The Capsid Protein of a Plant Single-stranded RNA Virus Is Modified by O-Linked N-Acetylglucosamine*

Plum pox virus (PPV) is a member of thePotyvirus genus of plant viruses. Labeling with UDP-[3H]galactose and galactosyltransferase indicated that the capsid protein (CP) of PPV is a glycoprotein withN-acetylglucosamine terminal residues. Mass spectrometry analysis of different PPV isolates and mutants revealedO-linked N-acetylglucosamination, a modification barely studied in plant proteins, of serine and/or threonine residues near the amino end of PPV CP. CP of PPV virions is also modified by serine and threonine phosphorylation, as shown by Western blot analysis with anti-phosphoserine and anti-phosphothreonine antibodies. Thus, “yin-yang” glycosylation and phosphorylation may play an important role in the regulation of the different functions in which the potyviral CP is involved.

• Publication year

  2002

• Venue

  Journal of Biological Chemistry

• Publication date

  2002-01-04

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/JBC.M106883200PMID 11606576
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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