Identification of suitable reference genes for gene expression studies by qRT-PCR in the blister beetle Mylabris cichorii

Abstract The blister beetle Mylabris cichorii L. (Coleoptera: Meloidae) is a traditional medicinal insect recorded in the Chinese Pharmacopoeia. It synthesizes cantharidin, which kills cancer cells efficiently. Only males produce large amounts of cantharidin. Reference genes are required as endogenous controls for the analysis of differential gene expression in M. cichorii. Our study chose 10 genes as candidate reference genes. The stability of expression of these genes was analyzed by quantitative PCR and determined with two algorithms, geNorm and Normfinder. We recommend UBE3A and RPL22e as suitable reference genes in females and UBE3A, TAF5, and RPL22e in males.

• Publication year

  2014

• Venue

  Journal of Insect Science

• Publication date

  2014-07-18

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1673/031.014.94PMID 25368050PMCID 4212844
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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