3':5'-cyclic AMP: independent induction of amino acid transport by epinephrine in primary cultures of adult rat liver cells.

Liver cells were obtained from adult rats by a collagenase perfusion technique and cultured as monolayers in serum-free media. Epinephrine and isoproterenol both induced large increases in intracellular adenosine 3':5'-monophosphate (cAMP) within 1-2 min whereas epinephrine (but not isoproterenol) induced 2- to 3-fold increases in the rate of alpha-aminoisobutyric acid transport within 2-4 hr after a 1 hr lag. Propranolol abolished the increase in cAMP elicited by epinephrine and isoproterenol, but did not block the induction of alpha-aminoisobutyric acid transport by epinephrine. In contrast, dihydroergotamine abolished and phentolamine diminished the induction of alpha-aminoisobutyric acid transport by epinephrine but did not decrease the stimulation of cAMP levels by epinephrine. Epinephrine dose response curves for cAMP and alpha-aminoisobutyric acid transport were similar. Once exposed to epinephrine, cells became refractory to further stimulation of cAMP levels by epinephrine.

• Publication year

  1977

• Venue

  Proceedings of the National Academy of Sciences of the United States of America

• Publication date

  1977-05-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1073/PNAS.74.1.234PMID 13365PMCID PMC393233
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Studies on the alpha-andrenergic activation of hepatic glucose output. II. Investigation of the roles of adenosine 3':5'-monophosphate and adenosine 3':5'-monophosphate-dependent protein kinase in the actions of phenylephrine in isolated hepatocytes.

  1976influential reference
• In vitro inhibition of cyclic AMP phosphodiesterase by cortisol

  1976cited by this paper
• Reduced sensitivity of the hepatic adenylate cyclase-cyclic AMP system to glucagon during sustained hormonal stimulation.

  1976cited by this paper
• Studies on the role of cyclic guanosine 3':5'-monophosphate and extracellular Ca2+ in the regulation of glycogenolysis in rat liver cells.

  1976cited by this paper
• Ornithine decarboxylase activity and DNA synthesis in Morris hepatomas 5123-C and 7800.

  1976cited by this paper
• Induction of amino acid transport in primary cultures of adult rat liver parenchymal cells by insulin.

  1976cited by this paper
• Stimulation of glycogenolysis and gluconeogenesis by epinephrine independent of its beta-adrenergic function in perfused rat liver.

  1976cited by this paper
• Inductions by hormones added singly, simultaneously or sequentially: what cultured hepatocytes can tell us about metabolic regulation in the whole animal.

  1976cited by this paper
• Studies on the alpha-adrenergic activation of hepatic glucose output. I. Studies on the alpha-adrenergic activation of phosphorylase and gluconeogenesis and inactivation of glycogen synthase in isolated rat liver parenchymal cells.

  1976cited by this paper
• Induction and decay of glucagon-induced amino acid transport in primary cultures of adult rat liver cells: paradoxical effects of cycloheximide and puromycin.

  1976cited by this paper
• Changes in catecholamine- and glucagon-responsive adenylate cyclase activity in preneoplastic rat liver.

  1976cited by this paper
• Hormonal regulation of amino acid transport and gluconeogenesis in primary cultures of adult rat liver parenchymal cells

  1976cited by this paper
• A “permissive” effect of dexamethasone on the glucagon induction of amino acid transport in cultured hepatocytes

  1975cited by this paper
• Glycogenolytic response to glucagon of cultured fetal hepatocytes. Refractoriness following prior exposure to glucagon.

  1975cited by this paper
• Glucose inhibition of epinephrine stimulation of hepatic gluconeogenesis by blockade of the alpha-receptor function.

  1974cited by this paper
• Responses of adipose tissue to sequential lipolytic stimuli.

  1974cited by this paper
• Glucagon and epinephrine activation of adenylate cyclase and glucagon binding in Morris hepatomas.

  1974cited by this paper
• Lack of correlation between catecholamine effects on cyclic adenosine 3':5'-monophosphate and gluconeogenesis in isolated rat liver cells.

  1973cited by this paper
• Biochemical aspects of drug and hormone action on adipose tissue.

  1973cited by this paper
• Hepatic adenyl cyclase: alterations in hormone response during treatment with a chemical carcinogen.

  1972cited by this paper
• Studies on plasma membranes. XIV. Adenyl cyclase in plasma membranes isolated from rat and mouse livers and hepatomas, and its hormone sensitivity.

  1971cited by this paper
• Formation and release of a hormone antagonist by rat adipocytes.

  1971cited by this paper
• Adenyl cyclase activity in Morris hepatomas 7777, 7794A, and 9618A.

  1970cited by this paper
• Glucose Inhibition of Epinephrine Stimulation of Hepatic Gluconeogenesis by Blockade of the a-Receptor Function ( fructose diphosphatase / phosphofructokinase / regulation / glucagon / cyclic purine nucleotides )

  year unknowncited by this paper

• Dual Actions of Apolipoprotein A-I on Glucose-Stimulated Insulin Secretion and Insulin-Independent Peripheral Tissue Glucose Uptake Lead to Increased Heart and Skeletal Muscle Glucose Disposal

  2016cites this paper
• Stable isotope resolved metabolomics of primary human hepatocytes reveals a stressed phenotype

  2012cites this paper
• Amino acid transport in isolated rat hepatocytes

  2005cites this paper
• Effect of theophylline on the transport of tyrosine in cultured B-16 mouse melanoma cells

  2004cites this paper
• Factors Affecting Secretory Protein Production in Primary Cultures of Rat Hepatocytes

  1993cites this paper
• AMINO ACID TRANSPORT IN LIVER

  1992cites this paper
• The isolation and characterization of high-density-lipoprotein subfractions containing apolipoprotein E from human plasma.

  1992cites this paper
• Effect of glucagon and some other alpha and beta adrenergic agonists and antagonists on alanine amino transferase of perfused rat liver

  1991cites this paper
• Epinephrine regulation of amino acid transport in rat hepatocytes isolated during development.

  1990cites this paper
• Effects of norepinephrine on hepatic amino acid metabolism in isolated perfused rat liver.

  1990cites this paper
• Hepatocellular membrane function during chronic burn injury.

  1989cites this paper
• Gluconeogenesis and hypoglycemia.

  1988cites this paper
• Mechanisms of hormonal regulation of hepatic glucose metabolism.

  1987cites this paper
• Gluconeogenesis and its regulation.

  1987cites this paper
• Fatty acid inhibition of hormonal induction of acetyl-coenzyme A carboxylase in hepatocyte monolayers.

  1986cites this paper
• Effectors of amino acid transport processes in animal cell membranes.

  1985cites this paper
• Hormonal effects on L-proline transport in coho salmon (Oncorhynchus kisutch) intestine.

  1985cites this paper
• CHAPTER 7 – Mechanisms Involved in the Actions of Calcium-Dependent Hormones

  1985cites this paper
• Characteristics and hormonal regulation of amino acid transport system A in isolated rat hepatocytes.

  1985cites this paper
• The roles of insulin and glucagon in the regulation of amino acid turnover rate and pool size: in vivo study with [15N]glycine and gas chromatography--mass spectrometry.

  1984cites this paper
• Changes in hormone responsiveness and cyclic AMP metabolism in rat hepatocytes during primary culture and effects of supplementing the medium with insulin and dexamethasone.

  1984cites this paper
• Mechanisms and Possible Biological Role of the Development of Highly Catecholamine-Responsive Adenylate Cyclase Activity in Hepatocytes

  1984cites this paper
• Mechanism of heterologous desensitization of the adenylate cyclase system by glucagon in primary cultures of adult rat hepatocytes.

  1984cites this paper
• Insulin facilitates formation of taurine-conjugated bile acids--analysis using primary cultured hepatocytes.

  1984cites this paper
• Transport of cystine in isolated rat hepatocytes in primary culture.

  1984cites this paper
• [Molecular mechanisms of the interaction of catecholamine with elements of cellular genomes (review)].

  1984cites this paper
• Hormonal regulation of hepatic gluconeogenesis.

  1984cites this paper
• Hormonally produced changes in caeruloplasmin synthesis and secretion in primary cultured rat hepatocytes. Relationship to hepatic copper metabolism.

  1983cites this paper
• Effects of carcinogens on hormonal regulation of gene expression in primary cultures of adult rat hepatocytes.

  1983cites this paper
• Effects of growth factors on hormonal stimulation of amino acid transport in primary cultures of rat hepatocytes.

  1983cites this paper
• Requirement of prolonged presence of a high intracellular level of cyclic AMP for induction of serine dehydratase in primary cultured rat hepatocytes.

  1983cites this paper
• Mechanisms for the emergence of catecholamine-sensitive adenylate cyclase and beta-adrenergic receptors in cultured hepatocytes. Dependence on protein and RNA synthesis and suppression by isoproterenol.

  1983cites this paper
• Differential Regulation of Copper and Zinc Metabolism in Rat Liver Parenchymal Cells in Primary Cultures 1

  1983cites this paper
• The regulation of neutral amino acid transport in mammalian cells.

  1983cites this paper
• Gas-liquid chromatography of free amino acids in the hyaloplasm of rat liver and kidney cells.

  1982cites this paper
• Hormonal Regulation of Copper and Zinc Metabolism in Isolated Rat Liver Parenchymal Cells

  1982cites this paper
• Calcium-Dependent Protein Phosphorylation

  1982cites this paper
• Sodium-gradient-stimulated transport of L-alanine by plasma-membrane vesicles isolated from liver parenchymal cells of fed and starved rats. Crucial role of the adrenal glucocorticoids.

  1982cites this paper
• Activation of aflatoxin B1 by primary cultures of adult rat hepatocytes: effects of hepatocyte density.

  1981cites this paper
• CHAPTER 1 – The Messenger Function of Calcium in Endocrine Systems

  1981cites this paper
• Molecular mechanisms involved in alpha-adrenergic responses.

  1981cites this paper
• Active Ca2+ transport by membrane vesicles from pigeon erythrocytes. Stimulation by amino acids, ATP, GTP, Pi and some other cell constituents.

  1981cites this paper
• Hormonal and metabolic control of hepatic carbohydrate metabolism

  1981cites this paper
• Mechanisms involved in alpha-adrenergic phenomena: role of calcium ions in actions of catecholamines in liver and other tissues.

  1980cites this paper
• Glucocorticoid and catecholamine stimulation of amino acid transport in rat hepatocytes. Synthesis of a high-affinity component.

  1980cites this paper
• The effect of cyclic AMP on glycoprotein secretion in isolated rat hepatocytes.

  1980cites this paper
• Desensitization of primary cultures of adult rat liver parenchymal cells to stimulation of adenosine 3',5'-monophosphate production by glucagon and epinephrine.

  1980cites this paper
• THE SIGNIFICANCE OF DIFFERENCES BETWEEN FRESH CELL SUSPENSIONS AND FRESH OR MAINTAINED MONOLAYERS *

  1980cites this paper
• Independent induction of tyrosine aminotransferase activity by dexamethasone and glucagon in isolated rat liver parenchymal cells in suspension and in monolayer culture in serum-free media.

  1980cites this paper
• Calcium-dependent hormonal regulation of amino acid transport and cyclic AMP accumulation in rat hepatocyte monolayer cultures.

  1980cites this paper
• PROTEIN PHOSPHORYLATION—INVOLVEMENT IN BRAIN FUNCTION*

  1980cites this paper
• The significance of differences between fresh cell suspensions and fresh or maintained monolayers.

  1980cites this paper
• Comparative studies of alanine and alpha-aminoisobutyric acid uptake by freshly isolated rat liver cells.

  1979cites this paper
• Inhibition of lipolysis in hamster epididymal adipocytes by selective alpha-adrenergic agents. Evidence for cyclic AMP-dependent and independent mechanisms.

  1979cites this paper
• Insulin and glucagon stimulation of amino acid transport in isolated rat hepatocytes. Synthesis of a high affinity component of transport.

  1979cites this paper
• Mechanisms involved in alpha-adrenergic effects of catecholamines on liver metabolism.

  1979cites this paper
• Evidence for two alpha-adrenergic binding sites in liver plasma membranes. Studies with [3H]epinephrine and [3H]dihydroergocryptine.

  1979cites this paper
• The regulation of adenosine 3':5'-cyclic monophosphate accumulation in glia by alpha-adrenergic agonists.

  1979cites this paper
• Mechanisms involved in effects of catecholamines on liver carbohydrate metabolism.

  1979cites this paper
• Affinity labelling of alpha-adrenoceptors in intact liver cells by [3H]phenoxybenzamine.

  1979cites this paper
• Electrogenic amino acid transport.

  1979cites this paper
• Adenosine 3',5'-monophosphate-dependent and -independent inhibitory effects of epinephrine on insulin release in rat pancreatic islets.

  1978cites this paper
• The regulation of amino acid transport in animal cells.

  1978cites this paper
• Zinc accumulation and metabolism in primary cultures of adult rat liver cells. Regulation by glucocorticoids.

  1978cites this paper
• Effect of catecholamines on amino acid transport in isolated rat hepatocytes.

  1978cites this paper
• Adrenoceptors.

  1978cites this paper
• Hormonal control of hepatic gluconeogenesis.

  1978cites this paper
• DNA repair in primary cultures of rat hepatocytes.

  1978cites this paper
• Effect of glucocorticoids on amino acid transport in isolated rat hepatocytes.

  1977cites this paper
• Hormonal induction of enzyme functions, cyclic AMP levels and AIB transport in Morris hepatomas and in normal liver systems.

  1977cites this paper