Substitution of lysine with glutamic acid at position 193 in bovine CYP11A1 significantly affects protein oligomerization and solubility but not enzymatic activity.

No abstract is available for this paper.

• Publication year

  2011

• Venue

  Biochimica et Biophysica Acta

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.bbapap.2010.06.002PMID 20538078
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Cytochrome P450 and Steroid Hormone Biosynthesis

  2007cited by this paper
• Protein phosphorylation and intermolecular electron transfer: a joint experimental and computational study of a hormone biosynthesis pathway.

  2007cited by this paper
• The ubiquitous roles of cytochrome P450 proteins

  2007cited by this paper
• Synthesis of 3d metallic single-molecule magnets

  2006cited by this paper
• Cytochromes P450 as versatile biocatalysts.

  2006cited by this paper
• Design of an Escherichia coli system for whole cell mediated steroid synthesis and molecular evolution of steroid hydroxylases.

  2006cited by this paper
• A novel pathway for sequential transformation of 7-dehydrocholesterol and expression of the P450scc system in mammalian skin.

  2004cited by this paper
• Putative F-G loop is involved in association with the membrane in P450scc (P450 11A1).

  2004cited by this paper
• The F-G loop region of cytochrome P450scc (CYP11A1) interacts with the phospholipid membrane.

  2003cited by this paper
• Probing the interaction of bovine cytochrome P450scc (CYP11A1) with adrenodoxin: evaluating site-directed mutations by molecular modeling.

  2002cited by this paper
• Laboratory evolution of a soluble, self-sufficient, highly active alkane hydroxylase

  2002cited by this paper
• Covalently crosslinked complexes of bovine adrenodoxin with adrenodoxin reductase and cytochrome P450scc. Mass spectrometry and Edman degradation of complexes of the steroidogenic hydroxylase system.

  2001cited by this paper
• Adrenodoxin: Structure, stability, and electron transfer properties

  2000cited by this paper
• Engineering Microsomal Cytochrome P450 2C5 to Be a Soluble, Monomeric Enzyme

  2000cited by this paper
• A simple in vivo assay for increased protein solubility

  1999cited by this paper
• Expression of catalytically active human cytochrome p450scc in Escherichia coli and mutagenesis of isoleucine-462.

  1998cited by this paper
• Self-sufficient biosynthesis of pregnenolone and progesterone in engineered yeast

  1998cited by this paper
• Chaperone Coexpression Plasmids: Differential and Synergistic Roles of DnaK-DnaJ-GrpE and GroEL-GroES in Assisting Folding of an Allergen of Japanese Cedar Pollen, Cryj2, inEscherichia coli

  1998cited by this paper
• Advances in microbial steroid biotransformation.

  1997cited by this paper
• VMD: visual molecular dynamics.

  1996cited by this paper
• New applications of bacterial systems to problems in toxicology.

  1996cited by this paper
• Rotation of cytochrome P450SCC (CYP11A1) in proteoliposomes studied by delayed fluorescence depolarization.

  1993cited by this paper
• Direct expression of adrenodoxin reductase in Escherichia coli and the functional characterization.

  1993cited by this paper
• Identification by site-directed mutagenesis of two lysine residues in cholesterol side chain cleavage cytochrome P450 that are essential for adrenodoxin binding.

  1992cited by this paper
• Expression of bovine adrenodoxin in E. coli and site-directed mutagenesis of /2 Fe-2S/ cluster ligands.

  1992cited by this paper
• Expression of functional bovine cholesterol side chain cleavage cytochrome P450 (P450scc) in Escherichia coli.

  1991cited by this paper
• Sensitive assay of cytochrome P450scc activity by high-performance liquid chromatography.

  1989cited by this paper
• Cross-linking studies of adrenocortical cytochrome P-450scc. Evidence for a covalent complex with adrenodoxin and localization of the adrenodoxin-binding domain.

  1985cited by this paper
• Properties of crystalline reduced nicotinamide adenine dinucleotide phosphate-adrenodoxin reductase from bovine adrenocortical mitochonria. I. Physicochemical properties of holo- and apo-NADPH-adrenodoxin reductase and interaction between non-heme iron proteins and the reductase.

  1976cited by this paper
• THE CARBON MONOXIDE-BINDING PIGMENT OF LIVER MICROSOMES. I. EVIDENCE FOR ITS HEMOPROTEIN NATURE.

  1964cited by this paper

• Optimizing protein folding in prokaryotes: Strategies to enhance soluble expression of recombinant proteins.

  2025cites this paper
• Comparative biochemical characterization of mammalian-derived CYP11A1s with cholesterol side-chain cleavage activities.

  2023cites this paper
• Directed Evolution of Soluble α-1,2-Fucosyltransferase Using Kanamycin Resistance Protein as a Phenotypic Reporter for Efficient Production of 2'-Fucosyllactose

  2022cites this paper
• Light-driven progesterone production by InP–(M. neoaurum) biohybrid system

  2022cites this paper
• An antibacterial novel peptide based on cecropin and MAP-27: Design and characterization

  2022cites this paper
• Underestimated reactions and regulation patterns of adrenal cytochromes P450.

  2021cites this paper
• The Key Role of E418 Carboxyl Group in the Formation of Nt.BspD6I Nickase Active Site: Structural and Functional Properties of Nt.BspD6I E418A Mutant.

  2020cites this paper
• Selection and screening strategies in directed evolution to improve protein stability

  2019cites this paper
• Functionalized poly(3-hydroxybutyric acid) bodies as new in vitro biocatalysts.

  2018cites this paper
• In Vitro and In Vivo characterization of CYP11A1, CYP17A1 and cChloramphenicol acetyltransferase-dependent steroid conversion

  2015cites this paper
• 2β- and 16β-hydroxylase activity of CYP11A1 and direct stimulatory effect of estrogens on pregnenolone formation.

  2015cites this paper
• IN VITRO AND IN VIVO CHARACTERIZATION OF CYP 11 A 1 , CYP 17 A 1 AND CHLORAMPHENICOL ACETYLTRANSFERASE-DEPENDENT STEROID CONVERSION

  2015cites this paper
• Functionalized PHB granules provide the basis for the efficient side-chain cleavage of cholesterol and analogs in recombinant Bacillus megaterium

  2015cites this paper
• Dehydroepiandrosterone Sulfate (DHEAS) Stimulates the First Step in the Biosynthesis of Steroid Hormones

  2014cites this paper
• Structural and Functional Analysis of Novel Human Cytochrome c Targets in Apoptosis*

  2014cites this paper
• New Arabidopsis thaliana Cytochrome c Partners: A Look Into the Elusive Role of Cytochrome c in Programmed Cell Death in Plants*

  2013cites this paper
• Integrating the protein and metabolic engineering toolkits for next-generation chemical biosynthesis.

  2013cites this paper