Design and use of C-terminal minigene vectors for studying role of heterotrimeric G proteins.

No abstract is available for this paper.

• Publication year

  2002

• Venue

  Methods in Enzymology

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/S0076-6879(02)44705-2PMID 11771412
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Calcium phosphate transfection of mammalian cultured cells.

  2000cited by this paper
• A Dominant-Negative Strategy for Studying Roles of G Proteins in Vivo *

  1999cited by this paper
• Number and Stoichiometry of Subunits in the Native Atrial G-protein-gated K+ Channel, IKACh *

  1998cited by this paper
• The Many Faces of G Protein Signaling*

  1998cited by this paper
• Antagonists of the Receptor-G Protein Interface Block Gi-coupled Signal Transduction*

  1998cited by this paper
• Gβγ Binding to GIRK4 Subunit Is Critical for G Protein-gated K+ Channel Activation*

  1998cited by this paper
• Targeted inactivation of alphai2 or alphai3 disrupts activation of the cardiac muscarinic K+ channel, IK+Ach, in intact cells.

  1997cited by this paper
• Targeted inactivation of αi2 or αi3 disrupts activation of the cardiac muscarinic K+ channel, IK+Ach, in intact cells

  1997cited by this paper
• How receptors talk to trimeric G proteins.

  1997cited by this paper
• Receptor-regulated ion channels.

  1997cited by this paper
• Potent Peptide Analogues of a G Protein Receptor-binding Region Obtained with a Combinatorial Library (*)

  1996cited by this paper
• Mechanisms of K+ Channel Regulation

  1996cited by this paper
• Heterotrimeric G proteins.

  1996cited by this paper
• Identification of a receptor/G-protein contact site critical for signaling specificity and G-protein activation.

  1995cited by this paper
• Functional analysis of a dominant negative mutant of G alpha i2.

  1995cited by this paper
• The G-protein-gated atrial K+ channel IKAch is a heteromultimer of two inwardly rectifying K+-channel proteins

  1995cited by this paper
• An Effector Site That Stimulates G-protein GTPase in Photoreceptors (*)

  1995cited by this paper
• Down‐regulation of A1 adenosine receptors coupled to muscarinic K+ current in cultured guinea‐pig atrial myocytes.

  1995cited by this paper
• Gβγ Binds Directly to the G Protein-gated K+ Channel, IKACh(*)

  1995cited by this paper
• Activation of muscarinic K+ current in guinea‐pig atrial myocytes by sphingosine‐1‐phosphate.

  1995cited by this paper
• G-protein regulation of ion channels.

  1995cited by this paper
• Desensitization and Internalization of the m2 Muscarinic Acetylcholine Receptor Are Directed by Independent Mechanisms *

  1995cited by this paper
• Structural and functional relationships of heterotrimeric G‐proteins

  1995cited by this paper
• Functional Analysis of a Dominant Negative Mutant of Gαi2(*)

  1995cited by this paper
• Synthetic peptides as probes for G protein function. Carboxyl-terminal G alpha s peptides mimic Gs and evoke high affinity agonist binding to beta-adrenergic receptors.

  1994cited by this paper
• Expression of a mutant Gi2 alpha subunit inhibits ATP and thrombin stimulation of cytoplasmic phospholipase A2-mediated arachidonic acid release independent of Ca2+ and mitogen-activated protein kinase regulation.

  1994cited by this paper
• Specific peptide probes for G-protein interaction with effectors.

  1994cited by this paper
• Visual identification of individual transfected cells for electrophysiology using antibody-coated beads.

  1994cited by this paper
• Pertussis toxin blocks M2 muscarinic receptor-mediated effects on contraction and cyclic AMP in the guinea pig ileum, but not M3-mediated contractions and phosphoinositide hydrolysis.

  1994cited by this paper
• Transfected muscarinic acetylcholine receptors selectively couple to Gi-type G proteins and Gq/11.

  1994cited by this paper
• Identification of residues critical for Ras(17N) growth-inhibitory phenotype and for Ras interaction with guanine nucleotide exchange factors

  1994cited by this paper
• Transfected m2 muscarinic acetylcholine receptors couple to G alpha i2 and G alpha i3 in Chinese hamster ovary cells. Activation and desensitization of the phospholipase C signaling pathway.

  1993cited by this paper
• Random mutagenesis of G protein alpha subunit G(o)alpha. Mutations altering nucleotide binding.

  1993cited by this paper
• Kinetic and structural analysis of the Mg(2+)-binding site of the guanine nucleotide-binding protein p21H-ras.

  1993cited by this paper
• The G226A mutant of Gs alpha highlights the requirement for dissociation of G protein subunits.

  1992cited by this paper
• The m2 muscarinic acetylcholine receptors are coupled to multiple signaling pathways via pertussis toxin-sensitive guanine nucleotide regulatory proteins.

  1991cited by this paper
• Activating and inactivating mutations of the alpha subunit of Gi2 protein have opposite effects on proliferation of NIH 3T3 cells.

  1991cited by this paper
• A dominant negative G alpha s mutant is rescued by secondary mutation of the alpha chain amino terminus.

  1991cited by this paper
• Site of G protein binding to rhodopsin mapped with synthetic peptides from the alpha subunit.

  1988cited by this paper
• The effect of GTP and Mg2+ on the GTPase activity and the fluorescent properties of Go.

  1987cited by this paper
• Effects of Mg2+ and the beta gamma-subunit complex on the interactions of guanine nucleotides with G proteins.

  1987cited by this paper

• Structure and function of adenosine receptor heteromers

  2021cites this paper
• Amphetamines signal through intracellular TAAR1 receptors coupled to Gα13 and GαS in discrete subcellular domains

  2019cites this paper
• The gep Proto-Oncogene G&agr;13 Mediates Lysophosphatidic Acid-Mediated Migration of Pancreatic Cancer Cells

  2013cites this paper
• Characterization of signaling pathways coupled to melatonin receptors in gastrointestinal smooth muscle.

  2013cites this paper
• Lysophosphatidic acid increases proximal tubule cell secretion of profibrotic cytokines PDGF-B and CTGF through LPA2- and Gαq-mediated Rho and αvβ6 integrin-dependent activation of TGF-β.

  2012cites this paper
• Lysophosphatidic Acid Increases Proximal Tubule Cell Secretion of Profibrotic Cytokines PDGF-B and CTGF through LPA2- and G (cid:1) q-Mediated Rho and (cid:1) v (cid:2) 6 Integrin-Dependent Activation of TGF- (cid:2)

  2012cites this paper
• Targeted nonviral gene-based inhibition of Gα(i/o)-mediated vagal signaling in the posterior left atrium decreases vagal-induced atrial fibrillation.

  2011cites this paper
• Traditional GPCR Pharmacology and Beyond

  2010cites this paper
• Signal Transduction: Pathways, Mechanisms and Diseases

  2010cites this paper
• Estrogen receptor-beta prevents cardiac fibrosis.

  2010cites this paper
• Impaired M3 Muscarinic Acetylcholine Receptor Signal Transduction Through Blockade of Binding of Multiple Proteins to its Third Intracellular Loop

  2010cites this paper
• Identification of PAR1-G protein signalling pathways involved in thrombin-induced CCL2/MCP-1 production

  2008cites this paper
• G(q)-dependent signalling by the lysophosphatidic acid receptor LPA(3) in gastric smooth muscle: reciprocal regulation of MYPT1 phosphorylation by Rho kinase and cAMP-independent PKA.

  2008cites this paper
• Thrombin induces fibroblast CCL2/JE production and release via coupling of PAR1 to Galphaq and cooperation between ERK1/2 and Rho kinase signaling pathways.

  2008cites this paper
• Coupling of M3 Acetylcholine Receptor to Gq16 Activates a Natriuretic Peptide Receptor Guanylyl Cyclase

  2007cites this paper
• Characterization of the molecular mechanisms of the coupling between intracellular loops of prostacyclin receptor with the C-terminal domain of the Galphas protein in human coronary artery smooth muscle cells.

  2006cites this paper
• Signal Transduction Mediated by Heptahelical Receptors and Heterotrimeric G Proteins

  2006cites this paper
• Gα13 Mediates a Signal That Is Essential for Proliferation and Survival of Thymocyte Progenitors

  2004cites this paper
• G (cid:1) 13 Mediates a Signal That Is Essential for Proliferation and Survival of Thymocyte Progenitors

  2004cites this paper
• Distinctive G protein-dependent signaling in smooth muscle by sphingosine 1-phosphate receptors S1P1 and S1P2.

  2004cites this paper
• Selective inhibition of G protein-mediated pathways using RGS domains.

  2004cites this paper
• The targetable G protein proteome: where is the next generation of drug targets?

  2004cites this paper
• Proximal Events in Signaling by Plasma Membrane Estrogen Receptors*

  2003cites this paper
• Gene and Protein Domain-Specific Patterns of Genetic Variability Within the G-Protein Coupled Receptor Superfamily

  2003cites this paper
• Principles: extending the utility of [35S]GTP gamma S binding assays.

  2003cites this paper
• Exocytosis in Alveolar Epithelial Cells

  2003cites this paper
• The GTP-binding protein RhoA mediates Na,K-ATPase exocytosis in alveolar epithelial cells.

  2003cites this paper
• Dancing with Multiple Partners

  2002cites this paper
• Cellular Physiology Cellular Physiology Cellular Physiology Cellular Physiology Cellular Physiology Impaired M 3 Muscarinic Acetylcholine Receptor Signal Transduction through Blockade of Binding of Multiple Proteins to Its Third Intracellular Loop Key Words Third Intracellular Loop @bullet Muscarini

  year unknowncites this paper