Ia antigenic specificities are oligosaccharide in nature: hapten- inhibition studies

We have previously reported that the Ia specificities, coded for by the I region within the H-2 complex, appear to consist predominantly of carbohydrate. This conclusion was reached by examining low molecular weight Ia-bearing oligosacharides isolated from mouse serum. We now report hapten-inhibition studies which indicate that the binding of both allogeneic and xenogeneic anti-Ia antibodies to the Ia glycoproteins found predominantly on B lymphocytes can be specifically inhibited by certain free sugars. Both inhibition assays revealed that the specificity for the following Ia antigens resides predominantly in the following sugars: (a) Ia.1: N-acetyl-D-mannosamine or related sugars; (b) Ia.3: alpha-D-galactose and related sugars; (c) Ia.7: L- fucose; and (d) Ia.15: N-acetyl-D-glucosamine. It seems likely that these sugars are found at the terminal nonreducing ends of the carbohydrate portion of the Ia-bearing glycoproteins present in the lymphocyte membrane. In contrast, several public and private H-2 antigenic specificities did not appear to be sugar defined. These studies imply that at least some of the Ia genes from both the I-A and I-C subregions of the I region code for glycosyl transferases which modify oligosaccharide structure and impart specificity to the Ia antigens by alteration of their terminal sugar residues.

• Publication year

  1977

• Venue

  Journal of Experimental Medicine

• Publication date

  1977-04-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1084/JEM.145.4.1039PMID 67165PMCID 2180643
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• The relevant Ia determinants likely reside at terminal nonreducing sugar ends on Ia glycoproteins, and some Ia genes from the I-A and I-C subregions likely encode glycosyl transferases that alter those terminal residues.

  Confidence 0.94

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• Ia.1, Ia.3, Ia.7, and Ia.15 are predominantly associated with N-acetyl-D-mannosamine-related sugars, alpha-D-galactose-related sugars, L-fucose, and N-acetyl-D-glucosamine, respectively.

  Confidence 0.97

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• Hapten-inhibition assays show that binding of allogeneic and xenogeneic anti-Ia antibodies to Ia glycoproteins can be specifically blocked by certain free sugars.

  Confidence 0.98

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review

• anti-ia antibodies

  antibody

  Antibodies, from allogeneic or xenogeneic sources, that recognize Ia determinants.

  Aliases: allogeneic anti-Ia antibodies, xenogeneic anti-Ia antibodies

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• glycosyl transferases

  enzyme

  Enzymes that add or alter sugar residues on glycoconjugates.

  Aliases: glycosyltransferases

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• hapten-inhibition studies

  experimental assay

  Binding assays that use free sugars to test whether they block antibody interaction with Ia antigens.

  Aliases: hapten inhibition assays, inhibition assays

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• ia.1

  antigen specificity

  An Ia antigenic specificity examined in the inhibition assays.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• ia.15

  antigen specificity

  An Ia antigenic specificity examined in the inhibition assays.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• ia.3

  antigen specificity

  An Ia antigenic specificity examined in the inhibition assays.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• ia.7

  antigen specificity

  An Ia antigenic specificity examined in the inhibition assays.

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• i-a and i-c subregions

  genetic region

  Mouse H-2 I-region subregions implicated here as sources of Ia genes.

  Aliases: I-A/I-C subregions, I-A subregion and I-C subregion

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• ia glycoproteins

  glycoprotein

  B-lymphocyte membrane glycoproteins that carry Ia determinants.

  Aliases: Ia-bearing glycoproteins

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review
• terminal nonreducing ends

  carbohydrate feature

  The outermost nonreducing sugar termini of an oligosaccharide chain.

  Aliases: terminal sugar residues, nonreducing sugar termini

  박진우 (dztg5apj7m) extraction뀨 (7c402c1b98) reviewB (s683577b42) review

• Structure of the Carbohydrate Units of IgA, Immunoglobulin

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