Automated Extraction and Quantification of Human Cytomegalovirus DNA in Whole Blood by Real-Time PCR Assay

No abstract is available for this paper.

• Publication year

  2003

• Venue

  Journal of Clinical Microbiology

• Publication date

  2003-08-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1128/JCM.41.8.3840-3845.2003PMID 12904398PMCID PMC179853
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Quantitation of human cytomegalovirus in recipients of solid organ transplants by real‐time quantitative PCR and pp65 antigenemia

  2003cited by this paper
• The clinical use of various blood compartments for cytomegalovirus (CMV) DNA quantitation in transplant recipients with CMV disease.

  2002cited by this paper
• Comparative Quantitation of Cytomegalovirus (CMV) DNA in Solid Organ Transplant Recipients with CMV Infection by Using Two High-Throughput Automated Systems

  2001cited by this paper
• Quantification of Human Cytomegalovirus DNA by Real-Time PCR

  2001cited by this paper
• Relationship of cytomegalovirus viral load in blood to pneumonitis in lung transplant recipients.

  2001cited by this paper
• Evaluation of CMV viral load using TaqMan CMV quantitative PCR and comparison with CMV antigenemia in heart and lung transplant recipients.

  2001cited by this paper
• Cytomegalovirus diagnosis in renal and bone marrow transplant recipients: the impact of molecular assays.

  2001cited by this paper
• Quantification of Human Cytomegalovirus DNA in Bone Marrow Transplant Recipients by Real-Time PCR

  2001cited by this paper
• Comparison of Quantitative and Qualitative PCR Assays for Cytomegalovirus DNA in Plasma

  2001cited by this paper
• Comparison of plasma polymerase chain reaction and pp65-antigenemia assay in the quantification of cytomegalovirus in liver and kidney transplant patients.

  2001cited by this paper
• Comparison of Two Commercial Methods for Measurement of Cytomegalovirus Load in Blood Samples after Renal Transplantation

  2000cited by this paper
• Prediction and diagnosis of cytomegalovirus disease in renal transplant recipients using qualitative and quantitative polymerase chain reaction.

  2000cited by this paper
• Quantitative Analysis of Cytomegalovirus (CMV) Viremia Using the pp65 Antigenemia Assay and the COBAS AMPLICOR CMV MONITOR PCR Test after Blood and Marrow Allogeneic Transplantation

  2000cited by this paper
• Comparison of PCR, Antigenemia Assay, and Rapid Blood Culture for Detection and Prevention of Cytomegalovirus Disease after Lung Transplantation

  2000cited by this paper
• New molecular assays to predict occurrence of cytomegalovirus disease in renal transplant recipients.

  2000cited by this paper
• Real-Time Automated PCR for Early Diagnosis and Monitoring of Cytomegalovirus Infection after Bone Marrow Transplantation

  2000cited by this paper
• Clinical utility of quantitative cytomegalovirus viral load determination for predicting cytomegalovirus disease in liver transplant recipients.

  1999cited by this paper
• Comparative evaluation of the cytomegalovirus DNA load in polymorphonuclear leukocytes and plasma of human immunodeficiency virus-infected subjects.

  1998cited by this paper
• Use of Laboratory Assays To Predict Cytomegalovirus Disease in Renal Transplant Recipients

  1998cited by this paper
• Quantitative polymerase chain reaction to predict occurrence of symptomatic cytomegalovirus infection and assess response to ganciclovir therapy in renal transplant recipients.

  1998cited by this paper
• Comparison of nested PCR for detection of DNA in plasma with pp65 leukocytic antigenemia procedure for diagnosis of human cytomegalovirus infection

  1994cited by this paper

• Human cytomegalovirus UL23 exploits PD-L1 inhibitory signaling pathway to evade T cell-mediated cytotoxicity

  2024cites this paper
• Comparative analyses of DNA extraction methods for whole blood quantification of HCMV DNAemia in patients with hematological diseases: false negative cases in manual method

  2023cites this paper
• MOLECULAR DETECTION OF CYTOMEGALOVIRUS (CMV) AND PRESENCE OF BACTERIAL INFECTION IN ABORTED WOMEN

  2020cites this paper
• High tacrolimus intra-patient variability is associated with graft rejection, and de novo donor-specific antibodies occurrence after liver transplantation

  2018cites this paper
• Strategies to control human cytomegalovirus infection in adult hematopoietic stem cell transplant recipients.

  2016cites this paper
• Nucleic Acid Amplification Techniques in the Diagnosis of Infectious Diseases: The Way Forward

  2016cites this paper
• Comparison of two molecular assays for detection of cytomegalovirus DNA in whole blood 2 and plasma samples from transplant recipients 3

  2016cites this paper
• Comparison of two molecular assays for detection of cytomegalovirus DNA in whole blood and plasma samples from transplant recipients.

  2016cites this paper
• Performance of a completely automated system for monitoring CMV DNA in plasma.

  2016cites this paper
• Comparison of two molecular assays for detection of cytomegalovirus DNA in whole blood and plasma samples from transplant recipients.

  2016cites this paper
• Risk Factors of Pneumocystis Pneumonia in Solid Organ Recipients in the Era of the Common Use of Posttransplantation Prophylaxis

  2015cites this paper
• Correlation between systemic lupus erythematosus and cytomegalovirus infection detected by different methods

  2015cites this paper
• Prophylaxis versus pre‐emptive treatment for prevention of cytomegalovirus infection in CMV‐seropositive orthotopic liver‐transplant recipients

  2015cites this paper
• Determinants of Cytomegalovirus Infection Outcomes in Solid Organ Transplant Recipients

  2015cites this paper
• Performance of a new rapid test for detecting anti-hepatitis E virus immunoglobulin M in immunocompetent and immunocompromised patients.

  2015cites this paper
• CMV management in lung transplant recipients

  2014cites this paper
• Molecular Detection of Human Cytomegalovirus genes in infertile and breast cancer women in Baghdad province

  2014cites this paper
• Comparison of human cytomegalovirus load in whole blood and plasma samples of transplant recipient patients

  2014cites this paper
• Quantification of cytomegalovirus viral load

  2014cites this paper
• An Association between BK Virus Replication in Bone Marrow and Cytopenia in Kidney-Transplant Recipients

  2014influential citation
• Prospective monitoring of cytomegalovirus, Epstein-Barr virus, BK virus, and JC virus infections on belatacept therapy after a kidney transplant.

  2014cites this paper
• Prospective evaluation of a new automated nucleic acid extraction system using routine clinical respiratory specimens†

  2012cites this paper
• Comparison of quantitative HCMV DNAemia in whole blood and leukocytes, and Real-Time PCR in a BMT patient

  2012cites this paper
• Quantitation of human cytomegalovirus DNA in plasma using an affordable in-house qPCR assay.

  2012cites this paper
• A new highly automated extraction system for quantitative real-time PCRs from whole blood samples: routine monitoring of opportunistic infections in immunosuppressed patients.

  2012cites this paper
• Low risk of hepatitis E virus reactivation after haematopoietic stem cell transplantation.

  2012cites this paper
• Severe steroid-resistant thrombocytopenia secondary to cytomegalovirus infection in an immunocompetent adult.

  2012cites this paper
• State of the Art and Trends in Cytomegalovirus Diagnostics

  2012cites this paper
• JC virus DNA in the peripheral blood of renal transplant patients: A 1‐year prospective follow‐up in France

  2011influential citation
• Management of human cytomegalovirus infection in transplantation: validation of virologic cut-offs for preemptive therapy and immunological cut-offs for protection.

  2011cites this paper
• Comparison of 2 highly automated nucleic acid extraction systems for quantitation of human cytomegalovirus in whole blood.

  2011cites this paper
• The Clinical Utility of Whole Blood Versus Plasma Cytomegalovirus Viral Load Assays for Monitoring Therapeutic Response

  2011cites this paper
• Expression of O6-methylguanine-DNA methyltransferase in childhood medulloblastoma

  2011cites this paper
• Comparison of real-time PCR and pp65 antigen assays for monitoring the development of Cytomegalovirus disease in recipients of solid organ and bone marrow transplants.

  2011cites this paper
• Comparison of real-time PCR and pp 65 antigen assays for monitoring the development of Cytomegalovirus disease in recipients of solid organ and bone marrow transplants

  2011cites this paper
• Clinical evaluation of a fully automated CMV PCR assay.

  2011cites this paper
• Expression of O 6 -methylguanine-DNA methyltransferase in childhood medulloblastoma Denis FaoroAndreO. von BuerenTarek ShalabyDavide Sciuscio • Marie-Louise HurlimannLucia ArnoldNicolas U. GerberJohannes Haybaeck • Michel MittelbronnStefan RutkowskiMonika HegiMichael A. Grotzer

  2011cites this paper
• Molecular detection of herpesviruses.

  2011cites this paper
• Monitoring human cytomegalovirus infection with nested PCR: comparison of positive rates in plasma and leukocytes and with quantitative PCR

  2010cites this paper
• Rapid quantitation of cytomegalovirus DNA in whole blood by a new molecular assay based on automated sample preparation and real-time PCR

  2010cites this paper
• Multicenter evaluation of prototype real‐time PCR assays for Epstein‐Barr virus and cytomegalovirus DNA in whole blood samples from transplant recipients

  2010cites this paper
• [Cytomegalovirus effects in solid organ transplantation and the role of antiviral prophylaxis].

  2010cites this paper
• Heat denaturation increases the sensitivity of the cytomegalovirus loop‐mediated isothermal amplification method

  2010cites this paper
• [Comparison between the LightCycler CMV Quant Kit (Roche Diagnostics) with a standardized in-house Taqman assay for cytomegalovirus blood viral load quantification].

  2010cites this paper
• Cellular normalization of viral DNA loads on whole blood improves the clinical management of cytomegalovirus or Epstein Barr virus infections in the setting of pre‐emptive therapy

  2009cites this paper
• Detection of Human Cytomegalovirus (HCMV) and Porcine Endogenous Retrovirus (PERV) with One Step Extraction Method

  2009cites this paper
• Interferon gamma 13-CA-repeat homozygous genotype and a low proportion of CD4(+) lymphocytes are independent risk factors for cytomegalovirus reactivation with a high number of copies in hematopoietic stem cell transplantation recipients.

  2009cites this paper
• Decreased risk of congenital cytomegalovirus infection in children born to HIV-1-infected mothers in the era of highly active antiretroviral therapy.

  2009cites this paper
• Multicenter quality control study for human cytomegalovirus DNAemia quantification.

  2009cites this paper
• Analysis of mixed infections by multiple genotypes of human cytomegalovirus in immunocompromised patients

  2009cites this paper
• Direct genotyping of cytomegalovirus envelope glycoproteins from toddler's saliva samples.

  2009cites this paper
• Different expression of human herpesvirus-6 (HHV-6) load in whole blood may have a significant impact on the diagnosis of active infection.

  2009cites this paper
• Comparative Evaluation of a Commercially Available Automated System for Extraction of Viral DNA from Whole Blood: Application to Monitoring of Epstein-Barr Virus and Cytomegalovirus Load

  2009cites this paper
• Management of human cytomegalovirus infection in transplant recipients by the pre-emptive therapy approach

  2009cites this paper
• Comparison of two highly automated DNA extraction systems for quantifying Epstein-Barr virus in whole blood.

  2008cites this paper
• Evaluation of an automated extraction system in combination with Affigene CMV Trender for CMV DNA quantitative determination: comparison with nested PCR and pp65 antigen test.

  2008cites this paper
• Detection of Cytomegalovirus (CMV) DNA in EDTA Whole-Blood Samples: Evaluation of the Quantitative artus CMV LightCycler PCR Kit in Conjunction with Automated Sample Preparation

  2008cites this paper
• Cytomegalovirus prophylaxis with valganciclovir in cytomegalovirus‐seropositive kidney‐transplant patients

  2008cites this paper
• Detection of CMV DNA: is EDTA whole blood superior to EDTA plasma?

  2008cites this paper
• Evaluation of a novel real-time PCR system for cytomegalovirus DNA quantitation on whole blood and correlation with pp65-antigen test in guiding pre-emptive antiviral treatment.

  2008cites this paper
• Predictive factors for cytomegalovirus reactivation in cytomegalovirus‐seropositive kidney‐transplant patients

  2008cites this paper
• Identification of a highly conserved region in the human cytomegalovirus glycoprotein H gene and design of molecular diagnostic methods targeting the region.

  2008cites this paper
• Applications of Real-Time PCR in Clinical Microbiology

  2008cites this paper
• Monitoring of Cytomegalovirus Reactivation in Bone Marrow Transplant Recipients by Real-time PCR

  2008cites this paper
• Human herpesvirus-6 (HHV-6) DNA in plasma reflects the presence of infected blood cells rather than circulating viral particles.

  2007cites this paper
• Simultaneous Quantification of Epstein-Barr Virus, Cytomegalovirus, and Human Herpesvirus 6 DNA in Samples from Transplant Recipients by Multiplex Real-Time PCR Assay

  2007cites this paper
• CMV detection and monitoring in Bone Marrow Transplant (BMT) recipients by Real-Time PCR (RQ-PCR)

  2007cites this paper
• Analytic Validation of a Quantitative Real-time PCR Assay to Measure CMV Viral Load in Whole Blood

  2007cites this paper
• Simultaneous Quantification of Epstein-Barr Virus, Cytomegalovirus, and Human Herpesvirus 6 DNA in Samples from Transplant Recipients by Multiplex Real-Time PCR Assay (cid:1)

  2007cites this paper
• Analysis of cytomegalovirus (CMV) viremia using the pp65 antigenemia assay, the amplicor CMV test, and a semi‐quantitative polymerase chain reaction test after allogeneic marrow transplantation

  2007cites this paper
• Cytomegalovirus DNA in semen of men seeking fertility evaluation in Abidjan, Côte d’Ivoire

  2007cites this paper
• Comparison of the MagNA pure LC automated system and the RiboPure‐Blood RNA manual method for RNA extraction from multiple myeloma bone marrow samples conserved in an RNA stabilizer

  2007cites this paper
• Prediction of Cytomegalovirus (CMV) Plasma Load from Evaluation of CMV Whole-Blood Load in Samples from Renal Transplant Recipients

  2007influential citation
• Multicentric evaluation of a new commercial cytomegalovirus real-time PCR quantitation assay.

  2007cites this paper
• Quantitative real-time polymerase chain reaction for evaluating DNAemia due to cytomegalovirus, Epstein-Barr virus, and BK virus in solid-organ transplant recipients.

  2007cites this paper
• Quantitative TaqMan assay for the detection and monitoring of cytomegalovirus infection in organ transplant patients.

  2006cites this paper
• Recurrence of symptomatic bacterial vaginosis 12 months after oral metronidazole therapy in HIV-positive and -negative women.

  2006cites this paper
• Development of the loop-mediated isothermal amplification method for rapid detection of cytomegalovirus DNA.

  2006cites this paper
• Development of a quantitative-competitive PCR for quantification of human cytomegalovirus load and comparison with antigenaemia, viraemia and pp67 RNA detection by nucleic acid sequence-based amplification.

  2006cites this paper
• Is human herpesvirus-6 DNA in plasma the right marker for active infection?

  2006cites this paper
• Early detection of plasma cytomegalovirus DNA by real-time PCR after allogeneic hematopoietic stem cell transplantation.

  2006cites this paper
• Real-Time PCR in Clinical Microbiology: Applications for Routine Laboratory Testing

  2006cites this paper
• Reply to Achour et al.

  2006cites this paper
• Polymorphonuclear leukocytes mainly contribute to human herpesvirus-6 load in the peripheral blood of patients.

  2006cites this paper
• Recurrence of Symptomatic Bacterial Vaginosis 12 Months after Oral Metronidazole Therapy in HIV-Positive and-Negative Women

  2006cites this paper
• Whole blood real-time quantitative PCR for cytomegalovirus infection follow-up in transplant recipients.

  2006cites this paper
• Monitoring low cytomegalovirus viremia in transplanted patients by a real‐time PCR on plasma

  2005influential citation
• Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare

  2005cites this paper
• Severe cytomegalovirus ureteritis in a renal allograft recipient with negative CMV monitoring.

  2005cites this paper
• A prospective evaluation of leflunomide therapy for cytomegalovirus disease in renal transplant recipients.

  2005cites this paper
• Use of lyophilized standards for the calibration of a newly developed real time PCR assay for human herpes type six (HHV6) variants A and B.

  2005cites this paper
• Comparison of quantitative PCR and antigenemia in cytomegalovirus infection in renal transplant recipients.

  2005cites this paper
• Clinically‐based determination of safe DNAemia cutoff levels for preemptive therapy or human cytomegalovirus infections in solid organ and hematopoietic stem cell transplant recipients

  2004cites this paper
• Follow-up of 28 HCMV seropositive renal-transplant recipients: comparison of clinical, biological and virological parameters in the groups of treated versus untreated infected patients.

  2004cites this paper
• Quantitative PCR in the diagnosis and monitoring of human cytomegalovirus infection in organ transplant patients

  2004cites this paper
• Use of two real‐time polymerase chain reactions (PCRs) to detect herpes simplex type 1 and 2‐DNA after automated extraction of nucleic acid

  2004cites this paper
• Comparison of Five Methods for Extraction of Legionella pneumophila from Respiratory Specimens

  2004cites this paper