Engineering, characterization and phage display of hepatitis C virus NS3 protease and NS4A cofactor peptide as a single-chain protein.

No abstract is available for this paper.

• Publication year

  1998

• Venue

  Protein Engineering

• Publication date

  1998-12-01

• Fields of study

  Biology, Medicine, Engineering

• Identifiers
  DOI 10.1093/PROTEIN/11.12.1257PMID 9930676
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Design of selective eglin inhibitors of HCV NS3 proteinase.

  1998cited by this paper
• Complex of NS3 protease and NS4A peptide of BK strain hepatitis C virus: A 2.2 Å resolution structure in a hexagonal crystal form

  1998cited by this paper
• Rational design and functional expression of a constitutively active single-chain NS4A-NS3 proteinase.

  1998cited by this paper
• Construction, expression, and characterization of a novel fully activated recombinant single‐chain hepatitis C virus protease

  1998cited by this paper
• Serine protease of hepatitis C virus expressed in insect cells as the NS3/4A complex.

  1998cited by this paper
• Expression of a hepatitis C virus NS3 protease-NS4A fusion protein in Escherichia coli.

  1998cited by this paper
• Characterisation of an HCV NS3/NS4A proteinase fusion protein expressed in E.coli using synthetic peptide substrates.

  1997cited by this paper
• Protein folding in the bacterial periplasm

  1997cited by this paper
• Probing the substrate specificity of hepatitis C virus NS3 serine protease by using synthetic peptides

  1997cited by this paper
• Substrate Specificity of the Hepatitis C Virus Serine Protease NS3*

  1997cited by this paper
• Chimeric Sindbis viruses dependent on the NS3 protease of hepatitis C virus

  1997cited by this paper
• Expression of highly active recombinant NS3 protease domain of hepatitis C virus in E. coli

  1997cited by this paper
• Affinity selection of a camelized V(H) domain antibody inhibitor of hepatitis C virus NS3 protease.

  1997cited by this paper
• Mechanistic role of an NS4A peptide cofactor with the truncated NS3 protease of hepatitis C virus: elucidation of the NS4A stimulatory effect via kinetic analysis and inhibitor mapping.

  1997cited by this paper
• Characterization of engineered hepatitis C virus NS3 protease inhibitors affinity selected from human pancreatic secretory trypsin inhibitor and minibody repertoires

  1997cited by this paper
• The crystal structure of hepatitis C virus NS3 proteinase reveals a trypsin-like fold and a structural zinc binding site.

  1996cited by this paper
• Crystal structure of the hepatitis C virus NS3 protease domain complexed with a synthetic NS4A cofactor peptide.

  1996cited by this paper
• Generation of a novel poliovirus with a requirement of hepatitis C virus protease NS3 activity.

  1996cited by this paper
• A central hydrophobic domain of the hepatitis C virus NS4A protein is necessary and sufficient for the activation of the NS3 protease.

  1996cited by this paper
• Activity of purified hepatitis C virus protease NS3 on peptide substrates

  1996cited by this paper
• A one-step procedure for biotinylation and chemical cross-linking of lymphocyte surface and intracellular membrane-associated molecules.

  1995cited by this paper
• Hepatitis C virus-encoded nonstructural protein NS4A has versatile functions in viral protein processing

  1995cited by this paper
• Selection of beta-lactamase on filamentous bacteriophage by catalytic activity.

  1994cited by this paper
• Phage display of enzymes and in vitro selection for catalytic activity

  1994cited by this paper
• The affinity‐selection of a minibody polypeptide inhibitor of human interleukin‐6.

  1994cited by this paper
• NS3 is a serine protease required for processing of hepatitis C virus polyprotein

  1993cited by this paper
• Characterization of the hepatitis C virus-encoded serine proteinase: determination of proteinase-dependent polyprotein cleavage sites

  1993cited by this paper
• Trypsin display on the surface of bacteriophage.

  1993cited by this paper
• Diagnosis of hepatitis C virus (HCV) infection using an immunodominant chimeric polyprotein to capture circulating antibodies: reevaluation of the role of HCV in liver disease.

  1992cited by this paper
• Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains.

  1991cited by this paper
• Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome.

  1989cited by this paper
• Specificity of the immunoadsorbent used for large-scale recovery of interferon alpha-2a.

  1987cited by this paper
• Structure of the elastase-cathepsin G inhibitor of the leech Hirudo medicinalis.

  1980cited by this paper

• Mechanistic and Kinetic Insights into the Acylation Reaction of Hepatitis C Virus NS3/NS4A Serine Protease with NS4B/5A Substrate

  2025cites this paper
• Theoretical Study of the Free Energy Surface and Kinetics of the Hepatitis C Virus NS3/NS4A Serine Protease Reaction with the NS5A/5B Substrate. Does the Generally Accepted Tetrahedral Intermediate Really Exist?

  2015cites this paper
• HelicaseSeparation of Protease and Swine Fever Virus NS3 Leads to a Autocatalytic Cleavage within Classical

  2014cites this paper
• Autocatalytic Cleavage within Classical Swine Fever Virus NS3 Leads to a Functional Separation of Protease and Helicase

  2013cites this paper
• High Affinity Peptide Inhibitors of the Hepatitis C Virus NS3-4A Protease Refractory to Common Resistant Mutants

  2012cites this paper
• A new method for induced fit docking (GENIUS) and its application to virtual screening of novel HCV NS3-4A protease inhibitors.

  2011cites this paper
• Engineered Toxins “Zymoxins” Are Activated by the HCV NS3 Protease by Removal of an Inhibitory Protein Domain

  2011cites this paper
• A comparative QM/MM study of the reaction mechanism of the Hepatitis C virus NS3/NS4A protease with the three main natural substrates NS5A/5B, NS4B/5A and NS4A/4B.

  2010cites this paper
• Fluorescence Resonance Energy Transfer-Based Assay for Characterization of Hepatitis C Virus NS3-4A Protease Activity in Live Cells (cid:1)

  2009cites this paper
• Conformational changes in redox pairs of protein structures

  2009cites this paper
• Use of the fused NS4A peptide-NS3 protease domain to study the importance of the helicase domain for protease inhibitor binding to hepatitis C virus NS3-NS4A.

  2009cites this paper
• Identification of amino acid variants in the hepatitis C virus non-structural protein 4A.

  2009cites this paper
• A wide range of NS3/4A protease catalytic efficiencies in HCV-infected individuals.

  2008cites this paper
• Fluorescence Resonance Energy Transfer-Based Assay for Characterization of Hepatitis C Virus NS3-4A Protease Activity in Live Cells

  2008cites this paper
• Antiviral suppression vs restoration of RIG-I signaling by hepatitis C protease and polymerase inhibitors.

  2008cites this paper
• Development of a cell-based assay for monitoring hepatitis C virus ns3/4a protease activity.

  2008cites this paper
• Differential Requirements of NS 4 A for Internal NS 3 Cleavage and Polyprotein Processing of Hepatitis C Virus

  2007influential citation
• Differential Requirements of NS4A for Internal NS3 Cleavage and Polyprotein Processing of Hepatitis C Virus

  2007influential citation
• A quantum mechanics/molecular mechanics study of the reaction mechanism of the hepatitis C virus NS3 protease with the NS5A/5B substrate

  2006cites this paper
• Humoral immune response to recombinant viral NS3 protein in patients with hepatitis C

  2005cites this paper
• Inhibition of hepatitis C virus NS3-mediated cell transformation by recombinant intracellular antibodies.

  2004cites this paper
• Enzyme Engineering by Phage Display

  2003cites this paper
• Genetic Screen for Monitoring Hepatitis C Virus NS3 Serine Protease Activity

  2003cites this paper
• A novel high throughput screening assay for HCV NS3 serine protease inhibitors.

  2003cites this paper
• A personal account of the role of peptide research in drug discovery: the case of hepatitis C 1

  2001cites this paper
• Isolation and Characterization of Monoclonal Antibodies That Inhibit Hepatitis C Virus NS3 Protease

  2000cites this paper
• Proteases of the Hepatitis C Virus

  1999cites this paper