Genetic variation of nucleocapsid genes of porcine epidemic diarrhea virus field strains in China

Porcine epidemic diarrhea virus (PEDV) poses a great threat to the Chinese swine industry. During 2006–2011, 74.0 % (94/127) of samples from 32 swine-raising farms in 15 provinces were positive for PEDV by reverse transcription nested polymerase chain reaction (RT-nested PCR). The sequences of nucleocapsid (N) genes of 32 representative field strains were determined, and the open reading frames (ORFs) of these N genes were 1326 nucleotides long. The N gene sequences were found to be more than 95 % identical to each other. The number of potential phosphorylation sites in the N protein varied from 5 to 12. A phylogenetic tree based on the N genes showed that the 32 Chinese field strains formed three groups.

• Publication year

  2013

• Venue

  Archives of Virology

• Publication date

  2013-02-05

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1007/s00705-013-1608-8PMID 23381394PMCID 7087116
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.

  2011influential reference
• MEGA 5 : Molecular Evolutionary Genetics Analysis using Maximum Likelihood , Evolutionary Distance , and Maximum Parsimony Methods

  2011cited by this paper
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  2010cited by this paper
• Evolution of infectious bronchitis virus in Taiwan: Characterisation of RNA recombination in the nucleocapsid gene

  2010cited by this paper
• Sequence analysis of the partial spike glycoprotein gene of porcine epidemic diarrhea viruses isolated in Korea

  2007cited by this paper
• A new coronavirus-like particle associated with diarrhea in swine

  2005cited by this paper
• Variations in the Nucleocapsid Protein Gene of Infectious Bronchitis Viruses Isolated in Korea

  2005cited by this paper
• Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody

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• Mass Spectroscopic Characterization of the Coronavirus Infectious Bronchitis Virus Nucleoprotein and Elucidation of the Role of Phosphorylation in RNA Binding by Using Surface Plasmon Resonance

  2005cited by this paper
• Induction of anti-viral immune responses by immunization with recombinant-DNA encoded avian coronavirus nucleocapsid protein

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• Detection of porcine epidemic diarrhea virus using polymerase chain reaction and comparison of the nucleocapsid protein genes among strains of the virus.

  1999cited by this paper
• Development of Bi-combined Attenuated Vaccine Against Transmissible Gastroenteritis Virus and Porcine Epidemic Diarrhea Virus

  1999cited by this paper
• Immunohistochemical Detection of Porcine Epidemic Diarrhea Virus Compared to Other Methods

  1998cited by this paper
• Sequence Analysis of the Porcine Epidemic Diarrhea Virus Genome between the Nucleocapsid and Spike Protein Genes Reveals a Polymorphic ORF

  1994cited by this paper
• Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus.

  1993cited by this paper

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  2014cites this paper
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  2014cites this paper
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  2014cites this paper