Impact of polysialylated CD56 on natural killer cell cytotoxicity

BackgroundSiglec-7, a sialic acid binding inhibitory receptor expressed by NK cells is masked in vivo by a so far unknown ligand. It shows a strong binding prevalence for α-2,8-linked disialic acids in vitro.ResultsHere we describe the expression of PSA-NCAM (α-2,8-linked polysialic acid modified NCAM) on functional adult peripheral blood natural killer cells and examine its possible role in masking Siglec-7. Unmasking of Siglec-7 using Clostridium perfringens neuraminidase massively reduces NK cell cytotoxicity. By contrast a specific removal of PSA using Endo-NF does not lead to a reduction of NK cell cytotoxicity.ConclusionThe results presented here therefore indicate that PSA-NCAM is not involved in masking Siglec-7.

• Publication year

  2007

• Venue

  BMC Immunology

• Publication date

  2007-08-06

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1186/1471-2172-8-13PMID 17683591PMCID 1976417
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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