosphorylation Motif

  2010cited by this paper
• Genetic Etiology of Parkinson Disease Associated with Mutations in the SNCA, PARK2, PINK1, PARK7, and LRRK2 Genes: A Mutation Update

  2010cited by this paper
• Phosphopeptide analysis reveals two discrete clusters of phosphorylation in the N-terminus and the Roc domain of the Parkinson-disease associated protein kinase LRRK2.

  2010cited by this paper
• Mechanisms in dominant parkinsonism: The toxic triangle of LRRK2, α‐synuclein, and tau

  2010cited by this paper
• Genome-Wide Association Study reveals genetic risk underlying Parkinson’s disease

  2009cited by this paper
• A general protocol for the crystallization of membrane proteins for X-ray structural investigation

  2009cited by this paper
• A generic method for assignment of reliability scores applied to solvent accessibility predictions

  2009cited by this paper
• The Parkinson's disease kinase LRRK2 autophosphorylates its GTPase domain at multiple sites.

  2009cited by this paper
• Quantitative analysis and prediction of curvature in leucine‐rich repeat proteins

  2009cited by this paper
• The R1441C mutation alters the folding properties of the ROC domain of LRRK2.

  2009cited by this paper
• Relative contribution of simple mutations vs. copy number variations in five Parkinson disease genes in the Belgian population

  2009cited by this paper
• LRRK2 in Parkinson’s disease: genetic and clinical studies from patients

  2009cited by this paper
• LRRK2 mutations and risk variants in Japanese patients with Parkinson's disease

  2009cited by this paper
• Homo‐ and heterodimerization of ROCO kinases: LRRK2 kinase inhibition by the LRRK2 ROCO fragment

  2009cited by this paper
• Modeling of protein refolding from inclusion bodies.

  2009cited by this paper
• Dependence of Leucine-rich Repeat Kinase 2 (LRRK2) Kinase Activity on Dimerization*

  2009cited by this paper
• Clinical and pathological characteristics of patients with Leucine‐rich repeat kinase‐2 mutations

  2009cited by this paper
• LRRK2 regulates synaptic vesicle endocytosis.

  2008cited by this paper
• LRRML: a conformational database and an XML description of leucine-rich repeats (LRRs)

  2008cited by this paper
• MetaMQAP: A meta-server for the quality assessment of protein models

  2008cited by this paper
• The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation*

  2008cited by this paper
• Structure of the Roc–COR domain tandem of C. tepidum, a prokaryotic homologue of the human LRRK2 Parkinson kinase

  2008cited by this paper
• Corticobasal syndrome and primary progressive aphasia as manifestations of LRRK2 gene mutations

  2008cited by this paper
• Structure of the ROC domain from the Parkinson's disease-associated leucine-rich repeat kinase 2 reveals a dimeric GTPase

  2008cited by this paper
• The leucine-rich repeat structure

  2008cited by this paper
• The Parkinson's disease-associated protein, leucine-rich repeat kinase 2 (LRRK2), is an authentic GTPase that stimulates kinase activity.

  2007cited by this paper
• GTP binding is essential to the protein kinase activity of LRRK2, a causative gene product for familial Parkinson's disease.

  2007cited by this paper
• Soybean disease resistance protein RHG1-LRR domain expressed, purified and refolded from Escherichia coli inclusion bodies: preparation for a functional analysis.

  2007cited by this paper
• Production of active eukaryotic proteins through bacterial expression systems: a review of the existing biotechnology strategies

  2007cited by this paper
• LRRK2 mutation analysis in Parkinson disease families with evidence of linkage to PARK8

  2007cited by this paper
• The Parkinson disease causing LRRK2 mutation I2020T is associated with increased kinase activity.

  2006cited by this paper
• LRRK2 in Parkinson's disease: protein domains and functional insights.

  2006cited by this paper
• In vitro characterization of the cysteine-rich capping domains in a plant leucine rich repeat protein.

  2006cited by this paper
• The Structure of the Lingo-1 Ectodomain, a Module Implicated in Central Nervous System Repair Inhibition*

  2006cited by this paper
• Leucine-rich repeat kinase 2: a new player with a familiar theme for Parkinson's disease pathogenesis.

  2005cited by this paper
• Lrrk2 pathogenic substitutions in Parkinson's disease

  2005cited by this paper
• Comprehensive evaluation of common genetic variation within LRRK2 reveals evidence for association with sporadic Parkinson's disease.

  2005cited by this paper
• Type and frequency of mutations in the LRRK2 gene in familial and sporadic Parkinson's disease*.

  2005cited by this paper
• Structural analysis of leucine-rich-repeat variants in proteins associated with human diseases

  2005cited by this paper
• PIRLs: a novel class of plant intracellular leucine-rich repeat proteins.

  2005cited by this paper
• Mutations in LRRK2 cause autosomal-dominant parkinsonism with pleomorphic pathology.

  2004cited by this paper
• The identification of pats1, a novel gene locus required for cytokinesis in Dictyostelium discoideum.

  2003cited by this paper
• Roc, a Ras/GTPase domain in complex proteins.

  2003cited by this paper
• Designing repeat proteins: modular leucine-rich repeat protein libraries based on the mammalian ribonuclease inhibitor family.

  2003cited by this paper
• RanGAP mediates GTP hydrolysis without an arginine finger

  2002cited by this paper
• The leucine-rich repeat as a protein recognition motif.

  2001cited by this paper
• Structure-function analysis of Yersinia pestis YopM's interaction with alpha-thrombin to rule on its significance in systemic plague and to model YopM's mechanism of binding host proteins.

  2001cited by this paper
• Identification and predicted structure of a leucine-rich repeat motif shared by Leishmania major proteophosphoglycan and Parasite Surface Antigen 2.

  2000cited by this paper
• Bacterial expression and refolding of different fragments of human CD14

  2000cited by this paper
• The Acid-labile Subunit of the Serum Insulin-like Growth Factor-binding Protein Complexes

  1999cited by this paper
• SUR-8, a conserved Ras-binding protein with leucine-rich repeats, positively regulates Ras-mediated signaling in C. elegans.

  1998cited by this paper
• 14-3-3 Proteins Are Required for Maintenance of Raf-1 Phosphorylation and Kinase Activity

  1998cited by this paper
• Structural diversity of leucine-rich repeat proteins.

  1998cited by this paper
• Identification of the Binding Partners for Flightless I, A Novel Protein Bridging the Leucine-rich Repeat and the Gelsolin Superfamilies*

  1998cited by this paper
• The Acidic C-terminal Domain of rna1p Is Required for the Binding of Ran·GTP and for RanGAP Activity*

  1997cited by this paper
• In vitro folding of inclusion body proteins

  1996cited by this paper
• Structural and functional diversity in the leucine-rich repeat family of proteins.

  1996cited by this paper
• Purification and characterization of decorin core protein expressed in Escherichia coli as a maltose-binding protein fusion.

  1996cited by this paper
• Proteins with leucine-rich repeats.

  1995cited by this paper
• Modeling of the three-dimensional structure of proteins with the typical leucine-rich repeats.

  1995cited by this paper
• The leucine-rich repeat: a versatile binding motif.

  1994cited by this paper
• RanGAP1 induces GTPase activity of nuclear Ras-related Ran.

  1994cited by this paper
• Crystal structure of porcine ribonuclease inhibitor, a protein with leucine-rich repeats

  1993cited by this paper
• Isolation of rsp-1, a novel cDNA capable of suppressing v-Ras transformation

  1992cited by this paper
• Quantitative analysis of protein far UV circular dichroism spectra by neural networks.

  1992cited by this paper
• Protein Aggregation in vitro and in vivo: A Quantitative Model of the Kinetic Competition between Folding and Aggregation

  1991cited by this paper
• The use of sarkosyl in generating soluble protein after bacterial expression.

  1991cited by this paper
• Cloning and characterization of CAP, the S. cerevisiae gene encoding the 70 kd adenylyl cyclase-associated protein.

  1990cited by this paper
• Sodium dodecyl sulfate-protein polypeptide complexes in 8 M urea with special reference to sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

  1979cited by this paper

• Strengths, Weaknesses, Opportunities, and Potential Threat Analysis of Targeting Leucine-Rich Repeat Kinase 2 for Parkinson's Disease Treatment: Status Quo and Quo Vadis.

  2025cites this paper
• LRRK2 Structure-Based Activation Mechanism and Pathogenesis

  2023cites this paper
• Structural Advancement in Shoc2‐MAPK Signaling Pathways in the Treatment of Cancer and Other Diseases

  2022cites this paper
• BORCS6 is involved in the enlargement of lung lamellar bodies in Lrrk2 knockout mice

  2021cites this paper
• LRRK2 Targeting Strategies as Potential Treatment of Parkinson’s Disease

  2021cites this paper
• Direct evidence for a new mode of plant defense against insects via a novel polygalacturonase-inhibiting protein expression strategy

  2020cites this paper
• Clinically Precedented Protein Kinases: Rationale for Their Use in Neurodegenerative Disease

  2020cites this paper
• Physiological and pathological functions of LRRK2: implications from substrate proteins

  2018cites this paper
• Cryo-EM analysis of homodimeric full-length LRRK2 and LRRK1 protein complexes

  2017cites this paper
• Structural insights obtained for homodimeric full-length LRRK2/LRRK1 protein complexes and liposomal preparation as a tool to study membrane proteins under buffer gradients by Cryo-EM

  2017influential citation
• LRRK2 interacts with ATM and regulates Mdm2–p53 cell proliferation axis in response to genotoxic stress

  2017cites this paper
• Molecular dynamics simulations to understand LRKK2 mutations in Parkinson

  2016cites this paper
• First model of dimeric LRRK2: the challenge of unrevealing the structure of a multidomain Parkinson's-associated protein.

  2016cites this paper
• Control of Autophagy in Parkinson’s Disease

  2015cites this paper
• Phosphatases of α-synuclein, LRRK2, and tau: important players in the phosphorylation-dependent pathology of Parkinsonism

  2014cites this paper
• Prediction of the Repeat Domain Structures and Impact of Parkinsonism‐Associated Variations on Structure and Function of all Functional Domains of Leucine‐Rich Repeat Kinase 2 (LRRK2)

  2014cites this paper
• Differential protein–protein interactions of LRRK1 and LRRK2 indicate roles in distinct cellular signaling pathways

  2014influential citation
• Leucine-Rich Repeat Kinase 2 (LRRK2) Inhibitors

  2014cites this paper
• Lack of Correlation between the Kinase Activity of LRRK2 Harboring Kinase-Modifying Mutations and Its Phosphorylation at Ser910, 935, and Ser955

  2014cites this paper
• Structural and functional in silico analysis of LRRK2 missense substitutions

  2014cites this paper
• Structural biology of the LRRK2 GTPase and kinase domains: implications for regulation

  2014cites this paper
• An investigation into the molecular aetiology of Parkinson's disease in South African patients

  2013cites this paper
• Down-regulation of LRRK2 in control and DAT transfected HEK cells increases manganese-induced oxidative stress and cell toxicity.

  2013cites this paper
• Drosophila Suppressor/Enhancer Screen to Identify Novel LRRK2 Interactors

  2013cites this paper
• MODELING LRRK2-ASSOCIATED PARKINSON’S DISEASE IN C. ELEGANS

  2012cites this paper
• LRRK2: function and dysfunction Analysis of LRRK2 accessory repeat domains: prediction of repeat length, number, and sites of Parkinson's disease mutations

  2012cites this paper
• The IkappaB Kinase Family Phosphorylates the Parkinson’s Disease Kinase LRRK2 at Ser935 and Ser910 during Toll-Like Receptor Signaling

  2012cites this paper
• Analysis of LRRK2 accessory repeat domains: prediction of repeat length, number and sites of Parkinson's disease mutations.

  2012cites this paper