Biosynthesis of vitamin B12 Formation of free 5,6‐dimethylbenzimidazole and α‐ribazole from riboflavin by Propionibacterium freudenreichii

In experiments with radioactive tracers it was shown that the DBI*-moiety of vitamin Blz is formed from riboflavin [l] . Thereby the C-l’ of riboflavin is transformed into C:2 of DBI [2]. These studies were carried out with Propionibacterium shemanii which produces vitamin Blz. In order to isolate the DBI the vitamin Blz was degraded by acid hydrolysis. Until now DBI was never found in free form in vitamin Blz synthesizing bacteria but only as a component of vitamin Blz. In this publication experiments are described demonstrating that cells of P. freudenreichii but not of P. shermanii, grown in the absence of a cobalt salt, transform riboflavin into free DBI, which can be isolated from the incubation medium.

• Publication year

  1977

• Venue

  FEBS Letters

• Publication date

  1977-08-15

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/0014-5793(77)80470-5PMID 891984
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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