Characterization of the In Situ Ecophysiology of Novel Phylotypes in Nutrient Removal Activated Sludge Treatment Plants

An in depth understanding of the ecology of activated sludge nutrient removal wastewater treatment systems requires detailed knowledge of the community composition and metabolic activities of individual members. Recent 16S rRNA gene amplicon surveys of activated sludge wastewater treatment plants with nutrient removal indicate the presence of a core set of bacterial genera. These organisms are likely responsible for the bulk of nutrient transformations underpinning the functions of these plants. While the basic activities of some of these genera in situ are known, there is little to no information for the majority. This study applied microautoradiography coupled with fluorescence in situ hybridization (MAR-FISH) for the in situ characterization of selected genus-level-phylotypes for which limited physiological information is available. These included Sulfuritalea and A21b, both within the class Betaproteobacteria, as well as Kaga01, within sub-group 10 of the phylum Acidobacteria. While the Sulfuritalea spp. were observed to be metabolically versatile, the A21b and Kaga01 phylotypes appeared to be highly specialized.

• Publication year

  2015

• Venue

  PLoS ONE

• Publication date

  2015-09-04

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1371/journal.pone.0136424PMID 26340564PMCID 4560404
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Identification of active denitrifiers in full-scale nutrient removal wastewater treatment systems.

  2016cited by this paper
• Studies of the Ecophysiology of Single Cells in Microbial Communities by (Quantitative) Microautoradiography and Fluorescence In Situ Hybridization (MAR-FISH)

  2015cited by this paper
• The activated sludge ecosystem contains a core community of abundant organisms

  2015influential reference
• MiDAS: the field guide to the microbes of activated sludge

  2015influential reference
• Back to Basics – The Influence of DNA Extraction and Primer Choice on Phylogenetic Analysis of Activated Sludge Communities

  2015cited by this paper
• Genome sequences of rare, uncultured bacteria obtained by differential coverage binning of multiple metagenomes

  2013cited by this paper
• High and stable substrate specificities of microorganisms in enhanced biological phosphorus removal plants.

  2013cited by this paper
• ‘Candidatus Competibacter’-lineage genomes retrieved from metagenomes reveal functional metabolic diversity

  2013cited by this paper
• Ribosomal Database Project: data and tools for high throughput rRNA analysis

  2013cited by this paper
• Population dynamics of bacteria involved in enhanced biological phosphorus removal in Danish wastewater treatment plants.

  2013cited by this paper
• The Microbial Database for Danish wastewater treatment plants with nutrient removal (MiDas-DK) - a tool for understanding activated sludge population dynamics and community stability.

  2013cited by this paper
• The SILVA ribosomal RNA gene database project: improved data processing and web-based tools

  2012cited by this paper
• Non-target sites with single nucleotide insertions or deletions are frequently found in 16S rRNA sequences and can lead to false positives in fluorescence in situ hybridization (FISH).

  2011cited by this paper
• 454 Pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants

  2011cited by this paper
• A metagenome of a full-scale microbial community carrying out enhanced biological phosphorus removal

  2011cited by this paper
• mathFISH, a Web Tool That Uses Thermodynamics-Based Mathematical Models for In Silico Evaluation of Oligonucleotide Probes for Fluorescence In Situ Hybridization

  2010cited by this paper
• Microbial Ecology of Activated Sludge

  2010cited by this paper
• A conceptual ecosystem model of microbial communities in enhanced biological phosphorus removal plants.

  2010cited by this paper
• A strictly anaerobic betaproteobacterium Georgfuchsia toluolica gen. nov., sp. nov. degrades aromatic compounds with Fe(III), Mn(IV) or nitrate as an electron acceptor.

  2009cited by this paper
• Double Labeling of Oligonucleotide Probes for Fluorescence In Situ Hybridization (DOPE-FISH) Improves Signal Intensity and Increases rRNA Accessibility

  2009cited by this paper
• Substrate-dependent denitrification of abundant probe-defined denitrifying bacteria in activated sludge.

  2008cited by this paper
• Acidobacteria Phylum Sequences in Uranium-Contaminated Subsurface Sediments Greatly Expand the Known Diversity within the Phylum

  2007cited by this paper
• In situ detection of novel Acidobacteria in microbial mats from a chemolithoautotrophically based cave ecosystem (Lower Kane Cave, WY, USA).

  2007cited by this paper
• Greengenes: Chimera-checked 16S rRNA gene database and workbench compatible in ARB

  2006cited by this paper
• Greengenes, a Chimera-Checked 16S rRNA Gene Database and Workbench Compatible with ARB

  2006cited by this paper
• Isotope Labeling and Microautoradiography of Active Heterotrophic Bacteria on the Basis of Assimilation of 14CO2

  2005cited by this paper
• Molecular microbial ecology

  2005cited by this paper
• Advances in microscopy: microautoradiography of single cells.

  2005cited by this paper
• ARB: a software environment for sequence data.

  2004cited by this paper
• Fluorescence in situ hybridization for the detection of prokaryotes

  2004cited by this paper
• In Situ Characterization ofNitrospira-Like Nitrite-Oxidizing Bacteria Active in Wastewater Treatment Plants

  2001cited by this paper
• Unlabeled Helper Oligonucleotides Increase the In Situ Accessibility to 16S rRNA of Fluorescently Labeled Oligonucleotide Probes

  2000cited by this paper
• Combination of Fluorescent In Situ Hybridization and Microautoradiography—a New Tool for Structure-Function Analyses in Microbial Ecology

  1999cited by this paper
• Detection and in situ identification of representatives of a widely distributed new bacterial phylum.

  1997cited by this paper
• Iron reduction in activated sludge measured with different extraction techniques

  1996cited by this paper
• Optimizing fluorescent in situ hybridization with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms.

  1993cited by this paper
• Phylogenetic Oligodeoxynucleotide Probes for the Major Subclasses of Proteobacteria: Problems and Solutions

  1992cited by this paper

• Coupled N and P cycling as driven by microbial taxa and interactions

  2026cites this paper
• Bacterial community composition and function in different habitats in Antarctic Fildes region revealed by high-throughput sequencing

  2025cites this paper
• Influence of season on the microbial population dynamics of activated sludge.

  2024cites this paper
• Viral community distribution, assembly mechanism, and associated hosts in an industrial park wastewater treatment plant.

  2024cites this paper
• Effect of biofilm thickness on the activity and community composition of phosphorus accumulating bacteria in a moving bed biofilm reactor.

  2023cites this paper
• Exploring the microbial influence on seasonal nitrous oxide concentration in a full-scale wastewater treatment plant using metagenome assembled genomes.

  2022cites this paper
• High Diversity and Functional Potential of Undescribed “Acidobacteriota” in Danish Wastewater Treatment Plants

  2021cites this paper
• Systems Microbiology and Engineering of Aerobic-Anaerobic Ammonium Oxidation

  2020cites this paper
• Microbial Shifts Following Five Years of Cover Cropping and Tillage Practices in Fertile Agroecosystems

  2020cites this paper
• Comparison of shape quantification methods for genomic prediction, and genome-wide association study of sorghum seed morphology

  2019cites this paper
• Antioxidant and Protective Effects of Schinus terebinthifolius Raddi Against Doxorubicin-Induced Toxicity

  2018cites this paper
• Plasmid-normalized quantification of relative mitochondrial DNA copy number

  2018cites this paper
• Hepatitis E virus and related viruses in wild, domestic and zoo animals: A review

  2018cites this paper
• Effect of temperature on microbial diversity and nitrogen removal performance of an anammox reactor treating anaerobically pretreated municipal wastewater.

  2018cites this paper
• Micro-electrolysis/retinervus luffae-based simultaneous autotrophic and heterotrophic denitrification for low C/N wastewater treatment

  2017cites this paper