SCIENCE@home

Influenza A virus infection increases IgE production and airway responsiveness in aerosolized antigen-exposed mice

Shunsuke Suzuki,Yuzo Suzuki,N. Yamamoto,Y. Matsumoto,A. Shirai,T. Okubo

Published 1998 in Journal of Allergy and Clinical Immunology

ABSTRACT

Background: Respiratory viral infection is known clinically to promote sensitization to antigen inhalation and the development of asthma. Objective: The purpose of this investigation was to determine whether influenza type A virus infection enhances inhalation sensitization and increases airway responsiveness in mice. Methods: Mice were infected by intranasal inoculation with influenza A viruses (strains: H1 N1 and H3 N2 ) or PBS. Animals were exposed to aerosols of ovalbumin on day 3. Two weeks after ovalbumin sensitization, mice were challenged with ovalbumin aerosols; 24 hours later, airway responsiveness (AR) to inhaled methacholine, levels of ovalbumin-specific IgE, and bronchoalveolar lavage fluid (BALF) were examined. Results: Neither influenza A virus (H1 N1 nor H3 N2 ) alone nor ovalbumin sensitization alone caused changes in AR or IgE. However, ovalbumin sensitization after inoculation with either influenza A virus increased AR and levels of ovalbumin-specific IgE. On BALF-cell analysis, ovalbumin sensitization after inoculation with influenza virus A increased the number of lymphocytes but not the number of eosinophils. No difference in AR or IgE levels was observed between the 2 strains of influenza A viruses. Immmunostaining of BALF cells showed an increase in T cells, especially CD8+ cells, with ovalbumin sensitization after inoculation with influenza virus A. Conclusion: Infection by influenza A virus enhances sensitization to inhaled antigens and airway responsiveness in mice by means of mechanisms including CD8+ cells and antigen-specific IgE. (J Allergy Clin Immunol 1998;102:732-40.)

PUBLICATION RECORD

CITATION MAP

EXTRACTION MAP

CLAIMS

  • No claims are published for this paper.

CONCEPTS

  • No concepts are published for this paper.

REFERENCES

Showing 1-43 of 43 references · Page 1 of 1

CITED BY

Showing 1-64 of 64 citing papers · Page 1 of 1