Characterization of a Novel Serine/Threonine Kinase Associated with Nuclear Bodies*

A novel protein kinase, Mx-interacting protein kinase (PKM), has been identified in a yeast two-hybrid screen for interaction partners of human MxA, an interferon-induced GTPase with antiviral activity against several RNA viruses. A highly conserved protein kinase domain is present in the N-terminal moiety of PKM, whereas an Mx interaction domain overlaps with C-terminal PEST sequences. PKM has a molecular weight of about 127,000 and exhibits high sequence homology to members of a recently described family of homeodomain-interacting protein kinases. Recombinant PKM has serine/threonine kinase activity that is abolished by a single amino acid substitution in the ATP binding domain (K221W). PKM catalyzes autophosphorylation and phosphorylation of various cellular and viral proteins. PKM is expressed constitutively and colocalizes with the interferon-inducible Sp100 protein and murine Mx1 in discrete nuclear structures known as nuclear bodies.

• Publication year

  2000

• Venue

  Journal of Biological Chemistry

• Publication date

  2000-03-10

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/jbc.275.10.7373PMID 10702310
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• GTP-bound Human MxA Protein Interacts with the Nucleocapsids of Thogoto Virus (Orthomyxoviridae)*

  1999cited by this paper
• Covalent modification of the homeodomain-interacting protein kinase 2 (HIPK2) by the ubiquitin-like protein SUMO-1.

  1999cited by this paper
• Nuclear domain 10, the site of DNA virus transcription and replication

  1998cited by this paper
• Mx proteins: mediators of innate resistance to RNA viruses.

  1998cited by this paper
• MxA GTPase: oligomerization and GTP-dependent interaction with viral RNP target structures.

  1998cited by this paper
• Activation of androgen receptor function by a novel nuclear protein kinase.

  1998cited by this paper
• Homeodomain-interacting Protein Kinases, a Novel Family of Co-repressors for Homeodomain Transcription Factors*

  1998cited by this paper
• Conjugation with the ubiquitin‐related modifier SUMO‐1 regulates the partitioning of PML within the nucleus

  1998cited by this paper
• Structure and function in the nucleus.

  1998cited by this paper
• Resistance to Virus Infection Conferred by the Interferon-Induced Promyelocytic Leukemia Protein

  1998cited by this paper
• A classical bipartite nuclear localization signal on Thogoto and influenza A virus nucleoproteins.

  1998cited by this paper
• Human MxA Protein Confers Resistance to Semliki Forest Virus and Inhibits the Amplification of a Semliki Forest Virus-Based Replicon in the Absence of Viral Structural Proteins

  1998cited by this paper
• Nuclear dots: actors on many stages.

  1997cited by this paper
• SUMO-1: wrestling with a new ubiquitin-related modifier.

  1997cited by this paper
• Dominant-negative mutants of human MxA protein: domains in the carboxy-terminal moiety are important for oligomerization and antiviral activity

  1997cited by this paper
• Identification and sequence of human PKY, a putative kinase with increased expression in multidrug-resistant cells, with homology to yeast protein kinase Yak1.

  1997cited by this paper
• Isolation of human and murine homologues of the Drosophila minibrain gene: human homologue maps to 21q22.2 in the Down syndrome "critical region".

  1996cited by this paper
• Two versatile eukaryotic vectors permitting epitope tagging, radiolabelling and nuclear localisation of expressed proteins.

  1996cited by this paper
• Identification of the structural and functional human homolog of the yeast ubiquitin conjugating enzyme UBC9.

  1996cited by this paper
• Two versatile eukaryotic expression vectors permitting epitope tagging, radiolabelling and nuclear localisation of expressed proteins

  1996cited by this paper
• Dyrk, a Dual Specificity Protein Kinase with Unique Structural Features Whose Activity Is Dependent on Tyrosine Residues between Subdomains VII and VIII (*)

  1996cited by this paper
• Cloning of a human homolog of the Drosophila minibrain/rat Dyrk gene from "the Down syndrome critical region" of chromosome 21.

  1996cited by this paper
• Nucleoprotein viral RNA and mRNA of Thogoto virus: a novel "cap-stealing" mechanism in tick-borne orthomyxoviruses?

  1996cited by this paper
• The acute promyelocytic leukaemia-associated PML gene is induced by interferon.

  1995cited by this paper
• Induction of the PML protein by interferons in normal and APL cells.

  1995cited by this paper
• Cell type-specific MxA-mediated inhibition of measles virus transcription in human brain cells

  1994cited by this paper
• A novel macromolecular structure is a target of the promyelocyte-retinoic acid receptor oncoprotein.

  1994cited by this paper
• Rapid amplification of complementary DNA ends for generation of full-length complementary DNAs: thermal RACE.

  1993cited by this paper
• Function of the mouse Mx1 protein is inhibited by overexpression of the PB2 protein of influenza virus.

  1993cited by this paper
• Modification of discrete nuclear domains induced by herpes simplex virus type 1 immediate early gene 1 product (ICP0).

  1993cited by this paper
• A functional GTP-binding motif is necessary for antiviral activity of Mx proteins

  1993cited by this paper
• Mx proteins: GTPases with antiviral activity.

  1993cited by this paper
• IFN enhance expression of Sp100, an autoantigen in primary biliary cirrhosis.

  1992cited by this paper
• Dual-specificity protein kinases: will any hydroxyl do?

  1992cited by this paper
• Cloning and expression of the vesicular stomatitis virus phosphoprotein gene in Escherichia coli: analysis of phosphorylation status versus transcriptional activity

  1991cited by this paper
• Raf-1 protein kinase is required for growth of induced NIH/3T3 cells

  1991cited by this paper
• Phosphopeptide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates.

  1991cited by this paper
• Overview of manual and automated DNA sequencing by the dideoxy chain termination method

  1991cited by this paper
• cDNA structures and regulation of two interferon-induced human Mx proteins

  1989cited by this paper
• Differential Phosphorylation of the Nucleoprotein of Influenza A Viruses

  1989cited by this paper
• Differential phosphorylation of the nucleoprotein of influenza A viruses.

  1988cited by this paper
• Identification of a second interferon-regulated murine Mx gene

  1988cited by this paper
• IFN-alpha induced human 78 kD protein: purification and homologies with the mouse Mx protein, production of monoclonal antibodies, and potentiation effect of IFN-gamma.

  1987cited by this paper
• IFN-αInduced Human 78 kD Protein: Purification and Homologies with the Mouse Mx Protein, Production of Monoclonal Antibodies, and Potentiation Effect of IFN-γ

  1987cited by this paper
• Amino acid sequences common to rapidly degraded proteins: the PEST hypothesis.

  1986cited by this paper
• Mx protein: constitutive expression in 3T3 cells transformed with cloned Mx cDNA confers selective resistance to influenza virus.

  1986cited by this paper
• Eukaryotic transient-expression system based on recombinant vaccinia virus that synthesizes bacteriophage T7 RNA polymerase.

  1986cited by this paper
• Interferon-induced protein Mx accumulates in nuclei of mouse cells expressing resistance to influenza viruses.

  1985cited by this paper
• T98G: An anchorage‐independent human tumor cell line that exhibits stationary phase G1 arrest in vitro

  1979cited by this paper
• Isolation of Thogoto virus from Rhipicephalus bursa ticks in western Sicily.

  1972cited by this paper

• Recent advances in the development of HIPK2 inhibitors as anti-renal fibrosis agents.

  2023cites this paper
• The important roles of protein SUMOylation in the occurrence and development of leukemia and clinical implications

  2020cites this paper
• Functional impairment of the HIPK2 small ubiquitin-like modifier (SUMO)-interacting motif in acute myeloid leukemia.

  2019cites this paper
• Functional impairment of the HIPK 2 small ubiquitin-like modifier ( SUMO )-interacting motif in acute myeloid leukemia

  2019cites this paper
• Pandemic Influenza A Viruses Escape from Restriction by Human MxA through Adaptive Mutations in the Nucleoprotein

  2013cites this paper
• Papel da proteína Mx1 na resposta a interferons e no processo neoplásico

  2011influential citation
• 同源结构域相互作用蛋白激酶2最新研究进展及其应用

  2011cites this paper
• Role of the SUMO-interacting motif in HIPK2 targeting to the PML nuclear bodies and regulation of p53.

  2011cites this paper
• Interferon-induced Mx proteins in antiviral host defense.

  2007cites this paper
• The Mx GTPase family of interferon-induced antiviral proteins.

  2007cites this paper
• Funktionelle Analyse des Regulatorproteins ICP0 von Herpes Simplex Virus Typ 2

  2006cites this paper
• The interferon-inducible GTPases.

  2006cites this paper
• Molecular cloning of the cDNA of canine homeodomain-interacting protein kinase 2.

  2005cites this paper
• HIPK 2-Induced Cell Growth Arrest Interacts with HIPK 2 and Antagonizes US 11 of Herpes Simplex Virus Type 1

  2004cites this paper
• US11 of Herpes Simplex Virus Type 1 Interacts with HIPK2 and Antagonizes HIPK2-Induced Cell Growth Arrest

  2004influential citation
• Mx1 GTPase accumulates in distinct nuclear domains and inhibits influenza A virus in cells that lack promyelocytic leukaemia protein nuclear bodies.

  2004influential citation
• Homeodomain-interacting protein kinase-2 activity and p53 phosphorylation are critical events for cisplatin-mediated apoptosis.

  2004cites this paper
• CHAPTER 249 – Mx Proteins: High Molecular Weight GTPases with Antiviral Activity

  2003cites this paper
• The homeodomain-interacting kinase PKM (HIPK-2) modifies ND10 through both its kinase domain and a SUMO-1 interaction motif and alters the posttranslational modification of PML.

  2003cites this paper
• DYRK1A accumulates in splicing speckles through a novel targeting signal and induces speckle disassembly

  2003influential citation
• HIPK2 associates with RanBPM.

  2002cites this paper
• Regulation of p53 activity by its interaction with homeodomain-interacting protein kinase-2

  2002cites this paper
• Identification and Characterization of HIPK2 Interacting with p73 and Modulating Functions of the p53 Familyin Vivo *

  2002cites this paper
• Transcriptional regulation in acute promyelocytic leukemia

  2001cites this paper
• Cellular proteins localized at and interacting within ND10/PML nuclear bodies/PODs suggest functions of a nuclear depot

  2001cites this paper
• Interferon-induced antiviral Mx1 GTPase is associated with components of the SUMO-1 system and promyelocytic leukemia protein nuclear bodies.

  2001cites this paper
• Isolation and characterization of cDNAs for the protein kinase HIPK2.

  2001cites this paper
• HIPK2 overexpression leads to stabilization of p53 protein and increased p53 transcriptional activity by decreasing Mdm2 protein levels

  2001influential citation
• Replication but Not Transcription of Simian Virus 40 DNA Is Dependent on Nuclear Domain 10

  2000cites this paper
• Human homeodomain-interacting protein kinase-2 (HIPK2) is a member of the DYRK family of protein kinases and maps to chromosome 7q32-q34.

  2000influential citation
• Covalent modification of p73alpha by SUMO-1. Two-hybrid screening with p73 identifies novel SUMO-1-interacting proteins and a SUMO-1 interaction motif.

  2000cites this paper
• The serine/threonine kinase HIPK2 interacts with TRADD, but not with CD95 or TNF-R1 in 293T cells.

  2000cites this paper